DOI: 10.4049/jimmunol.165.2.795. Phenotypic and Functional Characterization of Mouse Hepatic CD8α+ Lymphoid-Related Dendritic Cells

Phenotypic and Functional Characterization of Mouse Hepatic CD8α+ Lymphoid-Related Dendritic Cells

10.4049/jimmunol.165.2.795

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

AbstractRecently, attention has focussed on phenotypic and functional differences between classic myeloid dendritic cells (DC), and DC that reportedly develop from an early, committed lymphoid precursor. In mice, DC from these separate hemopoietic lineages differ by their surface expression of CD8α. We undertook a comparative study of CD8α+ (CD11blow; lymphoid-related) and CD8α− (CD11bhigh; myeloid) DC isolated from mouse liver. CD8α+ and CD8α− DC each constituted ≤1.0% of the freshly isolated, normal nonparenchymal cells (NPC). Both populations were enriched 10–15% by overnight culture and metrizamide density centrifugation. Flt3 ligand (Flt3L) potently induced equal expansion of both subsets in vivo. Tissue-resident CD8α+ DC, freshly isolated from Flt3L-treated mice, existed primarily as immature cells (CD11c+, CD11blow, CD40−/low, CD80low, CD86low, MHC class IIlow), consistent with previous observations regarding bulk DC freshly isolated from nonlymphoid tissues. Following overnight culture in GM-CSF, CD8α+ DC underwent phenotypic and functional maturation equivalent to that observed for CD8α− DC. CD95 ligand (FasL) mRNA was detected in both immature and mature DC of each subset. In vitro analysis confirmed that flow-sorted, mature CD8α+ and CD8α− DC were strong and equally efficient stimulators of allogeneic T cell proliferation in primary MLR. Both immunohistochemical and genomic DNA analysis revealed that in vivo, sorted CD8α+ DC trafficked from s.c. sites to T cell areas of allogeneic lymphoid tissue and were equally efficient at priming naive T cells compared with CD8α− DC. This is the first comparative study of lymphoid-related DC isolated from nonlymphoid tissue.

Bibliography

OâConnell, P. J., Morelli, A. E., Logar, A. J., & Thomson, A. W. (2000). Phenotypic and Functional Characterization of Mouse Hepatic CD8Î±+ Lymphoid-Related Dendritic Cells. The Journal of Immunology, 165(2), 795â803.

Authors 4

Peta J. O’Connell (first)
Adrian E. Morelli (additional)
Alison J. Logar (additional)
Angus W. Thomson (additional)

References 49 Referenced 85

Dates

Type	When
Created	11 years, 4 months ago (April 21, 2014, 11:02 p.m.)
Deposited	8 months ago (Jan. 2, 2025, 12:24 p.m.)
Indexed	4 months, 2 weeks ago (April 22, 2025, 3:16 a.m.)
Issued	25 years, 1 month ago (July 15, 2000)
Published	25 years, 1 month ago (July 15, 2000)
Published Print	25 years, 1 month ago (July 15, 2000)

Funders 0

None

BibTeX

@article{O_Connell_2000, title={Phenotypic and Functional Characterization of Mouse Hepatic CD8α+ Lymphoid-Related Dendritic Cells}, volume={165}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.165.2.795}, DOI={10.4049/jimmunol.165.2.795}, number={2}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={O’Connell, Peta J. and Morelli, Adrian E. and Logar, Alison J. and Thomson, Angus W.}, year={2000}, month=jul, pages={795–803} }

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  "abstract": "<jats:title>Abstract</jats:title><jats:p>Recently, attention has focussed on phenotypic and functional differences between classic myeloid dendritic cells (DC), and DC that reportedly develop from an early, committed lymphoid precursor. In mice, DC from these separate hemopoietic lineages differ by their surface expression of CD8\u03b1. We undertook a comparative study of CD8\u03b1+ (CD11blow; lymphoid-related) and CD8\u03b1\u2212 (CD11bhigh; myeloid) DC isolated from mouse liver. CD8\u03b1+ and CD8\u03b1\u2212 DC each constituted \u22641.0% of the freshly isolated, normal nonparenchymal cells (NPC). Both populations were enriched 10\u201315% by overnight culture and metrizamide density centrifugation. Flt3 ligand (Flt3L) potently induced equal expansion of both subsets in vivo. Tissue-resident CD8\u03b1+ DC, freshly isolated from Flt3L-treated mice, existed primarily as immature cells (CD11c+, CD11blow, CD40\u2212/low, CD80low, CD86low, MHC class IIlow), consistent with previous observations regarding bulk DC freshly isolated from nonlymphoid tissues. Following overnight culture in GM-CSF, CD8\u03b1+ DC underwent phenotypic and functional maturation equivalent to that observed for CD8\u03b1\u2212 DC. CD95 ligand (FasL) mRNA was detected in both immature and mature DC of each subset. In vitro analysis confirmed that flow-sorted, mature CD8\u03b1+ and CD8\u03b1\u2212 DC were strong and equally efficient stimulators of allogeneic T cell proliferation in primary MLR. Both immunohistochemical and genomic DNA analysis revealed that in vivo, sorted CD8\u03b1+ DC trafficked from s.c. sites to T cell areas of allogeneic lymphoid tissue and were equally efficient at priming naive T cells compared with CD8\u03b1\u2212 DC. This is the first comparative study of lymphoid-related DC isolated from nonlymphoid tissue.</jats:p>",
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