DOI: 10.4049/jimmunol.164.6.2978. CD4 and CD8 Expression by Dendritic Cell Subtypes in Mouse Thymus and Spleen

CD4 and CD8 Expression by Dendritic Cell Subtypes in Mouse Thymus and Spleen

10.4049/jimmunol.164.6.2978

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

AbstractThe dendritic cells (DC) of mouse spleen and thymus were examined for expression of CD4 and CD8. Provided care was taken to avoid selective extraction or selective depletion of DC subpopulations, three main types of DC were detected in mouse spleen: a major new population of CD4+8− DEC-205low CD11bhigh DC, together with the previously described CD4−8− DEC-205low CD11bhigh DC and CD4−8αα+ DEC-205high CD11blow DC. The CD4 on the surface of the CD4+ splenic DC subpopulation was produced by the DC themselves, and CD4 RNA transcripts were present. Likewise, the CD8α on the surface of the splenic CD8+ DC was shown to be a product of the DC themselves, in agreement with earlier evidence. All three spleen DC types would be considered as mature, based on expression of CD80, CD86, and CD40 as well as on T cell stimulating function. Mouse thymuses appeared to contain two DC types; both were DEC-205highCD11blow, but they differed in the level of CD8αα expression. However, as well as this authenticated marker expression, immunofluorescent staining was also found to reflect a series of artifacts, due to the autofluorescence of contaminating cells and due to pickup of CD4 and CD8αβ. By constructing mice chimeric for the hemopoietic lineages using mixtures of wild-type bone marrow with CD4null or CD8αnull bone marrow, a marked pickup by thymic DC of Ags derived from thymocytes was demonstrated.

Bibliography

Vremec, D., Pooley, J., Hochrein, H., Wu, L., & Shortman, K. (2000). CD4 and CD8 Expression by Dendritic Cell Subtypes in Mouse Thymus and Spleen. The Journal of Immunology, 164(6), 2978â2986.

Authors 5

David Vremec (first)
Joanne Pooley (additional)
Hubertus Hochrein (additional)
Li Wu (additional)
Ken Shortman (additional)

References 32 Referenced 630

Dates

Type	When
Created	11 years, 4 months ago (April 22, 2014, 4:28 p.m.)
Deposited	7 months, 3 weeks ago (Jan. 2, 2025, 11:56 a.m.)
Indexed	22 hours, 23 minutes ago (Aug. 28, 2025, 8:27 a.m.)
Issued	25 years, 5 months ago (March 15, 2000)
Published	25 years, 5 months ago (March 15, 2000)
Published Print	25 years, 5 months ago (March 15, 2000)

Funders 0

None

BibTeX

@article{Vremec_2000, title={CD4 and CD8 Expression by Dendritic Cell Subtypes in Mouse Thymus and Spleen}, volume={164}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.164.6.2978}, DOI={10.4049/jimmunol.164.6.2978}, number={6}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Vremec, David and Pooley, Joanne and Hochrein, Hubertus and Wu, Li and Shortman, Ken}, year={2000}, month=mar, pages={2978–2986} }

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  "abstract": "<jats:title>Abstract</jats:title><jats:p>The dendritic cells (DC) of mouse spleen and thymus were examined for expression of CD4 and CD8. Provided care was taken to avoid selective extraction or selective depletion of DC subpopulations, three main types of DC were detected in mouse spleen: a major new population of CD4+8\u2212 DEC-205low CD11bhigh DC, together with the previously described CD4\u22128\u2212 DEC-205low CD11bhigh DC and CD4\u22128\u03b1\u03b1+ DEC-205high CD11blow DC. The CD4 on the surface of the CD4+ splenic DC subpopulation was produced by the DC themselves, and CD4 RNA transcripts were present. Likewise, the CD8\u03b1 on the surface of the splenic CD8+ DC was shown to be a product of the DC themselves, in agreement with earlier evidence. All three spleen DC types would be considered as mature, based on expression of CD80, CD86, and CD40 as well as on T cell stimulating function. Mouse thymuses appeared to contain two DC types; both were DEC-205highCD11blow, but they differed in the level of CD8\u03b1\u03b1 expression. However, as well as this authenticated marker expression, immunofluorescent staining was also found to reflect a series of artifacts, due to the autofluorescence of contaminating cells and due to pickup of CD4 and CD8\u03b1\u03b2. By constructing mice chimeric for the hemopoietic lineages using mixtures of wild-type bone marrow with CD4null or CD8\u03b1null bone marrow, a marked pickup by thymic DC of Ags derived from thymocytes was demonstrated.</jats:p>",
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