DOI: 10.7554/elife.01299. A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5 Å resolution

A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5 Å resolution

10.7554/elife.01299

Crossref journal-article

eLife Sciences Publications, Ltd

eLife (4374)

Abstract

Bacteriophage BPP-1 infects and kills Bordetella species that cause whooping cough. Its diversity-generating retroelement (DGR) provides a naturally occurring phage-display system, but engineering efforts are hampered without atomic structures. Here, we report a cryo electron microscopy structure of the BPP-1 head at 3.5 Å resolution. Our atomic model shows two of the three protein folds representing major viral lineages: jellyroll for its cement protein (CP) and HK97-like (‘Johnson’) for its major capsid protein (MCP). Strikingly, the fold topology of MCP is permuted non-circularly from the Johnson fold topology previously seen in viral and cellular proteins. We illustrate that the new topology is likely the only feasible alternative of the old topology. β-sheet augmentation and electrostatic interactions contribute to the formation of non-covalent chainmail in BPP-1, unlike covalent inter-protein linkages of the HK97 chainmail. Despite these complex interactions, the termini of both CP and MCP are ideally positioned for DGR-based phage-display engineering.

Bibliography

Zhang, X., Guo, H., Jin, L., Czornyj, E., Hodes, A., Hui, W. H., Nieh, A. W., Miller, J. F., & Zhou, Z. H. (2013). A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5 Ã resolution. ELife, 2. CLOCKSS.

Authors 9

Xing Zhang (first)
Huatao Guo (additional)
Lei Jin (additional)
Elizabeth Czornyj (additional)
Asher Hodes (additional)
Wong H Hui (additional)
Angela W Nieh (additional)
Jeff F Miller (additional)
Z Hong Zhou (additional)

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Dates

Type	When
Created	11 years, 8 months ago (Dec. 17, 2013, 12:45 p.m.)
Deposited	1 year, 10 months ago (Oct. 11, 2023, 8:41 p.m.)
Indexed	4 weeks, 2 days ago (July 30, 2025, 1:07 p.m.)
Issued	11 years, 8 months ago (Dec. 17, 2013)
Published	11 years, 8 months ago (Dec. 17, 2013)
Published Online	11 years, 8 months ago (Dec. 17, 2013)

Funders 4

National Institutes of Health 10.13039/100000002
Region: Americas
gov (National government)
Labels3
1. Institutos Nacionales de la Salud
2. US National Institutes of Health
3. NIH
Awards1
1. AI046420, GM071940
National Institutes of Health 10.13039/100000002
Region: Americas
gov (National government)
Labels3
1. Institutos Nacionales de la Salud
2. US National Institutes of Health
3. NIH
Awards1
1. AI069838
National Institutes of Health 10.13039/100000002
Region: Americas
gov (National government)
Labels3
1. Institutos Nacionales de la Salud
2. US National Institutes of Health
3. NIH
Awards1
1. AI046420, GM071940
National Institutes of Health 10.13039/100000002
Region: Americas
gov (National government)
Labels3
1. Institutos Nacionales de la Salud
2. US National Institutes of Health
3. NIH
Awards1
1. AI069838

BibTeX

@article{Zhang_2013, title={A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5 Å resolution}, volume={2}, ISSN={2050-084X}, url={http://dx.doi.org/10.7554/elife.01299}, DOI={10.7554/elife.01299}, journal={eLife}, publisher={eLife Sciences Publications, Ltd}, author={Zhang, Xing and Guo, Huatao and Jin, Lei and Czornyj, Elizabeth and Hodes, Asher and Hui, Wong H and Nieh, Angela W and Miller, Jeff F and Zhou, Z Hong}, year={2013}, month=dec }

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