DOI: 10.4049/jimmunol.173.3.1658. Differential Roles for Wiskott-Aldrich Syndrome Protein in Immune Synapse Formation and IL-2 Production [Penn]

Differential Roles for Wiskott-Aldrich Syndrome Protein in Immune Synapse Formation and IL-2 Production

10.4049/jimmunol.173.3.1658

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Wiskott-Aldrich syndrome protein (WASP)-deficient T cells exhibit defects in IL-2 production that are widely believed to stem from primary defects in actin remodeling and immune synapse formation. Surprisingly, however, we find that WASP-deficient T cells responding to Ag-specific APCs polymerize actin and organize talin and PKCθ normally, forming an immune synapse that is stable for at least 3 h. At low doses of peptide, WASP-deficient T cells show less efficient talin and PKCθ polarization. Thus, although WASP may facilitate immune synapse formation at low peptide concentrations, WASP is not required for this process. Defects in IL-2 production are observed even under conditions in which immune synapse formation proceeds normally, suggesting that the role of WASP in regulating IL-2 production is independent of its role in immune synapse formation.

Bibliography

Cannon, J. L., & Burkhardt, J. K. (2004). Differential Roles for Wiskott-Aldrich Syndrome Protein in Immune Synapse Formation and IL-2 Production. The Journal of Immunology, 173(3), 1658â1662.

Authors 2

Judy L. Cannon (first)
Janis K. Burkhardt (additional)

References 34 Referenced 99

Dates

Type	When
Created	11 years, 4 months ago (April 22, 2014, 11:38 p.m.)
Deposited	8 months ago (Jan. 2, 2025, 12:25 p.m.)
Indexed	1 week, 3 days ago (Aug. 27, 2025, 11:53 a.m.)
Issued	21 years, 1 month ago (Aug. 1, 2004)
Published	21 years, 1 month ago (Aug. 1, 2004)
Published Print	21 years, 1 month ago (Aug. 1, 2004)

Funders 0

None

BibTeX

@article{Cannon_2004, title={Differential Roles for Wiskott-Aldrich Syndrome Protein in Immune Synapse Formation and IL-2 Production}, volume={173}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.173.3.1658}, DOI={10.4049/jimmunol.173.3.1658}, number={3}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Cannon, Judy L. and Burkhardt, Janis K.}, year={2004}, month=aug, pages={1658–1662} }

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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>Wiskott-Aldrich syndrome protein (WASP)-deficient T cells exhibit defects in IL-2 production that are widely believed to stem from primary defects in actin remodeling and immune synapse formation. Surprisingly, however, we find that WASP-deficient T cells responding to Ag-specific APCs polymerize actin and organize talin and PKC\u03b8 normally, forming an immune synapse that is stable for at least 3 h. At low doses of peptide, WASP-deficient T cells show less efficient talin and PKC\u03b8 polarization. Thus, although WASP may facilitate immune synapse formation at low peptide concentrations, WASP is not required for this process. Defects in IL-2 production are observed even under conditions in which immune synapse formation proceeds normally, suggesting that the role of WASP in regulating IL-2 production is independent of its role in immune synapse formation.</jats:p>",
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