DOI: 10.1126/science.1078572. Maintenance of Stable Heterochromatin Domains by Dynamic HP1 Binding

Maintenance of Stable Heterochromatin Domains by Dynamic HP1 Binding

10.1126/science.1078572

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and thus is a candidate for establishing and maintaining the transcriptionally repressive heterochromatin structure. Here we demonstrate that maintenance of stable heterochromatin domains in living cells involves the transient binding and dynamic exchange of HP1 from chromatin. HP1 exchange kinetics correlate with the condensation level of chromatin and are dependent on the histone methyltransferase Suv39h. The chromodomain and the chromoshadow domain of HP1 are both required for binding to native chromatin in vivo, but they contribute differentially to binding in euchromatin and heterochromatin. These data argue against HP1 repression of transcription by formation of static, higher order oligomeric networks but support a dynamic competition model, and they demonstrate that heterochromatin is accessible to regulatory factors.

Bibliography

Cheutin, T., McNairn, A. J., Jenuwein, T., Gilbert, D. M., Singh, P. B., & Misteli, T. (2003). Maintenance of Stable Heterochromatin Domains by Dynamic HP1 Binding. Science, 299(5607), 721â725.

Authors 6

Thierry Cheutin (first)
Adrian J. McNairn (additional)
Thomas Jenuwein (additional)
David M. Gilbert (additional)
Prim B. Singh (additional)
Tom Misteli (additional)

References 27 Referenced 525

10.1016/S0959-437X(02)00284-8
10.1126/science.1060118
10.1126/science.1063127
10.1126/science.1074973
10.1016/S0092-8674(01)00542-6
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10.1016/0378-1119(94)90619-X
Materials and methods are available as supporting material on Science Online.
10.1016/S1097-2765(01)00218-0
Kirschmann D. A., et al., Cancer Res. 60, 3359 (2000). / Cancer Res. by Kirschmann D. A. (2000)
T. Cheutin et al. data not shown.
10.1007/s004120050372
10.1016/S0960-9822(01)00390-6
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10.1093/emboj/19.23.6558
We thank T. Karpova for technical support. Imaging was performed at the NCI Fluorescence Imaging Facility. T.M. is a Keith R. Porter Fellow.

Dates

Type	When
Created	22 years, 7 months ago (Jan. 30, 2003, 4:37 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 11:43 p.m.)
Indexed	1 week, 5 days ago (Aug. 21, 2025, 12:55 p.m.)
Issued	22 years, 7 months ago (Jan. 31, 2003)
Published	22 years, 7 months ago (Jan. 31, 2003)
Published Print	22 years, 7 months ago (Jan. 31, 2003)

Funders 0

None

BibTeX

@article{Cheutin_2003, title={Maintenance of Stable Heterochromatin Domains by Dynamic HP1 Binding}, volume={299}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1078572}, DOI={10.1126/science.1078572}, number={5607}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Cheutin, Thierry and McNairn, Adrian J. and Jenuwein, Thomas and Gilbert, David M. and Singh, Prim B. and Misteli, Tom}, year={2003}, month=jan, pages={721–725} }

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