DOI: 10.4049/jimmunol.169.8.4108. Cutting Edge: A Natural P451L Mutation in the Cytoplasmic Domain Impairs the Function of the Mouse P2X7 Receptor

Cutting Edge: A Natural P451L Mutation in the Cytoplasmic Domain Impairs the Function of the Mouse P2X7 Receptor

10.4049/jimmunol.169.8.4108

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

AbstractThe P2X7 receptor (P2X7R) is an ATP-gated channel that mediates apoptosis of cells of the immune system. The capacity of P2X7R to form large pores depends on its large cytoplasmic tail, which harbors a putative TNFR-related death domain. Previous transfection studies indicated that mouse P2X7R forms pores much less efficiently than its counterparts from humans and rats. In this study, we demonstrate that an allelic mutation (P451L) in the predicted death domain of P2X7R confers a drastically reduced sensitivity to ATP-induced pore formation in cells from some commonly used strains of mice, i.e., C57BL/6 and DBA/2. In contrast, most other strains of mice, including strains derived from wild mice, carry P451 at this position as do rats and humans. The effects of the P451L mutation resemble those of the E496A mutation in human P2X7R. These P2X7R mutants may provide useful tools to decipher the molecular mechanisms leading to pore formation.

Bibliography

Adriouch, S., Dox, C., Welge, V., Seman, M., Koch-Nolte, F., & Haag, F. (2002). Cutting Edge: A Natural P451L Mutation in the Cytoplasmic Domain Impairs the Function of the Mouse P2X7 Receptor. The Journal of Immunology, 169(8), 4108â4112.

Authors 6

Sahil Adriouch (first)
Claudia Dox (additional)
Vivienne Welge (additional)
Michel Seman (additional)
Friedrich Koch-Nolte (additional)
Friedrich Haag (additional)

References 35 Referenced 162

Dates

Type	When
Created	11 years, 4 months ago (April 20, 2014, 8:03 p.m.)
Deposited	4 months ago (May 2, 2025, 9:24 a.m.)
Indexed	2 months, 3 weeks ago (June 10, 2025, 8:48 a.m.)
Issued	22 years, 10 months ago (Oct. 15, 2002)
Published	22 years, 10 months ago (Oct. 15, 2002)
Published Print	22 years, 10 months ago (Oct. 15, 2002)

Funders 0

None

BibTeX

@article{Adriouch_2002, title={Cutting Edge: A Natural P451L Mutation in the Cytoplasmic Domain Impairs the Function of the Mouse P2X7 Receptor}, volume={169}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.169.8.4108}, DOI={10.4049/jimmunol.169.8.4108}, number={8}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Adriouch, Sahil and Dox, Claudia and Welge, Vivienne and Seman, Michel and Koch-Nolte, Friedrich and Haag, Friedrich}, year={2002}, month=oct, pages={4108–4112} }

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