DOI: 10.4049/jimmunol.169.6.3267. Surfactant Protein D Binds Selectively to<i>Klebsiella pneumoniae</i>Lipopolysaccharides Containing Mannose-Rich O-Antigens

Surfactant Protein D Binds Selectively toKlebsiella pneumoniaeLipopolysaccharides Containing Mannose-Rich O-Antigens

10.4049/jimmunol.169.6.3267

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

AbstractSurfactant protein D (SP-D) plays important roles in the regulation of innate immune responses in the lung. We have previously shown that SP-D can agglutinate and enhance the macrophage-dependent killing of specific unencapsulated phase variants of Klebsiella pneumoniae. In the present studies, we used 16 clinical isolates of Klebsiella representing four O-serotypes and examined the interaction of SP-D with their isolated LPSs. Although SP-D bound to the core oligosaccharide of rough LPS from all isolates, it selectively bound to smooth forms of LPS expressed by O-serotypes with mannose-rich repeating units in their O-polysaccharides. SP-D was more potent in agglutinating unencapsulated phase variants of O-serotypes expressing these SP-D “reactive” O-polysaccharides, and more effectively inhibited the adhesion of these serotypes to lung epithelial cells. This novel anti-adhesion activity required the multimerization of trimeric SP-D subunits (dodecamers). Klebsiella serotypes expressing “nonreactive” LPS O-Ags were isolated at a significantly higher frequency from patients with K. pneumoniae. Our findings suggest that SP-D plays important roles in the clearance of opportunistic Gram-negative bacteria and contributes to known serotypic differences in the pathogenicity of Klebsiella through specific interactions with O-polysaccharides.

Bibliography

Sahly, H., Ofek, I., Podschun, R., Brade, H., He, Y., Ullmann, U., & Crouch, E. (2002). Surfactant Protein D Binds Selectively toKlebsiella pneumoniaeLipopolysaccharides Containing Mannose-Rich O-Antigens. The Journal of Immunology, 169(6), 3267â3274.

Authors 7

Hany Sahly (first)
Itzhak Ofek (additional)
Rainer Podschun (additional)
Helmut Brade (additional)
Yanchun He (additional)
Uwe Ullmann (additional)
Erika Crouch (additional)

References 53 Referenced 46

Dates

Type	When
Created	11 years, 4 months ago (April 20, 2014, 9:59 p.m.)
Deposited	7 months, 3 weeks ago (Jan. 2, 2025, 12:23 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:35 p.m.)
Issued	22 years, 11 months ago (Sept. 15, 2002)
Published	22 years, 11 months ago (Sept. 15, 2002)
Published Print	22 years, 11 months ago (Sept. 15, 2002)

Funders 0

None

BibTeX

@article{Sahly_2002, title={Surfactant Protein D Binds Selectively toKlebsiella pneumoniaeLipopolysaccharides Containing Mannose-Rich O-Antigens}, volume={169}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.169.6.3267}, DOI={10.4049/jimmunol.169.6.3267}, number={6}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Sahly, Hany and Ofek, Itzhak and Podschun, Rainer and Brade, Helmut and He, Yanchun and Ullmann, Uwe and Crouch, Erika}, year={2002}, month=sep, pages={3267–3274} }

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  "abstract": "<jats:title>Abstract</jats:title><jats:p>Surfactant protein D (SP-D) plays important roles in the regulation of innate immune responses in the lung. We have previously shown that SP-D can agglutinate and enhance the macrophage-dependent killing of specific unencapsulated phase variants of Klebsiella pneumoniae. In the present studies, we used 16 clinical isolates of Klebsiella representing four O-serotypes and examined the interaction of SP-D with their isolated LPSs. Although SP-D bound to the core oligosaccharide of rough LPS from all isolates, it selectively bound to smooth forms of LPS expressed by O-serotypes with mannose-rich repeating units in their O-polysaccharides. SP-D was more potent in agglutinating unencapsulated phase variants of O-serotypes expressing these SP-D \u201creactive\u201d O-polysaccharides, and more effectively inhibited the adhesion of these serotypes to lung epithelial cells. This novel anti-adhesion activity required the multimerization of trimeric SP-D subunits (dodecamers). Klebsiella serotypes expressing \u201cnonreactive\u201d LPS O-Ags were isolated at a significantly higher frequency from patients with K. pneumoniae. Our findings suggest that SP-D plays important roles in the clearance of opportunistic Gram-negative bacteria and contributes to known serotypic differences in the pathogenicity of Klebsiella through specific interactions with O-polysaccharides.</jats:p>",
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