DOI: 10.4049/jimmunol.163.1.62. Expression of a Variant of CD28 on a Subpopulation of Human NK Cells: Implications for B7-Mediated Stimulation of NK Cells

Expression of a Variant of CD28 on a Subpopulation of Human NK Cells: Implications for B7-Mediated Stimulation of NK Cells

10.4049/jimmunol.163.1.62

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract The ability of NK cells to kill tumor cells is controlled by a balance between activating and inhibitory signals transduced by distinct receptors. In murine tumor models, the costimulatory molecule B7.1 not only acts as a positive trigger for NK-mediated cytotoxicity but can also overcome negative signaling transduced by MHC class I molecules. In this study, we have evaluated the potential of human B7.1-CD28 interaction as an activating trigger for human blood NK cells. Using multiparameter flow cytometric analysis and a panel of different CD28 mAbs, we show that human peripheral blood NK cells (defined by CD56+, CD16+, and CD3− surface expression) express the CD28 costimulatory receptor, with its detection totally dependent on the mAb used. In addition, the level of CD28 varies among individuals and on different NK cell lines, irrespective of CD28 steady-state mRNA levels. By performing Ab binding studies on T cells, our data strongly suggest that binding of two of the anti-CD28 Abs (clones 9.3 and CD28.2) is to a different epitope to that recognized by clones L293 and YTH913.12, which is perhaps modified in the CD28 molecule expressed by the NK cells. We also show that B7.1 enhances the NK-mediated lysis of NK-sensitive but not of NK-resistant tumor cells and that this increased lysis is dependent on CD28-B7 interactions as shown by the ability of Abs to block this lysis. Coculture of the B7.1-positive NK-sensitive cells also led to the activation of the NK cells, as determined by the expression of CD69, CD25, and HLA class II.

Bibliography

Galea-Lauri, J., Darling, D., Gan, S.-U., Krivochtchapov, L., Kuiper, M., GaÌken, J., Souberbielle, B., & Farzaneh, F. (1999). Expression of a Variant of CD28 on a Subpopulation of Human NK Cells: Implications for B7-Mediated Stimulation of NK Cells. The Journal of Immunology, 163(1), 62â70.

Authors 8

Joanna Galea-Lauri (first)
David Darling (additional)
Shu-Uin Gan (additional)
Leonid Krivochtchapov (additional)
Marcel Kuiper (additional)
Joop Gäken (additional)
Bernard Souberbielle (additional)
Farzin Farzaneh (additional)

References 31 Referenced 70

Dates

Type	When
Created	2 years, 8 months ago (Jan. 1, 2023, 12:28 p.m.)
Deposited	8 months ago (Jan. 2, 2025, 1:16 p.m.)
Indexed	4 months, 3 weeks ago (April 10, 2025, 9:48 a.m.)
Issued	26 years, 2 months ago (July 1, 1999)
Published	26 years, 2 months ago (July 1, 1999)
Published Print	26 years, 2 months ago (July 1, 1999)

Funders 0

None

BibTeX

@article{Galea_Lauri_1999, title={Expression of a Variant of CD28 on a Subpopulation of Human NK Cells: Implications for B7-Mediated Stimulation of NK Cells}, volume={163}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.163.1.62}, DOI={10.4049/jimmunol.163.1.62}, number={1}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Galea-Lauri, Joanna and Darling, David and Gan, Shu-Uin and Krivochtchapov, Leonid and Kuiper, Marcel and Gäken, Joop and Souberbielle, Bernard and Farzaneh, Farzin}, year={1999}, month=jul, pages={62–70} }

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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>The ability of NK cells to kill tumor cells is controlled by a balance between activating and inhibitory signals transduced by distinct receptors. In murine tumor models, the costimulatory molecule B7.1 not only acts as a positive trigger for NK-mediated cytotoxicity but can also overcome negative signaling transduced by MHC class I molecules. In this study, we have evaluated the potential of human B7.1-CD28 interaction as an activating trigger for human blood NK cells. Using multiparameter flow cytometric analysis and a panel of different CD28 mAbs, we show that human peripheral blood NK cells (defined by CD56+, CD16+, and CD3\u2212 surface expression) express the CD28 costimulatory receptor, with its detection totally dependent on the mAb used. In addition, the level of CD28 varies among individuals and on different NK cell lines, irrespective of CD28 steady-state mRNA levels. By performing Ab binding studies on T cells, our data strongly suggest that binding of two of the anti-CD28 Abs (clones 9.3 and CD28.2) is to a different epitope to that recognized by clones L293 and YTH913.12, which is perhaps modified in the CD28 molecule expressed by the NK cells. We also show that B7.1 enhances the NK-mediated lysis of NK-sensitive but not of NK-resistant tumor cells and that this increased lysis is dependent on CD28-B7 interactions as shown by the ability of Abs to block this lysis. Coculture of the B7.1-positive NK-sensitive cells also led to the activation of the NK cells, as determined by the expression of CD69, CD25, and HLA class II.</jats:p>",
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