DOI: 10.7554/elife.23006. Using the Volta phase plate with defocus for cryo-EM single particle analysis

Using the Volta phase plate with defocus for cryo-EM single particle analysis

10.7554/elife.23006

Crossref journal-article

eLife Sciences Publications, Ltd

eLife (4374)

Abstract

Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybrid approach simplifies the experiment and increases the data acquisition speed. It also removes the resolution limit inherent to the in-focus method thus allowing 3D reconstructions with resolutions better than 3 Å.

Bibliography

Danev, R., Tegunov, D., & Baumeister, W. (2017). Using the Volta phase plate with defocus for cryo-EM single particle analysis. ELife, 6. CLOCKSS.

Authors 3

Radostin Danev (first)
Dimitry Tegunov (additional)
Wolfgang Baumeister (additional)

References 19 Referenced 118

10.1016/j.str.2012.08.026 / Structure / Movies of ice-embedded particles enhance resolution in electron cryo-microscopy by Campbell (2012)
10.7554/eLife.06380 / eLife / 2.8 å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy by Campbell (2015)
10.1016/j.cell.2015.03.050 / Cell / A primer to single-particle cryo-electron microscopy by Cheng (2015)
10.1093/nar/gkw708 / Nucleic Acids Research / 3.9 å structure of the nucleosome core particle determined by phase-plate cryo-EM by Chua (2016)
10.7554/eLife.13046 / eLife / Cryo-EM single particle analysis with the Volta phase plate by Danev (2016)
10.1073/pnas.1418377111 / PNAS / Volta potential phase plate for in-focus phase contrast transmission electron microscopy by Danev (2014)
10.1016/j.jsb.2015.08.006 / Journal of Structural Biology / Automatic estimation and correction of anisotropic magnification distortion in electron microscopes by Grant (2015)
10.1016/j.jsb.2006.06.006 / Journal of Structural Biology / Bsoft: image processing and molecular modeling for electron microscopy by Heymann (2007)
10.1101/087841 / bioRxiv / Cryo-EM structure of haemoglobin at 3.2 A determined with the volta phase plate by Khoshouei (2016)
10.1038/ncomms10534 / Nature Communications / Volta phase plate cryo-EM of the small protein complex Prx3 by Khoshouei (2016)
10.1101/059717 / bioRxiv / Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2 by Kimanius (2016)
10.1016/j.jsb.2005.07.007 / Journal of Structural Biology / Automated electron microscope tomography using robust prediction of specimen movements by Mastronarde (2005)
10.1016/j.jsb.2016.07.011 / Journal of Structural Biology / Automated tilt series alignment and tomographic reconstruction in IMOD by Mastronarde (2016)
10.1016/j.ultramic.2015.05.017 / Ultramicroscopy / Thon rings from amorphous ice and implications of beam-induced brownian motion in single particle electron cryo-microscopy by McMullan (2015)
10.1016/j.jsb.2015.08.008 / Journal of Structural Biology / CTFFIND4: Fast and accurate defocus estimation from electron micrographs by Rohou (2015)
10.1016/j.jmb.2003.07.013 / Journal of Molecular Biology / Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy by Rosenthal (2003)
10.1016/j.jsb.2012.09.006 / Journal of Structural Biology / RELION: implementation of a Bayesian approach to cryo-EM structure determination by Scheres (2012)
10.7554/eLife.03665 / eLife / Beam-induced motion correction for sub-megadalton cryo-EM particles by Scheres (2014)
10.1101/061960 / bioRxiv / Anisotropic correction of Beam-induced motion for improved Single-particle electron Cryo-microscopy by Zheng (2016)

Dates

Type	When
Created	8 years, 7 months ago (Jan. 21, 2017, 8:01 a.m.)
Deposited	1 year, 10 months ago (Oct. 11, 2023, 8:49 p.m.)
Indexed	4 weeks ago (July 30, 2025, 1:08 p.m.)
Issued	8 years, 7 months ago (Jan. 21, 2017)
Published	8 years, 7 months ago (Jan. 21, 2017)
Published Online	8 years, 7 months ago (Jan. 21, 2017)

Funders 1

Max-Planck-Gesellschaft 10.13039/501100004189
Region: Europe
gov (Research institutes and centers)
Labels4
1. Max Planck Society for the Advancement of Science
2. Max-Planck-Gesellschaft zur Förderung der Wissenschaften
3. Max Planck Society
4. MPG

BibTeX

@article{Danev_2017, title={Using the Volta phase plate with defocus for cryo-EM single particle analysis}, volume={6}, ISSN={2050-084X}, url={http://dx.doi.org/10.7554/elife.23006}, DOI={10.7554/elife.23006}, journal={eLife}, publisher={eLife Sciences Publications, Ltd}, author={Danev, Radostin and Tegunov, Dimitry and Baumeister, Wolfgang}, year={2017}, month=jan }

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