DOI: 10.7554/elife.06380. 2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy

2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy

10.7554/elife.06380

Crossref journal-article

eLife Sciences Publications, Ltd

eLife (4374)

Abstract

Recent developments in detector hardware and image-processing software have revolutionized single particle cryo-electron microscopy (cryoEM) and led to a wave of near-atomic resolution (typically ∼3.3 Å) reconstructions. Reaching resolutions higher than 3 Å is a prerequisite for structure-based drug design and for cryoEM to become widely interesting to pharmaceutical industries. We report here the structure of the 700 kDa Thermoplasma acidophilum 20S proteasome (T20S), determined at 2.8 Å resolution by single-particle cryoEM. The quality of the reconstruction enables identifying the rotameric conformation adopted by some amino-acid side chains (rotamers) and resolving ordered water molecules, in agreement with the expectations for crystal structures at similar resolutions. The results described in this manuscript demonstrate that single particle cryoEM is capable of competing with X-ray crystallography for determination of protein structures of suitable quality for rational drug design.

Bibliography

Campbell, M. G., Veesler, D., Cheng, A., Potter, C. S., & Carragher, B. (2015). 2.8 Ã resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy. ELife, 4. CLOCKSS.

Authors 5

Melody G Campbell (first)
David Veesler (additional)
Anchi Cheng (additional)
Clinton S Potter (additional)
Bridget Carragher (additional)

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Dates

Type	When
Created	10 years, 5 months ago (March 11, 2015, 8:33 a.m.)
Deposited	1 year, 10 months ago (Oct. 11, 2023, 8:47 p.m.)
Indexed	3 weeks, 4 days ago (July 30, 2025, 1:07 p.m.)
Issued	10 years, 5 months ago (March 11, 2015)
Published	10 years, 5 months ago (March 11, 2015)
Published Online	10 years, 5 months ago (March 11, 2015)

Funders 4

National Institute of General Medical Sciences (NIGMS) 10.13039/100000057 National Institute of General Medical Sciences
Region: Americas
gov (National government)
Labels2
1. U.S. National Institute of General Medical Sciences
2. NIGMS
Awards1
1. GM103310
American Heart Association (AHA) 10.13039/100000968 American Heart Association
Region: Americas
gov (Associations and societies (private and public))
Labels5
1. American Heart Association, Inc.
2. AMERICAN HEART ASSOC.
3. American Heart Assn
4. Association for the Prevention and Relief of Heart Disease
5. AHA
Awards1
1. 14PRE18870036
European Commission Directorate-General for Research and Innovation 10.13039/100004431 Directorate-General for Research and Innovation
Region: Europe
pri (International organizations)
Labels1
1. EC DG Research
Awards1
1. Marie Curie IOF fellowship (273427)
National Institutes of Health (NIH) 10.13039/100000002 National Institutes of Health
Region: Americas
gov (National government)
Labels3
1. Institutos Nacionales de la Salud
2. US National Institutes of Health
3. NIH
Awards1
1. GM103310

BibTeX

@article{Campbell_2015, title={2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy}, volume={4}, ISSN={2050-084X}, url={http://dx.doi.org/10.7554/elife.06380}, DOI={10.7554/elife.06380}, journal={eLife}, publisher={eLife Sciences Publications, Ltd}, author={Campbell, Melody G and Veesler, David and Cheng, Anchi and Potter, Clinton S and Carragher, Bridget}, year={2015}, month=mar }

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