DOI: 10.4049/jimmunol.162.6.3601. Soluble Fas Ligand Is Chemotactic for Human Neutrophilic Polymorphonuclear Leukocytes

Soluble Fas Ligand Is Chemotactic for Human Neutrophilic Polymorphonuclear Leukocytes

10.4049/jimmunol.162.6.3601

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract It has been recently shown that Fas ligand (FasL) expression on islet β grafts results in neutrophilic infiltration and graft rejection. In this study, we show that human recombinant soluble FasL is endowed with potent chemotactic properties toward human neutrophilic polymorphonuclear leukocytes (neutrophils) at concentrations incapable of inducing cell apoptosis. Furthermore, neutrophils exposed to soluble FasL did not display detectable change of intracellular Ca2+ and did not undergo superoxide production or exocytosis of primary and secondary granules. Our results show that FasL is a potent chemoattractant for human neutrophils without evoking their secretory responses. This finding suggests a novel proinflammatory function for this ligand and may help to clarify the mechanism governing FasL-mediated graft rejection, thereby offering rational bases for controlling and modulating FasL-based immunotherapies.

Bibliography

Ottonello, L., Tortolina, G., Amelotti, M., & Dallegri, F. (1999). Soluble Fas Ligand Is Chemotactic for Human Neutrophilic Polymorphonuclear Leukocytes. The Journal of Immunology, 162(6), 3601â3606.

Authors 4

Luciano Ottonello (first)
Giuseppe Tortolina (additional)
Massimo Amelotti (additional)
Franco Dallegri (additional)

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Dates

Type	When
Created	2 years, 8 months ago (Dec. 31, 2022, 10:14 p.m.)
Deposited	8 months ago (Jan. 2, 2025, 1:15 p.m.)
Indexed	1 month, 2 weeks ago (July 14, 2025, 11:24 p.m.)
Issued	26 years, 5 months ago (March 15, 1999)
Published	26 years, 5 months ago (March 15, 1999)
Published Print	26 years, 5 months ago (March 15, 1999)

Funders 0

None

BibTeX

@article{Ottonello_1999, title={Soluble Fas Ligand Is Chemotactic for Human Neutrophilic Polymorphonuclear Leukocytes}, volume={162}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.162.6.3601}, DOI={10.4049/jimmunol.162.6.3601}, number={6}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Ottonello, Luciano and Tortolina, Giuseppe and Amelotti, Massimo and Dallegri, Franco}, year={1999}, month=mar, pages={3601–3606} }

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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>It has been recently shown that Fas ligand (FasL) expression on islet \u03b2 grafts results in neutrophilic infiltration and graft rejection. In this study, we show that human recombinant soluble FasL is endowed with potent chemotactic properties toward human neutrophilic polymorphonuclear leukocytes (neutrophils) at concentrations incapable of inducing cell apoptosis. Furthermore, neutrophils exposed to soluble FasL did not display detectable change of intracellular Ca2+ and did not undergo superoxide production or exocytosis of primary and secondary granules. Our results show that FasL is a potent chemoattractant for human neutrophils without evoking their secretory responses. This finding suggests a novel proinflammatory function for this ligand and may help to clarify the mechanism governing FasL-mediated graft rejection, thereby offering rational bases for controlling and modulating FasL-based immunotherapies.</jats:p>",
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