DOI: 10.4049/jimmunol.167.9.5273. Immunization of Mice Against West Nile Virus with Recombinant Envelope Protein

Immunization of Mice Against West Nile Virus with Recombinant Envelope Protein

10.4049/jimmunol.167.9.5273

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract West Nile (WN) virus is a mosquito-borne flavivirus that emerged in the United States in 1999 and can cause fatal encephalitis. Envelope (E) protein cDNA from a WN virus isolate recovered from Culex pipiens in Connecticut was expressed in Escherichia coli. The recombinant E protein was purified and used as Ag in immunoblot assays and immunization experiments. Patients with WN virus infection had Abs that recognized the recombinant E protein. C3H/HeN mice immunized with E protein developed E protein Abs and were protected from infection with WN virus. Passive administration of E protein antisera was also sufficient to afford immunity. E protein is a candidate vaccine to prevent WN virus infection.

Authors 6

Tian Wang (first)
John F. Anderson (additional)
Louis A. Magnarelli (additional)
Susan J. Wong (additional)
Raymond A. Koski (additional)
Erol Fikrig (additional)

References 34 Referenced 82

Dates

Type	When
Created	11 years, 4 months ago (April 20, 2014, 11:19 p.m.)
Deposited	8 months ago (Jan. 2, 2025, 2:04 p.m.)
Indexed	5 months ago (March 30, 2025, 7:03 p.m.)
Issued	23 years, 10 months ago (Nov. 1, 2001)
Published	23 years, 10 months ago (Nov. 1, 2001)
Published Print	23 years, 10 months ago (Nov. 1, 2001)

Funders 0

None

BibTeX

@article{Wang_2001, title={Immunization of Mice Against West Nile Virus with Recombinant Envelope Protein}, volume={167}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.167.9.5273}, DOI={10.4049/jimmunol.167.9.5273}, number={9}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Wang, Tian and Anderson, John F. and Magnarelli, Louis A. and Wong, Susan J. and Koski, Raymond A. and Fikrig, Erol}, year={2001}, month=nov, pages={5273–5277} }

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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>West Nile (WN) virus is a mosquito-borne flavivirus that emerged in the United States in 1999 and can cause fatal encephalitis. Envelope (E) protein cDNA from a WN virus isolate recovered from Culex pipiens in Connecticut was expressed in Escherichia coli. The recombinant E protein was purified and used as Ag in immunoblot assays and immunization experiments. Patients with WN virus infection had Abs that recognized the recombinant E protein. C3H/HeN mice immunized with E protein developed E protein Abs and were protected from infection with WN virus. Passive administration of E protein antisera was also sufficient to afford immunity. E protein is a candidate vaccine to prevent WN virus infection.</jats:p>",
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