DOI: 10.4049/jimmunol.176.11.6770. The Mannose Receptor Mediates Uptake of Soluble but Not of Cell-Associated Antigen for Cross-Presentation

The Mannose Receptor Mediates Uptake of Soluble but Not of Cell-Associated Antigen for Cross-Presentation

10.4049/jimmunol.176.11.6770

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract The mannose receptor (MR) has been implicated in the recognition and clearance of microorganisms and serum glycoproteins. Its endocytic function has been studied extensively using macrophages, although it is expressed by a variety of cell types, including dendritic cells (DC). In this study, we investigated its role in Ag presentation by DC using MR−/− mice. Uptake of the model Ag, soluble OVA, by bone marrow-derived DC and in vitro activation of OVA-specific CD8 T cells (OT-I cells) strictly depended on the MR. In vivo, MR deficiency impaired endocytosis of soluble OVA by DC and concomitant OT-I cell activation. No alterations in the DC subtype composition in MR−/− mice were accountable. Uptake of cell-associated OVA was unaffected by MR deficiency, resulting in unchanged activation of OT-I cells. These findings demonstrate that DC use the MR for endocytosis of a particular Ag type intended for cross-presentation.

Bibliography

Burgdorf, S., Lukacs-Kornek, V., & Kurts, C. (2006). The Mannose Receptor Mediates Uptake of Soluble but Not of Cell-Associated Antigen for Cross-Presentation. The Journal of Immunology, 176(11), 6770â6776.

Authors 3

Sven Burgdorf (first)
Veronika Lukacs-Kornek (additional)
Christian Kurts (additional)

References 28 Referenced 232

Dates

Type	When
Created	11 years, 4 months ago (April 20, 2014, 7:47 p.m.)
Deposited	8 months ago (Jan. 2, 2025, 11:53 a.m.)
Indexed	6 days, 6 hours ago (Aug. 27, 2025, 11:33 a.m.)
Issued	19 years, 3 months ago (June 1, 2006)
Published	19 years, 3 months ago (June 1, 2006)
Published Print	19 years, 3 months ago (June 1, 2006)

Funders 0

None

BibTeX

@article{Burgdorf_2006, title={The Mannose Receptor Mediates Uptake of Soluble but Not of Cell-Associated Antigen for Cross-Presentation}, volume={176}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.176.11.6770}, DOI={10.4049/jimmunol.176.11.6770}, number={11}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Burgdorf, Sven and Lukacs-Kornek, Veronika and Kurts, Christian}, year={2006}, month=jun, pages={6770–6776} }

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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>The mannose receptor (MR) has been implicated in the recognition and clearance of microorganisms and serum glycoproteins. Its endocytic function has been studied extensively using macrophages, although it is expressed by a variety of cell types, including dendritic cells (DC). In this study, we investigated its role in Ag presentation by DC using MR\u2212/\u2212 mice. Uptake of the model Ag, soluble OVA, by bone marrow-derived DC and in vitro activation of OVA-specific CD8 T cells (OT-I cells) strictly depended on the MR. In vivo, MR deficiency impaired endocytosis of soluble OVA by DC and concomitant OT-I cell activation. No alterations in the DC subtype composition in MR\u2212/\u2212 mice were accountable. Uptake of cell-associated OVA was unaffected by MR deficiency, resulting in unchanged activation of OT-I cells. These findings demonstrate that DC use the MR for endocytosis of a particular Ag type intended for cross-presentation.</jats:p>",
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