DOI: 10.7554/elife.34410. Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb

Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb

10.7554/elife.34410

Crossref journal-article

eLife Sciences Publications, Ltd

eLife (4374)

Abstract

During development, coordinated cell behaviors orchestrate tissue and organ morphogenesis. Detailed descriptions of cell lineages and behaviors provide a powerful framework to elucidate the mechanisms of morphogenesis. To study the cellular basis of limb development, we imaged transgenic fluorescently-labeled embryos from the crustacean Parhyale hawaiensis with multi-view light-sheet microscopy at high spatiotemporal resolution over several days of embryogenesis. The cell lineage of outgrowing thoracic limbs was reconstructed at single-cell resolution with new software called Massive Multi-view Tracker (MaMuT). In silico clonal analyses suggested that the early limb primordium becomes subdivided into anterior-posterior and dorsal-ventral compartments whose boundaries intersect at the distal tip of the growing limb. Limb-bud formation is associated with spatial modulation of cell proliferation, while limb elongation is also driven by preferential orientation of cell divisions along the proximal-distal growth axis. Cellular reconstructions were predictive of the expression patterns of limb development genes including the BMP morphogen Decapentaplegic.

Bibliography

Wolff, C., Tinevez, J.-Y., Pietzsch, T., Stamataki, E., Harich, B., Guignard, L., Preibisch, S., Shorte, S., Keller, P. J., Tomancak, P., & Pavlopoulos, A. (2018). Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb. ELife, 7. CLOCKSS.

Authors 11

Carsten Wolff (first)
Jean-Yves Tinevez (additional)
Tobias Pietzsch (additional)
Evangelia Stamataki (additional)
Benjamin Harich (additional)
Léo Guignard (additional)
Stephan Preibisch (additional)
Spencer Shorte (additional)
Philipp J Keller (additional)
Pavel Tomancak (additional)
Anastasios Pavlopoulos (additional)

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Dates

Type	When
Created	7 years, 5 months ago (March 29, 2018, noon)
Deposited	1 year, 10 months ago (Oct. 11, 2023, 8:52 p.m.)
Indexed	3 weeks, 3 days ago (Aug. 6, 2025, 8:13 a.m.)
Issued	7 years, 5 months ago (March 29, 2018)
Published	7 years, 5 months ago (March 29, 2018)
Published Online	7 years, 5 months ago (March 29, 2018)

Funders 8

Howard Hughes Medical Institute 10.13039/100000011
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2. HHMI
H2020 Marie Skłodowska-Curie Actions 10.13039/100010665
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1. H2020 Excellent Science - Marie Skłodowska-Curie Actions
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1. FP7-IEF 302235
Max Planck Institute of Molecular Cell Biology and Genetics
European Research Council 10.13039/501100000781
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1. 260746
Einstein Stiftung Berlin 10.13039/501100006188
Region: Europe
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BibTeX

@article{Wolff_2018, title={Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb}, volume={7}, ISSN={2050-084X}, url={http://dx.doi.org/10.7554/elife.34410}, DOI={10.7554/elife.34410}, journal={eLife}, publisher={eLife Sciences Publications, Ltd}, author={Wolff, Carsten and Tinevez, Jean-Yves and Pietzsch, Tobias and Stamataki, Evangelia and Harich, Benjamin and Guignard, Léo and Preibisch, Stephan and Shorte, Spencer and Keller, Philipp J and Tomancak, Pavel and Pavlopoulos, Anastasios}, year={2018}, month=mar }

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  "abstract": "<jats:p>During development, coordinated cell behaviors orchestrate tissue and organ morphogenesis. Detailed descriptions of cell lineages and behaviors provide a powerful framework to elucidate the mechanisms of morphogenesis. To study the cellular basis of limb development, we imaged transgenic fluorescently-labeled embryos from the crustacean Parhyale hawaiensis with multi-view light-sheet microscopy at high spatiotemporal resolution over several days of embryogenesis. The cell lineage of outgrowing thoracic limbs was reconstructed at single-cell resolution with new software called Massive Multi-view Tracker (MaMuT). In silico clonal analyses suggested that the early limb primordium becomes subdivided into anterior-posterior and dorsal-ventral compartments whose boundaries intersect at the distal tip of the growing limb. Limb-bud formation is associated with spatial modulation of cell proliferation, while limb elongation is also driven by preferential orientation of cell divisions along the proximal-distal growth axis. Cellular reconstructions were predictive of the expression patterns of limb development genes including the BMP morphogen Decapentaplegic.</jats:p>",
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