Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography
10.7554/elife.04889
Crossref journal-article
eLife Sciences Publications, Ltd
eLife (4374)
Abstract

Chloroplast function is orchestrated by the organelle's intricate architecture. By combining cryo-focused ion beam milling of vitreous Chlamydomonas cells with cryo-electron tomography, we acquired three-dimensional structures of the chloroplast in its native state within the cell. Chloroplast envelope inner membrane invaginations were frequently found in close association with thylakoid tips, and the tips of multiple thylakoid stacks converged at dynamic sites on the chloroplast envelope, implicating lipid transport in thylakoid biogenesis. Subtomogram averaging and nearest neighbor analysis revealed that RuBisCO complexes were hexagonally packed within the pyrenoid, with ∼15 nm between their centers. Thylakoid stacks and the pyrenoid were connected by cylindrical pyrenoid tubules, physically bridging the sites of light-dependent photosynthesis and light-independent carbon fixation. Multiple parallel minitubules were bundled within each pyrenoid tubule, possibly serving as conduits for the targeted one-dimensional diffusion of small molecules such as ATP and sugars between the chloroplast stroma and the pyrenoid matrix.

Bibliography

Engel, B. D., Schaffer, M., Kuhn Cuellar, L., Villa, E., Plitzko, J. M., & Baumeister, W. (2015). Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography. ELife, 4. CLOCKSS.

Authors 6
  1. Benjamin D Engel (first)
  2. Miroslava Schaffer (additional)
  3. Luis Kuhn Cuellar (additional)
  4. Elizabeth Villa (additional)
  5. Jürgen M Plitzko (additional)
  6. Wolfgang Baumeister (additional)
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Dates
Type When
Created 10 years, 7 months ago (Jan. 13, 2015, 8:24 a.m.)
Deposited 1 year, 10 months ago (Oct. 11, 2023, 8:45 p.m.)
Indexed 3 weeks ago (July 30, 2025, 1:08 p.m.)
Issued 10 years, 7 months ago (Jan. 13, 2015)
Published 10 years, 7 months ago (Jan. 13, 2015)
Published Online 10 years, 7 months ago (Jan. 13, 2015)
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@article{Engel_2015, title={Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography}, volume={4}, ISSN={2050-084X}, url={http://dx.doi.org/10.7554/elife.04889}, DOI={10.7554/elife.04889}, journal={eLife}, publisher={eLife Sciences Publications, Ltd}, author={Engel, Benjamin D and Schaffer, Miroslava and Kuhn Cuellar, Luis and Villa, Elizabeth and Plitzko, Jürgen M and Baumeister, Wolfgang}, year={2015}, month=jan }