DOI: 10.7554/elife.00005. Molecular architecture of human polycomb repressive complex 2

Molecular architecture of human polycomb repressive complex 2

10.7554/elife.00005

Crossref journal-article

eLife Sciences Publications, Ltd

eLife (4374)

Abstract

Polycomb Repressive Complex 2 (PRC2) is essential for gene silencing, establishing transcriptional repression of specific genes by tri-methylating Lysine 27 of histone H3, a process mediated by cofactors such as AEBP2. In spite of its biological importance, little is known about PRC2 architecture and subunit organization. Here, we present the first three-dimensional electron microscopy structure of the human PRC2 complex bound to its cofactor AEBP2. Using a novel internal protein tagging-method, in combination with isotopic chemical cross-linking and mass spectrometry, we have localized all the PRC2 subunits and their functional domains and generated a detailed map of interactions. The position and stabilization effect of AEBP2 suggests an allosteric role of this cofactor in regulating gene silencing. Regions in PRC2 that interact with modified histone tails are localized near the methyltransferase site, suggesting a molecular mechanism for the chromatin-based regulation of PRC2 activity.

Bibliography

Ciferri, C., Lander, G. C., Maiolica, A., Herzog, F., Aebersold, R., & Nogales, E. (2012). Molecular architecture of human polycomb repressive complex 2. ELife, 1. CLOCKSS.

Authors 6

Claudio Ciferri (first)
Gabriel C Lander (additional)
Alessio Maiolica (additional)
Franz Herzog (additional)
Ruedi Aebersold (additional)
Eva Nogales (additional)

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Dates

Type	When
Created	12 years, 9 months ago (Oct. 30, 2012, 12:41 a.m.)
Deposited	1 year, 10 months ago (Oct. 11, 2023, 8:37 p.m.)
Indexed	3 weeks ago (July 30, 2025, 1:07 p.m.)
Issued	12 years, 9 months ago (Oct. 30, 2012)
Published	12 years, 9 months ago (Oct. 30, 2012)
Published Online	12 years, 9 months ago (Oct. 30, 2012)

Funders 14

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ERC advanced grant “Proteomics v3.0”
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National Institute of General Medical Sciences 10.13039/100000057
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12. Commissione europea
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BibTeX

@article{Ciferri_2012, title={Molecular architecture of human polycomb repressive complex 2}, volume={1}, ISSN={2050-084X}, url={http://dx.doi.org/10.7554/elife.00005}, DOI={10.7554/elife.00005}, journal={eLife}, publisher={eLife Sciences Publications, Ltd}, author={Ciferri, Claudio and Lander, Gabriel C and Maiolica, Alessio and Herzog, Franz and Aebersold, Ruedi and Nogales, Eva}, year={2012}, month=oct }

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