DOI: 10.4049/jimmunol.165.1.286. IL-10 Gene Expression Is Controlled by the Transcription Factors Sp1 and Sp3

IL-10 Gene Expression Is Controlled by the Transcription Factors Sp1 and Sp3

10.4049/jimmunol.165.1.286

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract IL-10 is an 18-kDa cytokine with a key role in homeostatic control of inflammatory and immune responses. We have investigated how transcription of the IL-10 gene is regulated, so as to be able to understand the circumstances of IL-10 expression in both health and disease. In the mouse, IL-10 gene expression is regulated by a TATA-type promoter with a critical cis-acting element containing GGA repeats located at −89 to −77. Its complementary sequence is similar to the cis-acting elements (TCC repeats) in the promoters of genes encoding epidermal growth factor receptor and CD58. All these elements comprise a common CCTCCT sequence with less conserved C + T-rich sequences. Eliminating this CCTCCT sequence results in a marked reduction in promoter activity, suggesting a necessary role in IL-10 gene expression. Despite its dissimilarity to the G + C-rich Sp1 consensus sequence (GC box), Sp1 and Sp3 transcription factors could be shown to bind to this motif. The requirement for Sp1 and Sp3 in transcription of IL-10 was confirmed using Drosophila SL2 cells, which lack endogenous Sp factors. These results suggest that the transcription of IL-10 is positively regulated by both Sp1 and Sp3.

Bibliography

Tone, M., Powell, M. J., Tone, Y., Thompson, S. A. J., & Waldmann, H. (2000). IL-10 Gene Expression Is Controlled by the Transcription Factors Sp1 and Sp3. The Journal of Immunology, 165(1), 286â291.

Authors 5

Masahide Tone (first)
Mark J Powell (additional)
Yukiko Tone (additional)
Sara A J Thompson (additional)
Herman Waldmann (additional)

References 41 Referenced 201

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Dates

Type	When
Created	11 years, 4 months ago (April 20, 2014, 8:07 p.m.)
Deposited	5 months ago (March 31, 2025, 4:01 a.m.)
Indexed	1 month ago (July 31, 2025, 11:51 p.m.)
Issued	25 years, 2 months ago (July 1, 2000)
Published	25 years, 2 months ago (July 1, 2000)
Published Online	25 years, 2 months ago (July 1, 2000)
Published Print	25 years, 2 months ago (July 1, 2000)

Funders 0

None

BibTeX

@article{Tone_2000, title={IL-10 Gene Expression Is Controlled by the Transcription Factors Sp1 and Sp3}, volume={165}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.165.1.286}, DOI={10.4049/jimmunol.165.1.286}, number={1}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Tone, Masahide and Powell, Mark J and Tone, Yukiko and Thompson, Sara A J and Waldmann, Herman}, year={2000}, month=jul, pages={286–291} }

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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>IL-10 is an 18-kDa cytokine with a key role in homeostatic control of inflammatory and immune responses. We have investigated how transcription of the IL-10 gene is regulated, so as to be able to understand the circumstances of IL-10 expression in both health and disease. In the mouse, IL-10 gene expression is regulated by a TATA-type promoter with a critical cis-acting element containing GGA repeats located at \u221289 to \u221277. Its complementary sequence is similar to the cis-acting elements (TCC repeats) in the promoters of genes encoding epidermal growth factor receptor and CD58. All these elements comprise a common CCTCCT sequence with less conserved C + T-rich sequences. Eliminating this CCTCCT sequence results in a marked reduction in promoter activity, suggesting a necessary role in IL-10 gene expression. Despite its dissimilarity to the G + C-rich Sp1 consensus sequence (GC box), Sp1 and Sp3 transcription factors could be shown to bind to this motif. The requirement for Sp1 and Sp3 in transcription of IL-10 was confirmed using Drosophila SL2 cells, which lack endogenous Sp factors. These results suggest that the transcription of IL-10 is positively regulated by both Sp1 and Sp3.</jats:p>",
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