DOI: 10.4049/jimmunol.163.1.11. Cutting Edge: Proteasome Involvement in the Degradation of Unassembled Ig Light Chains

Cutting Edge: Proteasome Involvement in the Degradation of Unassembled Ig Light Chains

10.4049/jimmunol.163.1.11

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Several studies on disposal of nonsecreted Ig L chains have identified the endoplasmic reticulum as the site of degradation. Here, we examine degradation of a nonsecreted Ig L chain, T15L, and an experimentally endoplasmic reticulum-retained secretion-competent L chain, D16L, in the absence of H chains. We demonstrate that 1) degradation is specifically impaired by the proteasome-specific inhibitors carboxybenzyl-leucyl-leucyl-leucine vinyl sulfone (Z-L3VS) and lactacystin, 2) L chain degradation occurs early in the biosynthetic pathway, and 3) degradation does not require vesicular transport. Our findings indicate that previous assertions of L chain disposal within the endoplasmic reticulum must be modified. To our knowledge, we provide the first direct evidence supporting a new paradigm for removal of nonsecreted Ig L chains via dislocation to cytosolic proteasomes.

Bibliography

OâHare, T., Wiens, G. D., Whitcomb, E. A., Enns, C. A., & Rittenberg, M. B. (1999). Cutting Edge: Proteasome Involvement in the Degradation of Unassembled Ig Light Chains. The Journal of Immunology, 163(1), 11â14.

Authors 5

Thomas O’Hare (first)
Gregory D. Wiens (additional)
Elizabeth A. Whitcomb (additional)
Caroline A. Enns (additional)
Marvin B. Rittenberg (additional)

References 27 Referenced 18

Dates

Type	When
Created	2 years, 7 months ago (Jan. 1, 2023, 12:31 p.m.)
Deposited	7 months, 3 weeks ago (Jan. 2, 2025, 1:17 p.m.)
Indexed	7 months, 3 weeks ago (Jan. 3, 2025, 12:33 a.m.)
Issued	26 years, 1 month ago (July 1, 1999)
Published	26 years, 1 month ago (July 1, 1999)
Published Print	26 years, 1 month ago (July 1, 1999)

Funders 0

None

BibTeX

@article{O_Hare_1999, title={Cutting Edge: Proteasome Involvement in the Degradation of Unassembled Ig Light Chains}, volume={163}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.163.1.11}, DOI={10.4049/jimmunol.163.1.11}, number={1}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={O’Hare, Thomas and Wiens, Gregory D. and Whitcomb, Elizabeth A. and Enns, Caroline A. and Rittenberg, Marvin B.}, year={1999}, month=jul, pages={11–14} }

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