DOI: 10.4049/jimmunol.164.1.9. Cutting Edge: Signal-Regulatory Protein β1 Is a DAP12-Associated Activating Receptor Expressed in Myeloid Cells

Cutting Edge: Signal-Regulatory Protein β1 Is a DAP12-Associated Activating Receptor Expressed in Myeloid Cells

10.4049/jimmunol.164.1.9

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

AbstractSignal-regulatory proteins (SIRPs) are cell-surface glycoproteins expressed on myeloid and neural cells that have been shown to recruit SH2 domain-containing protein phosphatase 1 (SHP-1) and SHP-2 and to regulate receptor tyrosine kinase-coupled signaling. One SIRP of unknown function, designated SIRPβ1, contains a short cytoplasmic domain that lacks sequence motifs capable of recruiting SHP-1 and SHP-2. Using a SIRP-specific mAb, we show that SIRPβ1 is expressed in monocytes and dendritic cells and associates with the signal transduction molecule DAP12. SIRPβ1/DAP12 complex formation was required for efficient cell-surface expression of SIRPβ1. Stimulation of this complex induced tyrosine phosphorylation, mitogen-activated protein kinase activation, and cellular activation. Thus, SIRPβ1 is a new DAP12-associated receptor involved in the activation of myeloid cells.

Bibliography

Dietrich, J., Cella, M., Seiffert, M., BuÌhring, H.-J., & Colonna, M. (2000). Cutting Edge: Signal-Regulatory Protein Î²1 Is a DAP12-Associated Activating Receptor Expressed in Myeloid Cells. The Journal of Immunology, 164(1), 9â12.

Authors 5

Jes Dietrich (first)
Marina Cella (additional)
Martina Seiffert (additional)
Hans-Jörg Bühring (additional)
Marco Colonna (additional)

References 24 Referenced 133

Dates

Type	When
Created	11 years, 4 months ago (April 21, 2014, 6:07 p.m.)
Deposited	8 months ago (Jan. 2, 2025, 12:18 p.m.)
Indexed	1 month, 4 weeks ago (July 6, 2025, 1:45 p.m.)
Issued	25 years, 8 months ago (Jan. 1, 2000)
Published	25 years, 8 months ago (Jan. 1, 2000)
Published Print	25 years, 8 months ago (Jan. 1, 2000)

Funders 0

None

BibTeX

@article{Dietrich_2000, title={Cutting Edge: Signal-Regulatory Protein β1 Is a DAP12-Associated Activating Receptor Expressed in Myeloid Cells}, volume={164}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.164.1.9}, DOI={10.4049/jimmunol.164.1.9}, number={1}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Dietrich, Jes and Cella, Marina and Seiffert, Martina and Bühring, Hans-Jörg and Colonna, Marco}, year={2000}, month=jan, pages={9–12} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        6
      ]
    ],
    "date-time": "2025-07-06T17:45:13Z",
    "timestamp": 1751823913665,
    "version": "3.32.0"
  },
  "reference-count": 24,
  "publisher": "Oxford University Press (OUP)",
  "issue": "1",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2000,
        1,
        1
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>Signal-regulatory proteins (SIRPs) are cell-surface glycoproteins expressed on myeloid and neural cells that have been shown to recruit SH2 domain-containing protein phosphatase 1 (SHP-1) and SHP-2 and to regulate receptor tyrosine kinase-coupled signaling. One SIRP of unknown function, designated SIRP\u03b21, contains a short cytoplasmic domain that lacks sequence motifs capable of recruiting SHP-1 and SHP-2. Using a SIRP-specific mAb, we show that SIRP\u03b21 is expressed in monocytes and dendritic cells and associates with the signal transduction molecule DAP12. SIRP\u03b21/DAP12 complex formation was required for efficient cell-surface expression of SIRP\u03b21. Stimulation of this complex induced tyrosine phosphorylation, mitogen-activated protein kinase activation, and cellular activation. Thus, SIRP\u03b21 is a new DAP12-associated receptor involved in the activation of myeloid cells.</jats:p>",
  "DOI": "10.4049/jimmunol.164.1.9",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2014,
        4,
        21
      ]
    ],
    "date-time": "2014-04-21T22:07:57Z",
    "timestamp": 1398118077000
  },
  "page": "9-12",
  "source": "Crossref",
  "is-referenced-by-count": 133,
  "title": "Cutting Edge: Signal-Regulatory Protein \u03b21 Is a DAP12-Associated Activating Receptor Expressed in Myeloid Cells",
  "prefix": "10.1093",
  "volume": "164",
  "author": [
    {
      "given": "Jes",
      "family": "Dietrich",
      "sequence": "first",
      "affiliation": [
        {
          "name": "*Basel Institute for Immunology, Basel, Switzerland; and"
        }
      ]
    },
    {
      "given": "Marina",
      "family": "Cella",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "*Basel Institute for Immunology, Basel, Switzerland; and"
        }
      ]
    },
    {
      "given": "Martina",
      "family": "Seiffert",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "\u2020University of Tu\u0308bingen, Department of Internal Medicine II, Division of Hematology, Immunology and Oncology, Tu\u0308bingen, Germany"
        }
      ]
    },
    {
      "given": "Hans-Jo\u0308rg",
      "family": "Bu\u0308hring",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "\u2020University of Tu\u0308bingen, Department of Internal Medicine II, Division of Hematology, Immunology and Oncology, Tu\u0308bingen, Germany"
        }
      ]
    },
    {
      "given": "Marco",
      "family": "Colonna",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "*Basel Institute for Immunology, Basel, Switzerland; and"
        }
      ]
    }
  ],
  "member": "286",
  "reference": [
    {
      "key": "2022123020242611200_R1",
      "doi-asserted-by": "crossref",
      "unstructured": "Kharitonenkov, A., Z. Chen, I. Sures, H. Wang, J. Schilling, A. Ullrich. 1997. A family of proteins that inhibit signalling through tyrosine kinase receptors. Nature\u2008386: 181",
      "DOI": "10.1038/386181a0"
    },
    {
      "key": "2022123020242611200_R2",
      "doi-asserted-by": "crossref",
      "unstructured": "Timms, J. F., K. Carlberg, H. Gu, H. Chen, S. Kamatkar, M. J. Nadler, L. R. Rohrschneider, B. G. Neel. 1998. Identification of major binding proteins and substrates for the SH2-containing protein tyrosine phosphatase SHP-1 in macrophages. Mol. Cell. Biol.\u200818: 3838",
      "DOI": "10.1128/MCB.18.7.3838"
    },
    {
      "key": "2022123020242611200_R3",
      "doi-asserted-by": "crossref",
      "unstructured": "Veillette, A., E. Thibaudeau, S. Latour. 1998. High expression of inhibitory receptor SHPS-1 and its association with protein-tyrosine phosphatase SHP-1 in macrophages. J. Biol. Chem.\u2008273: 22719",
      "DOI": "10.1074/jbc.273.35.22719"
    },
    {
      "key": "2022123020242611200_R4",
      "doi-asserted-by": "crossref",
      "unstructured": "Adams, S., L. J. van der Laan, E. Vernon-Wilson, E. A. d. L. Renardel, C. D. Dopp, D. L. Dijkstra, D. L. Simmons, T. K. van den Berg. 1998. Signal-regulatory protein is selectively expressed by myeloid and neuronal cells. J. Immunol.\u2008161: 1853",
      "DOI": "10.4049/jimmunol.161.4.1853"
    },
    {
      "key": "2022123020242611200_R5",
      "doi-asserted-by": "crossref",
      "unstructured": "Sano, S., H. Ohnishi, A. Omori, J. Hasegawa, M. Kubota. 1997. BIT, an immune antigen receptor-like molecule in the brain. FEBS Lett.\u2008411: 327",
      "DOI": "10.1016/S0014-5793(97)00724-2"
    },
    {
      "key": "2022123020242611200_R6",
      "doi-asserted-by": "crossref",
      "unstructured": "Kim, S. O., J. Jiang, W. Yi, G. S. Feng, S. J. Frank. 1998. Involvement of the Src homology 2-containing tyrosine phosphatase SHP-2 in growth hormone signaling. J. Biol. Chem.\u2008273: 2344",
      "DOI": "10.1074/jbc.273.4.2344"
    },
    {
      "key": "2022123020242611200_R7",
      "doi-asserted-by": "crossref",
      "unstructured": "Saginario, C., H. Sterling, C. Beckers, R. Kobayashi, M. Solimena, E. Ullu, A. Vignery. 1998. MFR, a putative receptor mediating the fusion of macrophages. Mol. Cell. Biol.\u200818: 6213",
      "DOI": "10.1128/MCB.18.11.6213"
    },
    {
      "key": "2022123020242611200_R8",
      "doi-asserted-by": "crossref",
      "unstructured": "Oh, E. S., H. Gu, T. M. Saxton, J. F. Timms, S. Hausdorff, E. U. Frevert, B. B. Kahn, T. Pawson, B. G. Neel, S. M. Thomas. 1999. Regulation of early events in integrin signaling by protein tyrosine phosphatase SHP-2. Mol. Cell. Biol.\u200819: 3205",
      "DOI": "10.1128/MCB.19.4.3205"
    },
    {
      "key": "2022123020242611200_R9",
      "doi-asserted-by": "crossref",
      "unstructured": "Takada, T., T. Matozaki, H. Takeda, K. Fukunaga, T. Noguchi, Y. Fujioka, I. Okazaki, M. Tsuda, T. Yamao, F. Ochi, M. Kasuga. 1998. Roles of the complex formation of SHPS-1 with SHP-2 in insulin-stimulated mitogen-activated protein kinase activation. J. Biol. Chem.\u2008273: 9234",
      "DOI": "10.1074/jbc.273.15.9234"
    },
    {
      "key": "2022123020242611200_R10",
      "doi-asserted-by": "crossref",
      "unstructured": "Jiang, P., C. F. Lagenaur, V. Narayanan. 1999. Integrin-associated protein is a ligand for the P84 neural adhesion molecule. J. Biol. Chem.\u2008274: 559",
      "DOI": "10.1074/jbc.274.2.559"
    },
    {
      "key": "2022123020242611200_R11",
      "doi-asserted-by": "crossref",
      "unstructured": "Seiffert, M., C. Cant, Z. Chen, I. Rappold, W. Brugger, L. Kanz, E. J. Brown, A. Ullrich, and H. J. Buhring. 1999. Human Sirp is expressed on normal but not on subsets of leukemic myeloid cells and mediates cellular adhesion involving its counter-receptor CD47. Blood. In press.",
      "DOI": "10.1182/blood.V94.11.3633"
    },
    {
      "key": "2022123020242611200_R12",
      "doi-asserted-by": "crossref",
      "unstructured": "Timms, J. F., K. D. Swanson, A. Marie-Cardine, M. Raab, C. E. Rudd, B. Schraven, B. G. Neel. 1999. SHPS-1 is a scaffold for assembling distinct adhesion-regulated multi-protein complexes in macrophages. Curr. Biol.\u20089: 927",
      "DOI": "10.1016/S0960-9822(99)80401-1"
    },
    {
      "key": "2022123020242611200_R13",
      "doi-asserted-by": "crossref",
      "unstructured": "Tsuda, M., T. Matozaki, K. Fukunaga, Y. Fujioka, A. Imamoto, T. Noguchi, T. Takada, T. Yamao, H. Takeda, F. Ochi, T. Yamamoto, M. Kasuga. 1998. Integrin-mediated tyrosine phosphorylation of SHPS-1 and its association with SHP-2: roles of Fak and Src family kinases. J. Biol. Chem.\u2008273: 13223",
      "DOI": "10.1074/jbc.273.21.13223"
    },
    {
      "key": "2022123020242611200_R14",
      "doi-asserted-by": "crossref",
      "unstructured": "Lanier, L. L., B. C. Corliss, J. Wu, C. Leong, J. H. Phillips. 1998. Immunoreceptor DAP12 bearing a tyrosine-based activation motif is involved in activating NK cells. Nature\u2008391: 703",
      "DOI": "10.1038/35642"
    },
    {
      "key": "2022123020242611200_R15",
      "doi-asserted-by": "crossref",
      "unstructured": "Lanier, L. L., B. Corliss, J. Wu, J. H. Phillips. 1998. Association of DAP12 with activating CD94/NKG2C NK cell receptors. Immunity\u20088: 693",
      "DOI": "10.1016/S1074-7613(00)80574-9"
    },
    {
      "key": "2022123020242611200_R16",
      "doi-asserted-by": "crossref",
      "unstructured": "Smith, K. M., J. Wu, A. B. Bakker, J. H. Phillips, L. L. Lanier. 1998. Ly-49D and Ly-49H associate with mouse DAP12 and form activating receptors. J. Immunol.\u2008161: 7",
      "DOI": "10.4049/jimmunol.161.1.7"
    },
    {
      "key": "2022123020242611200_R17",
      "doi-asserted-by": "crossref",
      "unstructured": "Colonna, M., F. Navarro, T. Bellon, M. Llano, P. Garcia, J. Samaridis, L. Angman, M. Cella, M. Lopez-Botet. 1997. A common inhibitory receptor for major histocompatibility complex class I molecules on human lymphoid and myelomonocytic cells. J. Exp. Med.\u2008186: 1809",
      "DOI": "10.1084/jem.186.11.1809"
    },
    {
      "key": "2022123020242611200_R18",
      "doi-asserted-by": "crossref",
      "unstructured": "Campbell, K. S., M. Cella, M. Carretero, M. Lopez-Botet, M. Colonna. 1998. Signaling through human killer cell activating receptors triggers tyrosine phosphorylation of an associated protein complex. Eur. J. Immunol.\u200828: 599",
      "DOI": "10.1002/(SICI)1521-4141(199802)28:02<599::AID-IMMU599>3.0.CO;2-F"
    },
    {
      "key": "2022123020242611200_R19",
      "doi-asserted-by": "crossref",
      "unstructured": "Wu, J., Y. Song, A. B. Bakker, S. Bauer, T. Spies, L. L. Lanier, J. H. Phillips. 1999. An activating immunoreceptor complex formed by NKG2D and DAP10. Science\u2008285: 730",
      "DOI": "10.1126/science.285.5428.730"
    },
    {
      "key": "2022123020242611200_R20",
      "doi-asserted-by": "crossref",
      "unstructured": "Chang, C., J. Dietrich, A. G. Harpur, J. A. Lindquist, A. Haude, Y. W. Loke, A. King, M. Colonna, J. Trowsdale, M. Wilson. 1999. KAP10: a novel transmembrane adapter protein genetically linked to DAP12 but with unique signalling properties. J. Immunol.\u2008163: 4651",
      "DOI": "10.4049/jimmunol.163.9.4651"
    },
    {
      "key": "2022123020242611200_R21",
      "doi-asserted-by": "crossref",
      "unstructured": "Tomasello, E., L. Olcese, F. Vely, C. Geourgeon, M. Blery, A. Moqrich, D. Gautheret, M. Djabali, M. G. Mattei, E. Vivier. 1998. Gene structure, expression pattern, and biological activity of mouse killer cell activating receptor-associated protein (KARAP)/DAP-12. J. Biol. Chem.\u2008273: 34115",
      "DOI": "10.1074/jbc.273.51.34115"
    },
    {
      "key": "2022123020242611200_R22",
      "doi-asserted-by": "crossref",
      "unstructured": "Gosselin, P., L. H. Mason, J. Willette-Brown, J. R. Ortaldo, D. W. McVicar, S. K. Anderson. 1999. Induction of DAP12 phosphorylation, calcium mobilization, and cytokine secretion by Ly49H. J. Leukocyte Biol.\u200866: 165",
      "DOI": "10.1002/jlb.66.1.165"
    },
    {
      "key": "2022123020242611200_R23",
      "doi-asserted-by": "crossref",
      "unstructured": "McVicar, D. W., L. S. Taylor, P. Gosselin, J. Willette-Brown, A. I. Mikhael, R. L. Geahlen, M. C. Nakamura, P. Linnemeyer, W. E. Seaman, S. K. Anderson, et al\u20081998. DAP12-mediated signal transduction in natural killer cells: a dominant role for the Syk protein-tyrosine kinase. J. Biol. Chem.\u2008273: 32934",
      "DOI": "10.1074/jbc.273.49.32934"
    },
    {
      "key": "2022123020242611200_R24",
      "doi-asserted-by": "crossref",
      "unstructured": "Bakker, A. B., E. Baker, G. R. Sutherland, J. H. Phillips, L. L. Lanier. 1999. Myeloid DAP12-associating lectin (MDL)-1 is a cell surface receptor involved in the activation of myeloid cells. Proc. Natl. Acad. Sci. USA\u200896: 9792",
      "DOI": "10.1073/pnas.96.17.9792"
    }
  ],
  "container-title": "The Journal of Immunology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.aai.org/jimmunol/article-pdf/164/1/9/1111458/9.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://journals.aai.org/jimmunol/article-pdf/164/1/9/1111458/9.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2025,
        1,
        2
      ]
    ],
    "date-time": "2025-01-02T17:18:17Z",
    "timestamp": 1735838297000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.aai.org/jimmunol/article/164/1/9/6697/Cutting-Edge-Signal-Regulatory-Protein-1-Is-a"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2000,
        1,
        1
      ]
    ]
  },
  "references-count": 24,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          2000,
          1,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.4049/jimmunol.164.1.9",
  "relation": {},
  "ISSN": [
    "0022-1767",
    "1550-6606"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        2000,
        1,
        1
      ]
    ]
  }
}