DOI: 10.7554/elife.00951. The structure of the COPII transport-vesicle coat assembled on membranes

The structure of the COPII transport-vesicle coat assembled on membranes

10.7554/elife.00951

Crossref journal-article

eLife Sciences Publications, Ltd

eLife (4374)

Abstract

Coat protein complex II (COPII) mediates formation of the membrane vesicles that export newly synthesised proteins from the endoplasmic reticulum. The inner COPII proteins bind to cargo and membrane, linking them to the outer COPII components that form a cage around the vesicle. Regulated flexibility in coat architecture is essential for transport of a variety of differently sized cargoes, but structural data on the assembled coat has not been available. We have used cryo-electron tomography and subtomogram averaging to determine the structure of the complete, membrane-assembled COPII coat. We describe a novel arrangement of the outer coat and find that the inner coat can assemble into regular lattices. The data reveal how coat subunits interact with one another and with the membrane, suggesting how coordinated assembly of inner and outer coats can mediate and regulate packaging of vesicles ranging from small spheres to large tubular carriers.

Bibliography

Zanetti, G., Prinz, S., Daum, S., Meister, A., Schekman, R., Bacia, K., & Briggs, J. A. (2013). The structure of the COPII transport-vesicle coat assembled on membranes. ELife, 2. CLOCKSS.

Authors 7

Giulia Zanetti (first)
Simone Prinz (additional)
Sebastian Daum (additional)
Annette Meister (additional)
Randy Schekman (additional)
Kirsten Bacia (additional)
John AG Briggs (additional)

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Dates

Type	When
Created	11 years, 11 months ago (Sept. 17, 2013, 11:40 a.m.)
Deposited	1 year, 10 months ago (Oct. 11, 2023, 8:40 p.m.)
Indexed	3 weeks, 4 days ago (Aug. 2, 2025, 1:07 a.m.)
Issued	11 years, 11 months ago (Sept. 17, 2013)
Published	11 years, 11 months ago (Sept. 17, 2013)
Published Online	11 years, 11 months ago (Sept. 17, 2013)

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BibTeX

@article{Zanetti_2013, title={The structure of the COPII transport-vesicle coat assembled on membranes}, volume={2}, ISSN={2050-084X}, url={http://dx.doi.org/10.7554/elife.00951}, DOI={10.7554/elife.00951}, journal={eLife}, publisher={eLife Sciences Publications, Ltd}, author={Zanetti, Giulia and Prinz, Simone and Daum, Sebastian and Meister, Annette and Schekman, Randy and Bacia, Kirsten and Briggs, John AG}, year={2013}, month=sep }

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