DOI: 10.4049/jimmunol.167.2.741. The Dendritic Cell Populations of Mouse Lymph Nodes

The Dendritic Cell Populations of Mouse Lymph Nodes

10.4049/jimmunol.167.2.741

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

AbstractThe dendritic cells (DC) of mouse lymph nodes (LN) were isolated, analyzed for surface markers, and compared with those of spleen. Low to moderate staining of LN DC for CD4 and low staining for CD8 was shown to be attributable to pickup of these markers from T cells. Excluding this artifact, five LN DC subsets could be delineated. They included the three populations found in spleen (CD4+8−DEC-205−, CD4−8−DEC-205−, CD4−8+DEC-205+), although the CD4-expressing DC were of low incidence. LN DC included two additional populations, characterized by relatively low expression of CD8 but moderate or high expression of DEC-205. Both appeared among the DC migrating out of skin into LN, but only one was restricted to skin-draining LN and was identified as the mature form of epidermal Langerhans cells (LC). The putative LC-derived DC displayed the following properties: large size; high levels of class II MHC, which persisted to some extent even in CIITA null mice; expression of very high levels of DEC-205 and of CD40; expression of many myeloid surface markers; and no expression of CD4 and only low to moderate expression of CD8. The putative LC-derived DC among skin emigrants and in LN also showed strong intracellular staining of langerin.

Bibliography

Henri, S., Vremec, D., Kamath, A., Waithman, J., Williams, S., Benoist, C., Burnham, K., Saeland, S., Handman, E., & Shortman, K. (2001). The Dendritic Cell Populations of Mouse Lymph Nodes. The Journal of Immunology, 167(2), 741â748.

Authors 10

Sandrine Henri (first)
David Vremec (additional)
Arun Kamath (additional)
Jason Waithman (additional)
Stuart Williams (additional)
Christophe Benoist (additional)
Kim Burnham (additional)
Sem Saeland (additional)
Emanuela Handman (additional)
Ken Shortman (additional)

References 33 Referenced 344

Dates

Type	When
Created	11 years, 4 months ago (April 21, 2014, 10:17 p.m.)
Deposited	7 months, 3 weeks ago (Jan. 2, 2025, 11:45 a.m.)
Indexed	1 month, 1 week ago (July 16, 2025, 7:53 a.m.)
Issued	24 years, 1 month ago (July 15, 2001)
Published	24 years, 1 month ago (July 15, 2001)
Published Print	24 years, 1 month ago (July 15, 2001)

Funders 0

None

BibTeX

@article{Henri_2001, title={The Dendritic Cell Populations of Mouse Lymph Nodes}, volume={167}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.167.2.741}, DOI={10.4049/jimmunol.167.2.741}, number={2}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Henri, Sandrine and Vremec, David and Kamath, Arun and Waithman, Jason and Williams, Stuart and Benoist, Christophe and Burnham, Kim and Saeland, Sem and Handman, Emanuela and Shortman, Ken}, year={2001}, month=jul, pages={741–748} }

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  "abstract": "<jats:title>Abstract</jats:title><jats:p>The dendritic cells (DC) of mouse lymph nodes (LN) were isolated, analyzed for surface markers, and compared with those of spleen. Low to moderate staining of LN DC for CD4 and low staining for CD8 was shown to be attributable to pickup of these markers from T cells. Excluding this artifact, five LN DC subsets could be delineated. They included the three populations found in spleen (CD4+8\u2212DEC-205\u2212, CD4\u22128\u2212DEC-205\u2212, CD4\u22128+DEC-205+), although the CD4-expressing DC were of low incidence. LN DC included two additional populations, characterized by relatively low expression of CD8 but moderate or high expression of DEC-205. Both appeared among the DC migrating out of skin into LN, but only one was restricted to skin-draining LN and was identified as the mature form of epidermal Langerhans cells (LC). The putative LC-derived DC displayed the following properties: large size; high levels of class II MHC, which persisted to some extent even in CIITA null mice; expression of very high levels of DEC-205 and of CD40; expression of many myeloid surface markers; and no expression of CD4 and only low to moderate expression of CD8. The putative LC-derived DC among skin emigrants and in LN also showed strong intracellular staining of langerin.</jats:p>",
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