DOI: 10.4049/jimmunol.173.10.6189. PIASy-Deficient Mice Display Modest Defects in IFN and Wnt Signaling

PIASy-Deficient Mice Display Modest Defects in IFN and Wnt Signaling
10.4049/jimmunol.173.10.6189
Crossref journal-article
Oxford University Press (OUP)
The Journal of Immunology (286)
Abstract
Abstract Protein inhibitors of activated STATs (PIAS) represent a small family of nuclear proteins that modulate the activity of many transcription factors and act as E3 ligases for covalent modification of proteins with the small ubiquitin-like modifier (SUMO). In particular, PIASy has been shown to inhibit the activation of gene expression by the IFN-responsive transcription factor STAT1 and the Wnt-responsive transcription factor LEF1. To assess the function of PIASy in vivo, we generated and analyzed mice carrying a targeted mutation of the Piasy gene. We find that homozygous mutant mice have no obvious morphological defects and have a normal distribution of lymphocyte populations. Molecular analysis of signaling in response to IFN-γ and Wnt agonists revealed a modest reduction in the activation of endogenous and transfected target genes. Two-dimensional analysis of total proteins and SUMO-modified proteins in transformed pre-B cells showed no significant differences between wild-type mice and homozygous mutant mice. Taken together, our data indicate that PIASy has a modest effect on cytokine and Wnt signaling, suggesting a redundancy with other members of the family of PIAS proteins.
Bibliography
Roth, W., Sustmann, C., Kieslinger, M., Gilmozzi, A., Irmer, D., Kremmer, E., Turck, C., & Grosschedl, R. (2004). PIASy-Deficient Mice Display Modest Defects in IFN and Wnt Signaling. The Journal of Immunology, 173(10), 6189â6199.
Authors 8
Wera Roth (first)
Claudio Sustmann (additional)
Matthias Kieslinger (additional)
Andrea Gilmozzi (additional)
Denis Irmer (additional)
Elisabeth Kremmer (additional)
Chris Turck (additional)
Rudolf Grosschedl (additional)
References 76 Referenced 72
Dates
Type	When
Created	11 years, 4 months ago (April 20, 2014, 8:57 p.m.)
Deposited	8 months ago (Jan. 2, 2025, 1:44 p.m.)
Indexed	4 months, 3 weeks ago (April 10, 2025, 7:12 a.m.)
Issued	20 years, 9 months ago (Nov. 15, 2004)
Published	20 years, 9 months ago (Nov. 15, 2004)
Published Print	20 years, 9 months ago (Nov. 15, 2004)
Funders 0
None
BibTeX
@article{Roth_2004, title={PIASy-Deficient Mice Display Modest Defects in IFN and Wnt Signaling}, volume={173}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.173.10.6189}, DOI={10.4049/jimmunol.173.10.6189}, number={10}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Roth, Wera and Sustmann, Claudio and Kieslinger, Matthias and Gilmozzi, Andrea and Irmer, Denis and Kremmer, Elisabeth and Turck, Chris and Grosschedl, Rudolf}, year={2004}, month=nov, pages={6189–6199} }
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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>Protein inhibitors of activated STATs (PIAS) represent a small family of nuclear proteins that modulate the activity of many transcription factors and act as E3 ligases for covalent modification of proteins with the small ubiquitin-like modifier (SUMO). In particular, PIASy has been shown to inhibit the activation of gene expression by the IFN-responsive transcription factor STAT1 and the Wnt-responsive transcription factor LEF1. To assess the function of PIASy in vivo, we generated and analyzed mice carrying a targeted mutation of the Piasy gene. We find that homozygous mutant mice have no obvious morphological defects and have a normal distribution of lymphocyte populations. Molecular analysis of signaling in response to IFN-\u03b3 and Wnt agonists revealed a modest reduction in the activation of endogenous and transfected target genes. Two-dimensional analysis of total proteins and SUMO-modified proteins in transformed pre-B cells showed no significant differences between wild-type mice and homozygous mutant mice. Taken together, our data indicate that PIASy has a modest effect on cytokine and Wnt signaling, suggesting a redundancy with other members of the family of PIAS proteins.</jats:p>",
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