DOI: 10.7554/elife.07380. Structure of a type IV pilus machinery in the open and closed state

Structure of a type IV pilus machinery in the open and closed state

10.7554/elife.07380

Crossref journal-article

eLife Sciences Publications, Ltd

eLife (4374)

Abstract

Proteins of the secretin family form large macromolecular complexes, which assemble in the outer membrane of Gram-negative bacteria. Secretins are major components of type II and III secretion systems and are linked to extrusion of type IV pili (T4P) and to DNA uptake. By electron cryo-tomography of whole Thermus thermophilus cells, we determined the in situ structure of a T4P molecular machine in the open and the closed state. Comparison reveals a major conformational change whereby the N-terminal domains of the central secretin PilQ shift by ∼30 Å, and two periplasmic gates open to make way for pilus extrusion. Furthermore, we determine the structure of the assembled pilus.

Bibliography

Gold, V. A., Salzer, R., Averhoff, B., & KÃ¼hlbrandt, W. (2015). Structure of a type IV pilus machinery in the open and closed state. ELife, 4. CLOCKSS.

Authors 4

Vicki AM Gold (first)
Ralf Salzer (additional)
Beate Averhoff (additional)
Werner Kühlbrandt (additional)

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Dates

Type	When
Created	10 years, 3 months ago (May 21, 2015, 7:33 a.m.)
Deposited	1 year, 10 months ago (Oct. 11, 2023, 8:49 p.m.)
Indexed	3 weeks, 5 days ago (July 30, 2025, 1:07 p.m.)
Issued	10 years, 3 months ago (May 21, 2015)
Published	10 years, 3 months ago (May 21, 2015)
Published Online	10 years, 3 months ago (May 21, 2015)

Funders 2

Max-Planck-Gesellschaft (Max Planck Society) 10.13039/501100004189 Max-Planck-Gesellschaft
Region: Europe
gov (Research institutes and centers)
Labels4
1. Max Planck Society for the Advancement of Science
2. Max-Planck-Gesellschaft zur Förderung der Wissenschaften
3. Max Planck Society
4. MPG
Deutsche Forschungsgemeinschaft (DFG) 10.13039/501100001659 Deutsche Forschungsgemeinschaft
Region: Europe
gov (National government)
Labels3
1. German Research Association
2. German Research Foundation
3. DFG
Awards1
1. AV 9/6-1

BibTeX

@article{Gold_2015, title={Structure of a type IV pilus machinery in the open and closed state}, volume={4}, ISSN={2050-084X}, url={http://dx.doi.org/10.7554/elife.07380}, DOI={10.7554/elife.07380}, journal={eLife}, publisher={eLife Sciences Publications, Ltd}, author={Gold, Vicki AM and Salzer, Ralf and Averhoff, Beate and Kühlbrandt, Werner}, year={2015}, month=may }

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