DOI: 10.7554/elife.06197. Protein aggregates are associated with replicative aging without compromising protein quality control

Protein aggregates are associated with replicative aging without compromising protein quality control

10.7554/elife.06197

Crossref journal-article

eLife Sciences Publications, Ltd

eLife (4374)

Abstract

Differentiation of cellular lineages is facilitated by asymmetric segregation of fate determinants between dividing cells. In budding yeast, various aging factors segregate to the aging (mother)-lineage, with poorly understood consequences. In this study, we show that yeast mother cells form a protein aggregate during early replicative aging that is maintained as a single, asymmetrically inherited deposit over the remaining lifespan. Surprisingly, deposit formation was not associated with stress or general decline in proteostasis. Rather, the deposit-containing cells displayed enhanced degradation of cytosolic proteasome substrates and unimpaired clearance of stress-induced protein aggregates. Deposit formation was dependent on Hsp42, which collected non-random client proteins of the Hsp104/Hsp70-refolding machinery, including the prion Sup35. Importantly, loss of Hsp42 resulted in symmetric inheritance of its constituents and prolonged the lifespan of the mother cell. Together, these data suggest that protein aggregation is an early aging-associated differentiation event in yeast, having a two-faceted role in organismal fitness.

Bibliography

Saarikangas, J., & Barral, Y. (2015). Protein aggregates are associated with replicative aging without compromising protein quality control. ELife, 4. CLOCKSS.

Authors 2

Juha Saarikangas (first)
Yves Barral (additional)

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Dates

Type	When
Created	9 years, 9 months ago (Nov. 6, 2015, 6:58 a.m.)
Deposited	1 year, 10 months ago (Oct. 11, 2023, 8:47 p.m.)
Indexed	1 month ago (July 30, 2025, 1:07 p.m.)
Issued	9 years, 9 months ago (Nov. 6, 2015)
Published	9 years, 9 months ago (Nov. 6, 2015)
Published Online	9 years, 9 months ago (Nov. 6, 2015)

Funders 4

FEBS 10.13039/100012623 Federation of European Biochemical Societies
Region: Europe
pri (Associations and societies (private and public))
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Suomen Kulttuurirahasto 10.13039/501100003125
Region: Europe
pri (Trusts, charities, foundations (both public and private))
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1. Finnish Cultural Foundation
2. SKR
Awards1
1. Post Doc Pool
European Research Council (ERC) 10.13039/501100000781 European Research Council
Region: Europe
gov (National government)
Labels1
1. ERC
Awards1
1. BarrAge 250278
Eidgenössische Technische Hochschule Zürich 10.13039/501100003006
Region: Europe
pri (Universities (academic only))
Labels8
1. ETH Zurich
2. ETH Zürich
3. Federal Institute of Technology Zurich
4. ETH Zürich (Eidgenössische Technische Hochschule Zürich)
5. Eidgenössische Technische Hochschule Zürich (Switzerland)
6. Eidgenössische Technische Hochschule Zürich (ETH)
7. ethzurich
8. ETH

BibTeX

@article{Saarikangas_2015, title={Protein aggregates are associated with replicative aging without compromising protein quality control}, volume={4}, ISSN={2050-084X}, url={http://dx.doi.org/10.7554/elife.06197}, DOI={10.7554/elife.06197}, journal={eLife}, publisher={eLife Sciences Publications, Ltd}, author={Saarikangas, Juha and Barral, Yves}, year={2015}, month=nov }

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