DOI: 10.7554/elife.10180. Structure and conformational states of the bovine mitochondrial ATP synthase by cryo-EM

Structure and conformational states of the bovine mitochondrial ATP synthase by cryo-EM

10.7554/elife.10180

Crossref journal-article

eLife Sciences Publications, Ltd

eLife (4374)

Abstract

Adenosine triphosphate (ATP), the chemical energy currency of biology, is synthesized in eukaryotic cells primarily by the mitochondrial ATP synthase. ATP synthases operate by a rotary catalytic mechanism where proton translocation through the membrane-inserted FO region is coupled to ATP synthesis in the catalytic F1 region via rotation of a central rotor subcomplex. We report here single particle electron cryomicroscopy (cryo-EM) analysis of the bovine mitochondrial ATP synthase. Combining cryo-EM data with bioinformatic analysis allowed us to determine the fold of the a subunit, suggesting a proton translocation path through the FO region that involves both the a and b subunits. 3D classification of images revealed seven distinct states of the enzyme that show different modes of bending and twisting in the intact ATP synthase. Rotational fluctuations of the c8-ring within the FO region support a Brownian ratchet mechanism for proton-translocation-driven rotation in ATP synthases.

Bibliography

Zhou, A., Rohou, A., Schep, D. G., Bason, J. V., Montgomery, M. G., Walker, J. E., Grigorieff, N., & Rubinstein, J. L. (2015). Structure and conformational states of the bovine mitochondrial ATP synthase by cryo-EM. ELife, 4. CLOCKSS.

Authors 8

Anna Zhou (first)
Alexis Rohou (additional)
Daniel G Schep (additional)
John V Bason (additional)
Martin G Montgomery (additional)
John E Walker (additional)
Nikolaus Grigorieff (additional)
John L Rubinstein (additional)

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Dates

Type	When
Created	9 years, 10 months ago (Oct. 6, 2015, 7:43 a.m.)
Deposited	1 year, 10 months ago (Oct. 11, 2023, 8:53 p.m.)
Indexed	20 hours, 44 minutes ago (Aug. 20, 2025, 8:30 a.m.)
Issued	9 years, 10 months ago (Oct. 6, 2015)
Published	9 years, 10 months ago (Oct. 6, 2015)
Published Online	9 years, 10 months ago (Oct. 6, 2015)

Funders 6

Canadian Institutes of Health Research 10.13039/501100000024
Region: Americas
gov (National government)
Labels6
1. Instituts de Recherche en Santé du Canada
2. Canadian Institutes of Health Research (CIHR)
3. CIHR_IRSC
4. Canadian Institutes of Health Research | Ottawa ON
5. CIHR
6. IRSC
Awards1
1. MOP 81294
Medical Research Council 10.13039/501100000265
Region: Europe
gov (National government)
Labels3
1. Medical Research Council (United Kingdom)
2. UK Medical Research Council
3. MRC
Awards1
1. U105663150
Howard Hughes Medical Institute 10.13039/100000011
Region: Americas
pri (Research institutes and centers)
Labels2
1. Howard Hughes Medical Institute Inc
2. HHMI
Canada Research Chairs (Chaires de recherche du Canada)
Canadian Institutes of Health Research 10.13039/501100000024
Region: Americas
gov (National government)
Labels6
1. Instituts de Recherche en Santé du Canada
2. Canadian Institutes of Health Research (CIHR)
3. CIHR_IRSC
4. Canadian Institutes of Health Research | Ottawa ON
5. CIHR
6. IRSC
Awards1
1. postgraduate scholarship
Natural Sciences and Engineering Research Council of Canada 10.13039/501100000038
Region: Americas
gov (National government)
Labels3
1. Conseil de Recherches en Sciences Naturelles et en Génie du Canada
2. NSERC
3. CRSNG
Awards1
1. postgraduate scholarship

BibTeX

@article{Zhou_2015, title={Structure and conformational states of the bovine mitochondrial ATP synthase by cryo-EM}, volume={4}, ISSN={2050-084X}, url={http://dx.doi.org/10.7554/elife.10180}, DOI={10.7554/elife.10180}, journal={eLife}, publisher={eLife Sciences Publications, Ltd}, author={Zhou, Anna and Rohou, Alexis and Schep, Daniel G and Bason, John V and Montgomery, Martin G and Walker, John E and Grigorieff, Nikolaus and Rubinstein, John L}, year={2015}, month=oct }

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