DOI: 10.1159/000078201. Spatial genome organization during T-cell differentiation

Spatial genome organization during T-cell differentiation

10.1159/000078201

Crossref journal-article

S. Karger AG

Cytogenetic and Genome Research (127)

Abstract

The spatial organization of genomes within the mammalian cell nucleus is non-random. The functional relevance of spatial genome organization might be in influencing gene expression programs as cells undergo changes during development and differentiation. To gain insight into the plasticity of genomes in space and time and to correlate the activity of specific genes with their nuclear position, we systematically analyzed the spatial genome organization in differentiating mouse T-cells. We find significant global reorganization of centromeres, chromosomes and gene loci during the differentiation process. Centromeres were repositioned from a preferentially internal distribution in undifferentiated cells to a preferentially peripheral position in differentiated CD4+ and CD8+ cells. Chromosome 6, containing the differentially expressed T-cell markers CD4 and CD8, underwent differential changes in position depending on whether cells differentiated into CD4+ or CD8+ thymocytes. Similarly, the two marker loci CD4 and CD8 showed distinct behavior in their position relative to the chromosome 6 centromere at various stages of differentiation. Our results demonstrate that significant spatial genome reorganization occurs during differentiation and indicate that the relationship between dynamic genome topology and single gene regulation is highly complex.

Bibliography

Kim, S. H., McQueen, P. G., Lichtman, M. K., Shevach, E. M., Parada, L. A., & Misteli, T. (2004). Spatial genome organization during T-cell differentiation. Cytogenetic and Genome Research, 105(2â4), 292â301. Portico.

Authors 6

S.H. Kim (first)
P.G. McQueen (additional)
M.K. Lichtman (additional)
E.M. Shevach (additional)
L.A. Parada (additional)
T. Misteli (additional)

References 43 Referenced 93

Dates

Type	When
Created	21 years, 1 month ago (July 5, 2004, 11:11 a.m.)
Deposited	4 months, 1 week ago (April 23, 2025, 11:12 p.m.)
Indexed	4 months ago (April 25, 2025, 12:09 a.m.)
Issued	21 years, 7 months ago (Jan. 1, 2004)
Published	21 years, 7 months ago (Jan. 1, 2004)
Published Online	21 years, 1 month ago (July 14, 2004)
Published Print	21 years, 7 months ago (Jan. 1, 2004)

Funders 0

None

BibTeX

@article{Kim_2004, title={Spatial genome organization during T-cell differentiation}, volume={105}, ISSN={1424-859X}, url={http://dx.doi.org/10.1159/000078201}, DOI={10.1159/000078201}, number={2–4}, journal={Cytogenetic and Genome Research}, publisher={S. Karger AG}, author={Kim, S.H. and McQueen, P.G. and Lichtman, M.K. and Shevach, E.M. and Parada, L.A. and Misteli, T.}, year={2004}, pages={292–301} }

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