DOI: 10.4049/jimmunol.165.3.1381. Functional Involvement of E-Cadherin in TGF-β1-Induced Cell Cluster Formation of In Vitro Developing Human Langerhans-Type Dendritic Cells

Functional Involvement of E-Cadherin in TGF-β1-Induced Cell Cluster Formation of In Vitro Developing Human Langerhans-Type Dendritic Cells

10.4049/jimmunol.165.3.1381

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

AbstractEpithelial Langerhans cells (LC) represent immature dendritic cells that require TGF-β1 stimulation for their development. Little is known about the mechanisms regulating LC generation from their precursor cells. We demonstrate here that LC development from human CD34+ hemopoietic progenitor cells in response to TGF-β1 costimulation (basic cytokine combination GM-CSF plus TNF-α, stem cell factor, and Flt3 ligand) is associated with pronounced cell cluster formation of developing LC precursor cells. This cell-clustering phenomenon requires hemopoietic progenitor cell differentiation, since it is first seen on day 4 after culture initiation of CD34+ cells. Cell cluster formation morphologically indicates progenitor cell development along the LC pathway, because parallel cultures set up in the absence of exogenous TGF-β1 fail to form cell clusters and predominantly give rise to monocyte, but not LC, development (CD1a−, lysozyme+, CD14+). TGF-β1 costimulation of CD34+ cells induces neoexpression of the homophilic adhesion molecule E-cadherin in the absence of the E-cadherin heteroligand CD103. Addition of anti-E-cadherin mAb or mAbs to any of the constitutively expressed adhesion molecule (CD99, CD31, LFA-1, or CD18) to TGF-β1-supplemented progenitor cell cultures inhibits LC precursor cell cluster formation, and this effect is, with the exception of anti-E-cadherin mAb, associated with inhibition of LC generation. Addition of anti-E-cadherin mAb to the culture allows cell cluster-independent generation of LC from CD34+ cells. Thus, functional E-cadherin expression and homotypic cell cluster formation represent a regular response of LC precursor cells to TGF-β1 stimulation, and cytoadhesive interactions may modulate LC differentiation from hemopoietic progenitor cells.

Bibliography

Riedl, E., StoÌckl, J., Majdic, O., Scheinecker, C., Rappersberger, K., Knapp, W., & Strobl, H. (2000). Functional Involvement of E-Cadherin in TGF-Î²1-Induced Cell Cluster Formation of In Vitro Developing Human Langerhans-Type Dendritic Cells. The Journal of Immunology, 165(3), 1381â1386.

Authors 7

Elisabeth Riedl (first)
Johannes Stöckl (additional)
Otto Majdic (additional)
Clemens Scheinecker (additional)
Klemens Rappersberger (additional)
Walter Knapp (additional)
Herbert Strobl (additional)

References 46 Referenced 54

Dates

Type	When
Created	11 years, 4 months ago (April 22, 2014, 5:16 p.m.)
Deposited	8 months ago (Jan. 2, 2025, 1:01 p.m.)
Indexed	5 months ago (April 6, 2025, 5:03 p.m.)
Issued	25 years, 1 month ago (Aug. 1, 2000)
Published	25 years, 1 month ago (Aug. 1, 2000)
Published Print	25 years, 1 month ago (Aug. 1, 2000)

Funders 0

None

BibTeX

@article{Riedl_2000, title={Functional Involvement of E-Cadherin in TGF-β1-Induced Cell Cluster Formation of In Vitro Developing Human Langerhans-Type Dendritic Cells}, volume={165}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.165.3.1381}, DOI={10.4049/jimmunol.165.3.1381}, number={3}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Riedl, Elisabeth and Stöckl, Johannes and Majdic, Otto and Scheinecker, Clemens and Rappersberger, Klemens and Knapp, Walter and Strobl, Herbert}, year={2000}, month=aug, pages={1381–1386} }

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  "abstract": "<jats:title>Abstract</jats:title><jats:p>Epithelial Langerhans cells (LC) represent immature dendritic cells that require TGF-\u03b21 stimulation for their development. Little is known about the mechanisms regulating LC generation from their precursor cells. We demonstrate here that LC development from human CD34+ hemopoietic progenitor cells in response to TGF-\u03b21 costimulation (basic cytokine combination GM-CSF plus TNF-\u03b1, stem cell factor, and Flt3 ligand) is associated with pronounced cell cluster formation of developing LC precursor cells. This cell-clustering phenomenon requires hemopoietic progenitor cell differentiation, since it is first seen on day 4 after culture initiation of CD34+ cells. Cell cluster formation morphologically indicates progenitor cell development along the LC pathway, because parallel cultures set up in the absence of exogenous TGF-\u03b21 fail to form cell clusters and predominantly give rise to monocyte, but not LC, development (CD1a\u2212, lysozyme+, CD14+). TGF-\u03b21 costimulation of CD34+ cells induces neoexpression of the homophilic adhesion molecule E-cadherin in the absence of the E-cadherin heteroligand CD103. Addition of anti-E-cadherin mAb or mAbs to any of the constitutively expressed adhesion molecule (CD99, CD31, LFA-1, or CD18) to TGF-\u03b21-supplemented progenitor cell cultures inhibits LC precursor cell cluster formation, and this effect is, with the exception of anti-E-cadherin mAb, associated with inhibition of LC generation. Addition of anti-E-cadherin mAb to the culture allows cell cluster-independent generation of LC from CD34+ cells. Thus, functional E-cadherin expression and homotypic cell cluster formation represent a regular response of LC precursor cells to TGF-\u03b21 stimulation, and cytoadhesive interactions may modulate LC differentiation from hemopoietic progenitor cells.</jats:p>",
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