DOI: 10.4049/jimmunol.171.12.6795. Surface Translocation of Pactolus Is Induced by Cell Activation and Death, but Is Not Required for Neutrophil Migration and Function

Surface Translocation of Pactolus Is Induced by Cell Activation and Death, but Is Not Required for Neutrophil Migration and Function

10.4049/jimmunol.171.12.6795

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Pactolus is a cell surface protein expressed by murine neutrophils. Pactolus is similar to the β integrins, except it lacks a functional metal ion-dependent adhesion site domain and is expressed without an α-chain partner. The majority of the Pactolus protein is held within the cell in dense granules in a highly glycosylated form. This intracellular form of Pactolus can be released to the cell surface following inflammatory activation or ligation of Pactolus on the cell surface. In addition, intracellular Pactolus translocates to the neutrophil surface following induction of apoptosis. Neutrophil activation studies suggest that Pactolus does not serve as an activating or phagocytic receptor for the neutrophil. To further define the function of Pactolus, a Pactolus-null mouse was generated. Pactolus-deficient animals mature appropriately and possess normal numbers of neutrophils, display appropriate migration into sites of inflammation, and combat introduced infections efficiently. These data suggest that Pactolus does not function as a neutrophil phagocytic or adhesion receptor, but may instead serve as a sugar-bearing ligand for lectin recognition by other cells.

Bibliography

Garrison, S., Hojgaard, A., Margraf, R., Weis, J. J., & Weis, J. H. (2003). Surface Translocation of Pactolus Is Induced by Cell Activation and Death, but Is Not Required for Neutrophil Migration and Function. The Journal of Immunology, 171(12), 6795â6806.

Authors 5

Sean Garrison (first)
Andrias Hojgaard (additional)
Rebecca Margraf (additional)
Janis J. Weis (additional)
John H. Weis (additional)

References 43 Referenced 7

Dates

Type	When
Created	11 years, 4 months ago (April 20, 2014, 9:30 p.m.)
Deposited	8 months ago (Jan. 2, 2025, 1:38 p.m.)
Indexed	8 months ago (Jan. 3, 2025, 12:35 a.m.)
Issued	21 years, 8 months ago (Dec. 15, 2003)
Published	21 years, 8 months ago (Dec. 15, 2003)
Published Print	21 years, 8 months ago (Dec. 15, 2003)

Funders 0

None

BibTeX

@article{Garrison_2003, title={Surface Translocation of Pactolus Is Induced by Cell Activation and Death, but Is Not Required for Neutrophil Migration and Function}, volume={171}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.171.12.6795}, DOI={10.4049/jimmunol.171.12.6795}, number={12}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Garrison, Sean and Hojgaard, Andrias and Margraf, Rebecca and Weis, Janis J. and Weis, John H.}, year={2003}, month=dec, pages={6795–6806} }

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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>Pactolus is a cell surface protein expressed by murine neutrophils. Pactolus is similar to the \u03b2 integrins, except it lacks a functional metal ion-dependent adhesion site domain and is expressed without an \u03b1-chain partner. The majority of the Pactolus protein is held within the cell in dense granules in a highly glycosylated form. This intracellular form of Pactolus can be released to the cell surface following inflammatory activation or ligation of Pactolus on the cell surface. In addition, intracellular Pactolus translocates to the neutrophil surface following induction of apoptosis. Neutrophil activation studies suggest that Pactolus does not serve as an activating or phagocytic receptor for the neutrophil. To further define the function of Pactolus, a Pactolus-null mouse was generated. Pactolus-deficient animals mature appropriately and possess normal numbers of neutrophils, display appropriate migration into sites of inflammation, and combat introduced infections efficiently. These data suggest that Pactolus does not function as a neutrophil phagocytic or adhesion receptor, but may instead serve as a sugar-bearing ligand for lectin recognition by other cells.</jats:p>",
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    "date-time": "2025-01-02T18:38:24Z",
    "timestamp": 1735843104000
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  "resource": {
    "primary": {
      "URL": "https://journals.aai.org/jimmunol/article/171/12/6795/72186/Surface-Translocation-of-Pactolus-Is-Induced-by"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
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        2003,
        12,
        15
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  "URL": "http://dx.doi.org/10.4049/jimmunol.171.12.6795",
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  "ISSN": [
    "0022-1767",
    "1550-6606"
  ],
  "subject": [],
  "published": {
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      [
        2003,
        12,
        15
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    ]
  }
}