DOI: 10.4049/jimmunol.178.12.8046. Human Neutrophil Defensins Increase Neutrophil Uptake of Influenza A Virus and Bacteria and Modify Virus-Induced Respiratory Burst Responses

Human Neutrophil Defensins Increase Neutrophil Uptake of Influenza A Virus and Bacteria and Modify Virus-Induced Respiratory Burst Responses

10.4049/jimmunol.178.12.8046

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Human neutrophil peptides (HNPs) are released from granules of neutrophils in response to various activating stimuli and they participate in the killing of bacteria and the stimulation of various inflammatory responses. HNPs also inhibit infectivity of enveloped viruses, including influenza A virus (IAV). In this study, we demonstrate that HNPs increase the uptake of IAV and bacteria by neutrophils. The dimeric HNPs also induced aggregation of IAV and bacterial particles, which may, in part, explain their ability to increase uptake. HNPs did not increase neutrophil respiratory burst responses to IAV. We have recently demonstrated direct interactions of HNPs with surfactant protein D (SP-D), another important effector of innate immunity and antimicrobial host defense. Although HNPs did not alter SP-D-dependent uptake of IAV, they counteracted the ability of SP-D to increase IAV-induced neutrophil H2O2 generation. Our studies reveal previously unappreciated functional effects of HNPs, expand our understanding of the antiviral properties of HNPs, and suggest important interactions between collectins and HNPs in the host response to viruses and bacteria.

Bibliography

Tecle, T., White, M. R., Gantz, D., Crouch, E. C., & Hartshorn, K. L. (2007). Human Neutrophil Defensins Increase Neutrophil Uptake of Influenza A Virus and Bacteria and Modify Virus-Induced Respiratory Burst Responses. The Journal of Immunology, 178(12), 8046â8052.

Authors 5

Tesfaldet Tecle (first)
Mitchell R White (additional)
Don Gantz (additional)
Erika C Crouch (additional)
Kevan L Hartshorn (additional)

References 46 Referenced 87

{'first-page': '227', 'volume-title': 'J. Am. Med. Assoc.', 'year': '2003', 'key': '2025032803201713400_R1'} / J. Am. Med. Assoc. (2003)
{'first-page': '303', 'volume-title': 'Microbiol. Rev.', 'year': '1980', 'key': '2025032803201713400_R2'} / Microbiol. Rev. (1980)
{'first-page': '5868', 'volume-title': 'J. Immunol.', 'year': '2001', 'key': '2025032803201713400_R3'} / J. Immunol. (2001)
{'first-page': '2472', 'volume-title': 'J. Virol.', 'year': '2000', 'key': '2025032803201713400_R4'} / J. Virol. (2000)
{'first-page': '14933', 'volume-title': 'J. Virol.', 'year': '2005', 'key': '2025032803201713400_R5'} / J. Virol. (2005)
{'first-page': '425', 'volume-title': 'J. Gen. Virol.', 'year': '1987', 'key': '2025032803201713400_R6'} / J. Gen. Virol. (1987)
{'first-page': '499', 'volume-title': 'Options for the Control of Influenza', 'year': '1996', 'key': '2025032803201713400_R7'} / Options for the Control of Influenza (1996)
{'first-page': '2040', 'volume-title': 'Clin. Infect. Dis.', 'year': '2001', 'key': '2025032803201713400_R8'} / Clin. Infect. Dis. (2001)
{'first-page': '765', 'volume-title': 'J. Clin. Invest.', 'year': '2005', 'key': '2025032803201713400_R9'} / J. Clin. Invest. (2005)
{'first-page': '1068', 'volume-title': 'J. Virol.', 'year': '1986', 'key': '2025032803201713400_R10'} / J. Virol. (1986)
{'first-page': '519', 'volume-title': 'Chest', 'year': '2002', 'key': '2025032803201713400_R11'} / Chest (2002)
{'first-page': 'L514', 'volume-title': 'Am. J. Physiol.', 'year': '2004', 'key': '2025032803201713400_R12'} / Am. J. Physiol. (2004)
{'first-page': '2686', 'volume-title': 'J. Immunol.', 'year': '1988', 'key': '2025032803201713400_R13'} / J. Immunol. (1988)
{'first-page': '210', 'volume-title': 'Proc. Natl. Acad. Sci. USA', 'year': '1990', 'key': '2025032803201713400_R14'} / Proc. Natl. Acad. Sci. USA (1990)
{'first-page': '179', 'volume-title': 'Am. Rev. Respir. Dis.', 'year': '1990', 'key': '2025032803201713400_R15'} / Am. Rev. Respir. Dis. (1990)
{'first-page': '2935', 'volume-title': 'J. Biol. Chem.', 'year': '1996', 'key': '2025032803201713400_R16'} / J. Biol. Chem. (1996)
{'first-page': 'L888', 'volume-title': 'Am. J. Physiol.', 'year': '1997', 'key': '2025032803201713400_R17'} / Am. J. Physiol. (1997)
{'first-page': 'L51', 'volume-title': 'Am. J. Physiol.', 'year': '2000', 'key': '2025032803201713400_R18'} / Am. J. Physiol. (2000)
{'first-page': '167', 'volume-title': 'J. Leukocyte Biol.', 'year': '2002', 'key': '2025032803201713400_R19'} / J. Leukocyte Biol. (2002)
{'first-page': '311', 'volume-title': 'J. Clin. Invest.', 'year': '1994', 'key': '2025032803201713400_R20'} / J. Clin. Invest. (1994)
{'first-page': '3450', 'volume-title': 'Blood', 'year': '1996', 'key': '2025032803201713400_R21'} / Blood (1996)
{'first-page': 'L753', 'volume-title': 'Am. J. Physiol.', 'year': '1996', 'key': '2025032803201713400_R22'} / Am. J. Physiol. (1996)
{'first-page': 'L1156', 'volume-title': 'Am. J. Physiol.', 'year': '1997', 'key': '2025032803201713400_R23'} / Am. J. Physiol. (1997)
{'first-page': '449', 'volume-title': 'Biochem. J.', 'year': '2000', 'key': '2025032803201713400_R24'} / Biochem. J. (2000)
{'first-page': 'L563', 'volume-title': 'Am. J. Physiol.', 'year': '2001', 'key': '2025032803201713400_R25'} / Am. J. Physiol. (2001)
{'first-page': 'L606', 'volume-title': 'Am. J. Physiol.', 'year': '2005', 'key': '2025032803201713400_R26'} / Am. J. Physiol. (2005)
{'first-page': '6962', 'volume-title': 'J. Immunol.', 'year': '2006', 'key': '2025032803201713400_R27'} / J. Immunol. (2006)
{'first-page': '1295', 'volume-title': 'J. Immunol.', 'year': '1988', 'key': '2025032803201713400_R28'} / J. Immunol. (1988)
{'first-page': '1', 'volume-title': 'J. Immunol.', 'year': '1993', 'key': '2025032803201713400_R29'} / J. Immunol. (1993)
{'first-page': 'L958', 'volume-title': 'Am. J. Physiol.', 'year': '1998', 'key': '2025032803201713400_R30'} / Am. J. Physiol. (1998)
{'first-page': '107', 'volume-title': 'Adv. Opt. Electron Microsc.', 'year': '1982', 'key': '2025032803201713400_R31'} / Adv. Opt. Electron Microsc. (1982)
10.1093/oso/9780199632190.003.0009 / Electron Microscopy in Biology (1991)
{'first-page': '218', 'volume-title': 'Blood', 'year': '1990', 'key': '2025032803201713400_R33'} / Blood (1990)
{'first-page': '1640', 'volume-title': 'Infect. Immun.', 'year': '1999', 'key': '2025032803201713400_R34'} / Infect. Immun. (1999)
{'first-page': '233', 'volume-title': 'Diagn. Microbiol. Infect. Dis.', 'year': '1985', 'key': '2025032803201713400_R35'} / Diagn. Microbiol. Infect. Dis. (1985)
{'first-page': '633', 'volume-title': 'Biochem. J.', 'year': '2000', 'key': '2025032803201713400_R36'} / Biochem. J. (2000)
{'first-page': '2108', 'volume-title': 'J. Immunol.', 'year': '2000', 'key': '2025032803201713400_R37'} / J. Immunol. (2000)
{'first-page': '13724', 'volume-title': 'J. Biol. Chem.', 'year': '1996', 'key': '2025032803201713400_R38'} / J. Biol. Chem. (1996)
{'first-page': '1481', 'volume-title': 'Science', 'year': '1991', 'key': '2025032803201713400_R39'} / Science (1991)
{'first-page': '995', 'volume-title': 'Nat. Immunol.', 'year': '2005', 'key': '2025032803201713400_R40'} / Nat. Immunol. (2005)
{'first-page': '417', 'volume-title': 'Cell', 'year': '1982', 'key': '2025032803201713400_R41'} / Cell (1982)
{'first-page': '871', 'volume-title': 'Am. J. Respir. Cell Mol. Biol.', 'year': '2004', 'key': '2025032803201713400_R42'} / Am. J. Respir. Cell Mol. Biol. (2004)
{'first-page': '2448', 'volume-title': 'Proc. Natl. Acad. Sci. USA', 'year': '1996', 'key': '2025032803201713400_R43'} / Proc. Natl. Acad. Sci. USA (1996)
{'first-page': '59', 'volume-title': 'J. Leukocyte Biol.', 'year': '1994', 'key': '2025032803201713400_R44'} / J. Leukocyte Biol. (1994)
{'first-page': '230', 'volume-title': 'J. Leukocyte Biol.', 'year': '1992', 'key': '2025032803201713400_R45'} / J. Leukocyte Biol. (1992)
{'first-page': '3952', 'volume-title': 'J. Immunol.', 'year': '1995', 'key': '2025032803201713400_R46'} / J. Immunol. (1995)

Dates

Type	When
Created	11 years, 4 months ago (April 18, 2014, 7:18 p.m.)
Deposited	5 months ago (March 30, 2025, 6:44 p.m.)
Indexed	1 week, 4 days ago (Aug. 21, 2025, 2:02 p.m.)
Issued	18 years, 3 months ago (June 1, 2007)
Published	18 years, 3 months ago (June 1, 2007)
Published Online	18 years, 2 months ago (June 15, 2007)
Published Print	18 years, 2 months ago (June 15, 2007)

Funders 0

None

BibTeX

@article{Tecle_2007, title={Human Neutrophil Defensins Increase Neutrophil Uptake of Influenza A Virus and Bacteria and Modify Virus-Induced Respiratory Burst Responses}, volume={178}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.178.12.8046}, DOI={10.4049/jimmunol.178.12.8046}, number={12}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Tecle, Tesfaldet and White, Mitchell R and Gantz, Don and Crouch, Erika C and Hartshorn, Kevan L}, year={2007}, month=jun, pages={8046–8052} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        21
      ]
    ],
    "date-time": "2025-08-21T18:02:34Z",
    "timestamp": 1755799354281,
    "version": "3.40.3"
  },
  "reference-count": 46,
  "publisher": "Oxford University Press (OUP)",
  "issue": "12",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2007,
            6,
            1
          ]
        ],
        "date-time": "2007-06-01T00:00:00Z",
        "timestamp": 1180656000000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "https://academic.oup.com/pages/standard-publication-reuse-rights"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2007,
        6,
        15
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>Human neutrophil peptides (HNPs) are released from granules of neutrophils in response to various activating stimuli and they participate in the killing of bacteria and the stimulation of various inflammatory responses. HNPs also inhibit infectivity of enveloped viruses, including influenza A virus (IAV). In this study, we demonstrate that HNPs increase the uptake of IAV and bacteria by neutrophils. The dimeric HNPs also induced aggregation of IAV and bacterial particles, which may, in part, explain their ability to increase uptake. HNPs did not increase neutrophil respiratory burst responses to IAV. We have recently demonstrated direct interactions of HNPs with surfactant protein D (SP-D), another important effector of innate immunity and antimicrobial host defense. Although HNPs did not alter SP-D-dependent uptake of IAV, they counteracted the ability of SP-D to increase IAV-induced neutrophil H2O2 generation. Our studies reveal previously unappreciated functional effects of HNPs, expand our understanding of the antiviral properties of HNPs, and suggest important interactions between collectins and HNPs in the host response to viruses and bacteria.</jats:p>",
  "DOI": "10.4049/jimmunol.178.12.8046",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2014,
        4,
        18
      ]
    ],
    "date-time": "2014-04-18T23:18:11Z",
    "timestamp": 1397863091000
  },
  "page": "8046-8052",
  "source": "Crossref",
  "is-referenced-by-count": 87,
  "title": "Human Neutrophil Defensins Increase Neutrophil Uptake of Influenza A Virus and Bacteria and Modify Virus-Induced Respiratory Burst Responses",
  "prefix": "10.1093",
  "volume": "178",
  "author": [
    {
      "given": "Tesfaldet",
      "family": "Tecle",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Medicine, Boston University School of Medicine , Boston, MA 02118"
        }
      ]
    },
    {
      "given": "Mitchell R",
      "family": "White",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Medicine, Boston University School of Medicine , Boston, MA 02118"
        }
      ]
    },
    {
      "given": "Don",
      "family": "Gantz",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biophysics , Boston, MA 02118"
        }
      ]
    },
    {
      "given": "Erika C",
      "family": "Crouch",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Pathology and Immunology, Washington University School of Medicine , St. Louis, MO 63110"
        }
      ]
    },
    {
      "given": "Kevan L",
      "family": "Hartshorn",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Medicine, Boston University School of Medicine , Boston, MA 02118"
        }
      ]
    }
  ],
  "member": "286",
  "published-online": {
    "date-parts": [
      [
        2007,
        6,
        15
      ]
    ]
  },
  "reference": [
    {
      "first-page": "227",
      "volume-title": "J. Am. Med. Assoc.",
      "year": "2003",
      "key": "2025032803201713400_R1"
    },
    {
      "first-page": "303",
      "volume-title": "Microbiol. Rev.",
      "year": "1980",
      "key": "2025032803201713400_R2"
    },
    {
      "first-page": "5868",
      "volume-title": "J. Immunol.",
      "year": "2001",
      "key": "2025032803201713400_R3"
    },
    {
      "first-page": "2472",
      "volume-title": "J. Virol.",
      "year": "2000",
      "key": "2025032803201713400_R4"
    },
    {
      "first-page": "14933",
      "volume-title": "J. Virol.",
      "year": "2005",
      "key": "2025032803201713400_R5"
    },
    {
      "first-page": "425",
      "volume-title": "J. Gen. Virol.",
      "year": "1987",
      "key": "2025032803201713400_R6"
    },
    {
      "first-page": "499",
      "volume-title": "Options for the Control of Influenza",
      "year": "1996",
      "key": "2025032803201713400_R7"
    },
    {
      "first-page": "2040",
      "volume-title": "Clin. Infect. Dis.",
      "year": "2001",
      "key": "2025032803201713400_R8"
    },
    {
      "first-page": "765",
      "volume-title": "J. Clin. Invest.",
      "year": "2005",
      "key": "2025032803201713400_R9"
    },
    {
      "first-page": "1068",
      "volume-title": "J. Virol.",
      "year": "1986",
      "key": "2025032803201713400_R10"
    },
    {
      "first-page": "519",
      "volume-title": "Chest",
      "year": "2002",
      "key": "2025032803201713400_R11"
    },
    {
      "first-page": "L514",
      "volume-title": "Am. J. Physiol.",
      "year": "2004",
      "key": "2025032803201713400_R12"
    },
    {
      "first-page": "2686",
      "volume-title": "J. Immunol.",
      "year": "1988",
      "key": "2025032803201713400_R13"
    },
    {
      "first-page": "210",
      "volume-title": "Proc. Natl. Acad. Sci. USA",
      "year": "1990",
      "key": "2025032803201713400_R14"
    },
    {
      "first-page": "179",
      "volume-title": "Am. Rev. Respir. Dis.",
      "year": "1990",
      "key": "2025032803201713400_R15"
    },
    {
      "first-page": "2935",
      "volume-title": "J. Biol. Chem.",
      "year": "1996",
      "key": "2025032803201713400_R16"
    },
    {
      "first-page": "L888",
      "volume-title": "Am. J. Physiol.",
      "year": "1997",
      "key": "2025032803201713400_R17"
    },
    {
      "first-page": "L51",
      "volume-title": "Am. J. Physiol.",
      "year": "2000",
      "key": "2025032803201713400_R18"
    },
    {
      "first-page": "167",
      "volume-title": "J. Leukocyte Biol.",
      "year": "2002",
      "key": "2025032803201713400_R19"
    },
    {
      "first-page": "311",
      "volume-title": "J. Clin. Invest.",
      "year": "1994",
      "key": "2025032803201713400_R20"
    },
    {
      "first-page": "3450",
      "volume-title": "Blood",
      "year": "1996",
      "key": "2025032803201713400_R21"
    },
    {
      "first-page": "L753",
      "volume-title": "Am. J. Physiol.",
      "year": "1996",
      "key": "2025032803201713400_R22"
    },
    {
      "first-page": "L1156",
      "volume-title": "Am. J. Physiol.",
      "year": "1997",
      "key": "2025032803201713400_R23"
    },
    {
      "first-page": "449",
      "volume-title": "Biochem. J.",
      "year": "2000",
      "key": "2025032803201713400_R24"
    },
    {
      "first-page": "L563",
      "volume-title": "Am. J. Physiol.",
      "year": "2001",
      "key": "2025032803201713400_R25"
    },
    {
      "first-page": "L606",
      "volume-title": "Am. J. Physiol.",
      "year": "2005",
      "key": "2025032803201713400_R26"
    },
    {
      "first-page": "6962",
      "volume-title": "J. Immunol.",
      "year": "2006",
      "key": "2025032803201713400_R27"
    },
    {
      "first-page": "1295",
      "volume-title": "J. Immunol.",
      "year": "1988",
      "key": "2025032803201713400_R28"
    },
    {
      "first-page": "1",
      "volume-title": "J. Immunol.",
      "year": "1993",
      "key": "2025032803201713400_R29"
    },
    {
      "first-page": "L958",
      "volume-title": "Am. J. Physiol.",
      "year": "1998",
      "key": "2025032803201713400_R30"
    },
    {
      "first-page": "107",
      "volume-title": "Adv. Opt. Electron Microsc.",
      "year": "1982",
      "key": "2025032803201713400_R31"
    },
    {
      "key": "2025032803201713400_R32",
      "doi-asserted-by": "crossref",
      "first-page": "203",
      "DOI": "10.1093/oso/9780199632190.003.0009",
      "volume-title": "Electron Microscopy in Biology",
      "year": "1991"
    },
    {
      "first-page": "218",
      "volume-title": "Blood",
      "year": "1990",
      "key": "2025032803201713400_R33"
    },
    {
      "first-page": "1640",
      "volume-title": "Infect. Immun.",
      "year": "1999",
      "key": "2025032803201713400_R34"
    },
    {
      "first-page": "233",
      "volume-title": "Diagn. Microbiol. Infect. Dis.",
      "year": "1985",
      "key": "2025032803201713400_R35"
    },
    {
      "first-page": "633",
      "volume-title": "Biochem. J.",
      "year": "2000",
      "key": "2025032803201713400_R36"
    },
    {
      "first-page": "2108",
      "volume-title": "J. Immunol.",
      "year": "2000",
      "key": "2025032803201713400_R37"
    },
    {
      "first-page": "13724",
      "volume-title": "J. Biol. Chem.",
      "year": "1996",
      "key": "2025032803201713400_R38"
    },
    {
      "first-page": "1481",
      "volume-title": "Science",
      "year": "1991",
      "key": "2025032803201713400_R39"
    },
    {
      "first-page": "995",
      "volume-title": "Nat. Immunol.",
      "year": "2005",
      "key": "2025032803201713400_R40"
    },
    {
      "first-page": "417",
      "volume-title": "Cell",
      "year": "1982",
      "key": "2025032803201713400_R41"
    },
    {
      "first-page": "871",
      "volume-title": "Am. J. Respir. Cell Mol. Biol.",
      "year": "2004",
      "key": "2025032803201713400_R42"
    },
    {
      "first-page": "2448",
      "volume-title": "Proc. Natl. Acad. Sci. USA",
      "year": "1996",
      "key": "2025032803201713400_R43"
    },
    {
      "first-page": "59",
      "volume-title": "J. Leukocyte Biol.",
      "year": "1994",
      "key": "2025032803201713400_R44"
    },
    {
      "first-page": "230",
      "volume-title": "J. Leukocyte Biol.",
      "year": "1992",
      "key": "2025032803201713400_R45"
    },
    {
      "first-page": "3952",
      "volume-title": "J. Immunol.",
      "year": "1995",
      "key": "2025032803201713400_R46"
    }
  ],
  "container-title": "The Journal of Immunology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://academic.oup.com/jimmunol/article-pdf/178/12/8046/62653304/zim01207008046.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://academic.oup.com/jimmunol/article-pdf/178/12/8046/62653304/zim01207008046.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2025,
        3,
        30
      ]
    ],
    "date-time": "2025-03-30T22:44:13Z",
    "timestamp": 1743374653000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://academic.oup.com/jimmunol/article/178/12/8046/8022975"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2007,
        6
      ]
    ]
  },
  "references-count": 46,
  "journal-issue": {
    "issue": "12",
    "published-print": {
      "date-parts": [
        [
          2007,
          6,
          15
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.4049/jimmunol.178.12.8046",
  "relation": {},
  "ISSN": [
    "0022-1767",
    "1550-6606"
  ],
  "subject": [],
  "published-other": {
    "date-parts": [
      [
        2007,
        6
      ]
    ]
  },
  "published": {
    "date-parts": [
      [
        2007,
        6
      ]
    ]
  }
}