DOI: 10.4049/jimmunol.62.4.437. Use of Ultraviolet Absorption Spectroscopy in the Quantitative Precipitin Reaction,

Use of Ultraviolet Absorption Spectroscopy in the Quantitative Precipitin Reaction,

10.4049/jimmunol.62.4.437

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Summary The effects of acid and alkali on several purified plasma proteins employed as antigens, and on their homologous specific precipitates, have been reported. The ultraviolet absorption properties of these antigens and their homologous rabbit antibodies have been shown to be largely dependent upon their tyrosine and tryptophane content. The ultraviolet absorption properties of the rabbit antibodies studied are very similar to those of human serum γ-globulin, and it is suggested that the tyrosine and tryptophane content of the rabbit antibodies is very similar to that of human serum γ-globulin. E 1% 1cm has been calculated for several rabbit antibodies. A method is described for using the spectrophotometer for studies of the quantitative precipitin reaction. The spectrophotometric method of determining total and antibody protein in specific precipitates has been applied to the study of the precipitation of several human and bovine plasma proteins by their homologous rabbit antisera.

Bibliography

Gitlin, D. (1949). Use of Ultraviolet Absorption Spectroscopy in the Quantitative Precipitin Reaction,. The Journal of Immunology, 62(4), 437â451.

Authors 1

David Gitlin (first)

References 0 Referenced 57

None

Dates

Type	When
Created	2 years, 7 months ago (Jan. 1, 2023, 10:30 a.m.)
Deposited	4 months, 2 weeks ago (April 9, 2025, 11:19 p.m.)
Indexed	4 months, 2 weeks ago (April 11, 2025, 7:29 a.m.)
Issued	76 years ago (Aug. 1, 1949)
Published	76 years ago (Aug. 1, 1949)
Published Online	76 years ago (Aug. 1, 1949)
Published Print	76 years ago (Aug. 1, 1949)

Funders 0

None

BibTeX

@article{Gitlin_1949, title={Use of Ultraviolet Absorption Spectroscopy in the Quantitative Precipitin Reaction,}, volume={62}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.62.4.437}, DOI={10.4049/jimmunol.62.4.437}, number={4}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Gitlin, David}, year={1949}, month=aug, pages={437–451} }

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