DOI: 10.4049/jimmunol.165.9.5084. TNFR-Associated Factor Family Protein Expression in Normal Tissues and Lymphoid Malignancies

TNFR-Associated Factor Family Protein Expression in Normal Tissues and Lymphoid Malignancies

10.4049/jimmunol.165.9.5084

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract TNFR-associated factors (TRAFs) constitute a family of adapter proteins that associate with particular TNF family receptors. Humans and mice contain six TRAF genes, but little is known about their in vivo expression at the single cell level. The in vivo locations of TRAF1, TRAF2, TRAF5, and TRAF6 were determined in human and mouse tissues by immunohistochemistry. Striking diversity was observed in the patterns of immunostaining obtained for each TRAF family protein, suggesting their expression is independently regulated in a cell type-specific manner. Dynamic regulation of TRAFs was observed in cultured PBLs, where anti-CD3 Abs, mitogenic lectins, and ILs induced marked increases in the steady-state levels of TRAF1, TRAF2, TRAF5, and TRAF6. TRAF1 was also highly inducible by CD40 ligand in cultured germinal center B cells, whereas TRAF2, TRAF3, TRAF5, and TRAF6 were relatively unchanged. Analysis of 83 established human tumor cell lines by semiquantitative immunoblotting methods revealed tendencies of certain cancer types to express particular TRAFs. For example, expression of TRAF1 was highly restricted, with B cell lymphomas consistently expressing this TRAF family member. Consistent with results from tumor cell lines, immunohistochemical analysis of 232 non-Hodgkin lymphomas revealed TRAF1 overexpression in 112 (48%) cases. TRAF1 protein levels were also elevated in circulating B cell chronic lymphocytic leukemia specimens (n = 49) compared with normal peripheral blood B cells (p = 0.01), as determined by immunoblotting. These findings contribute to an improved understanding of the cell-specific roles of TRAFs in normal tissues and provide evidence of altered TRAF1 expression in lymphoid malignancies.

Bibliography

Zapata, J. M., Krajewska, M., Krajewski, S., Kitada, S., Welsh, K., Monks, A., McCloskey, N., Gordon, J., Kipps, T. J., Gascoyne, R. D., Shabaik, A., & Reed, J. C. (2000). TNFR-Associated Factor Family Protein Expression in Normal Tissues and Lymphoid Malignancies. The Journal of Immunology, 165(9), 5084â5096.

Authors 12

Juan M. Zapata (first)
Maryla Krajewska (additional)
Stanislaw Krajewski (additional)
Shinichi Kitada (additional)
Kate Welsh (additional)
Anne Monks (additional)
Natalie McCloskey (additional)
John Gordon (additional)
Thomas J. Kipps (additional)
Randy D. Gascoyne (additional)
Ahmed Shabaik (additional)
John C. Reed (additional)

References 63 Referenced 116

Dates

Type	When
Created	11 years, 4 months ago (April 20, 2014, 10:59 p.m.)
Deposited	8 months ago (Jan. 2, 2025, 12:33 p.m.)
Indexed	5 months ago (March 30, 2025, 6:09 a.m.)
Issued	24 years, 10 months ago (Nov. 1, 2000)
Published	24 years, 10 months ago (Nov. 1, 2000)
Published Print	24 years, 10 months ago (Nov. 1, 2000)

Funders 0

None

BibTeX

@article{Zapata_2000, title={TNFR-Associated Factor Family Protein Expression in Normal Tissues and Lymphoid Malignancies}, volume={165}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.165.9.5084}, DOI={10.4049/jimmunol.165.9.5084}, number={9}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Zapata, Juan M. and Krajewska, Maryla and Krajewski, Stanislaw and Kitada, Shinichi and Welsh, Kate and Monks, Anne and McCloskey, Natalie and Gordon, John and Kipps, Thomas J. and Gascoyne, Randy D. and Shabaik, Ahmed and Reed, John C.}, year={2000}, month=nov, pages={5084–5096} }

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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>TNFR-associated factors (TRAFs) constitute a family of adapter proteins that associate with particular TNF family receptors. Humans and mice contain six TRAF genes, but little is known about their in vivo expression at the single cell level. The in vivo locations of TRAF1, TRAF2, TRAF5, and TRAF6 were determined in human and mouse tissues by immunohistochemistry. Striking diversity was observed in the patterns of immunostaining obtained for each TRAF family protein, suggesting their expression is independently regulated in a cell type-specific manner. Dynamic regulation of TRAFs was observed in cultured PBLs, where anti-CD3 Abs, mitogenic lectins, and ILs induced marked increases in the steady-state levels of TRAF1, TRAF2, TRAF5, and TRAF6. TRAF1 was also highly inducible by CD40 ligand in cultured germinal center B cells, whereas TRAF2, TRAF3, TRAF5, and TRAF6 were relatively unchanged. Analysis of 83 established human tumor cell lines by semiquantitative immunoblotting methods revealed tendencies of certain cancer types to express particular TRAFs. For example, expression of TRAF1 was highly restricted, with B cell lymphomas consistently expressing this TRAF family member. Consistent with results from tumor cell lines, immunohistochemical analysis of 232 non-Hodgkin lymphomas revealed TRAF1 overexpression in 112 (48%) cases. TRAF1 protein levels were also elevated in circulating B cell chronic lymphocytic leukemia specimens (n = 49) compared with normal peripheral blood B cells (p = 0.01), as determined by immunoblotting. These findings contribute to an improved understanding of the cell-specific roles of TRAFs in normal tissues and provide evidence of altered TRAF1 expression in lymphoid malignancies.</jats:p>",
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      "unstructured": "Declercq, W.,  G. Denecker,  W. Fiers,  P. Vandenabeele.  1998. Cooperation of both TNF receptors in inducing apoptosis: involvement of the TNF receptor-associated factor binding domain of the TNF receptor p75. J. Immunol.\u2008161: 390",
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      "unstructured": "Lee, S. Y.,  D. R. Kaufman,  A. L. Mora,  A. Santana,  M. Boothby,  Y. Choi.  1998. Stimulus-dependent synergism of the anti-apoptotic tumor necrosis factor receptor-associated factor 2 (TRAF2) and nuclear factor \u03baB pathways. J. Exp. Med.\u2008188: 1381",
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      "DOI": "10.1126/science.274.5289.990"
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