DOI: 10.4049/jimmunol.161.7.3501. The Molecular and Functional Characterization of a Dominant Minor H Antigen, H60

The Molecular and Functional Characterization of a Dominant Minor H Antigen, H60

10.4049/jimmunol.161.7.3501

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

AbstractMinor histocompatibility (H) Ags elicit T cell responses and thereby cause chronic graft rejection and graft-vs-host disease among MHC identical individuals. Although numerous independent H loci exist in mice of a given MHC haplotype, certain H Ags dominate the immune response and are thus of considerable conceptual and therapeutic importance. To identify these H Ags and their genes, lacZ-inducible CD8+ T cell hybrids were generated by immunizing C57BL/6 (B6) mice with MHC identical BALB.B spleen cells. The cDNA clones encoding the precursor for the antigenic peptide/Kb MHC class I complex were isolated by expression cloning using the BCZ39.84 T cell as a probe. The cDNAs defined a new H locus (termed H60), located on mouse chromosome 10, and encoded a novel protein that contains the naturally processed octapeptide LTFNYRNL (LYL8) presented by the Kb MHC molecule. Southern blot analysis revealed that the H60 locus was polymorphic among the BALB and the B6 strains. However, none of the H60 transcripts expressed in the donor BALB spleen were detected in the host B6 strain. The expression and immunogenicity of the LYL8/Kb complex in BALB.B and CXB recombinant inbred strains strongly suggested that the H60 locus may account for one of the previously described antigenic activity among these strains. The results establish the source of an immunodominant autosomal minor H Ag that, by its differential transcription in the donor vs the host strains, provides a novel peptide/MHC target for host CD8+ T cells.

Bibliography

Malarkannan, S., Shih, P. P., Eden, P. A., Horng, T., Zuberi, A. R., Christianson, G., Roopenian, D., & Shastri, N. (1998). The Molecular and Functional Characterization of a Dominant Minor H Antigen, H60. The Journal of Immunology, 161(7), 3501â3509.

Authors 8

Subramaniam Malarkannan (first)
Patty P. Shih (additional)
Peter A. Eden (additional)
Tiffany Horng (additional)
Aamir R. Zuberi (additional)
Greg Christianson (additional)
Derry Roopenian (additional)
Nilabh Shastri (additional)

References 53 Referenced 197

Dates

Type	When
Created	2 years, 8 months ago (Dec. 30, 2022, 4:14 p.m.)
Deposited	8 months ago (Jan. 2, 2025, 1:51 p.m.)
Indexed	1 week, 4 days ago (Aug. 23, 2025, 1:25 a.m.)
Issued	26 years, 11 months ago (Oct. 1, 1998)
Published	26 years, 11 months ago (Oct. 1, 1998)
Published Print	26 years, 11 months ago (Oct. 1, 1998)

Funders 0

None

BibTeX

@article{Malarkannan_1998, title={The Molecular and Functional Characterization of a Dominant Minor H Antigen, H60}, volume={161}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.161.7.3501}, DOI={10.4049/jimmunol.161.7.3501}, number={7}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Malarkannan, Subramaniam and Shih, Patty P. and Eden, Peter A. and Horng, Tiffany and Zuberi, Aamir R. and Christianson, Greg and Roopenian, Derry and Shastri, Nilabh}, year={1998}, month=oct, pages={3501–3509} }

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    {
      "key": "2022123021115934100_R1",
      "doi-asserted-by": "crossref",
      "unstructured": "Goulmy, E., R. Schipper, J. Pool, E. Blokland, J. H. F. Falkenburg, J. Vossen, A. Gratwohl, G. B. Vogelsang, H. C. Van Houwelingen, J. J. Van Rood. 1996. Mismatches of minor histocompatibility antigens between HLA-identical donors and recipients and the development of graft-versus-host disease after bone marrow transplantation. N. Engl. J. Med.\u2008334: 281",
      "DOI": "10.1056/NEJM199602013340501"
    },
    {
      "key": "2022123021115934100_R2",
      "unstructured": "Little, C. C.. 1941. The genetics of tumor transplantation. G. D. Snell, ed. Biology of the Laboratory Mouse\u2008279-309. Blakiston, Philadelphia."
    },
    {
      "key": "2022123021115934100_R3",
      "doi-asserted-by": "crossref",
      "unstructured": "Snell, G. D.. 1948. Methods for the study of histocompatibility genes. J. Genet.\u200849: 87",
      "DOI": "10.1007/BF02986826"
    },
    {
      "key": "2022123021115934100_R4",
      "doi-asserted-by": "crossref",
      "unstructured": "Doolittle, D. P., M. T. Davisson, J. N. Guidi, M. C. Green. 1996. Catalog of mutant genes and polymorphic loci. M. F. Lyon, and S. Rastan, and S. D. M. Brown, eds. Genetic Variants and Strains of the Laboratory Mouse\u200817-854. Oxford University Press, Oxford, U.K.",
      "DOI": "10.1093/oso/9780198548690.003.0002"
    },
    {
      "key": "2022123021115934100_R5",
      "doi-asserted-by": "crossref",
      "unstructured": "Wallny, H.-J., H.-G. Rammensee. 1990. Identification of classical minor histocompatibility antigen as cell-derived peptide. Nature\u2008343: 275",
      "DOI": "10.1038/343275a0"
    },
    {
      "key": "2022123021115934100_R6",
      "doi-asserted-by": "crossref",
      "unstructured": "Loveland, B., C.-R. Wang, H. Yonekawa, E. Hermel, K. F. Lindahl. 1990. Maternally transmitted histocompatibility antigen of mice: a hydrophobic peptide of a mitochondrially encoded protein. Cell\u200860: 971",
      "DOI": "10.1016/0092-8674(90)90345-F"
    },
    {
      "key": "2022123021115934100_R7",
      "doi-asserted-by": "crossref",
      "unstructured": "Bhuyan, P. K., L. L. Young, K. Fischer Lindhal, G. W. Butcher. 1997. Identification of the rat maternally transmitted minor histocompatibility antigen. J. Immunol.\u2008158: 3753",
      "DOI": "10.4049/jimmunol.158.8.3753"
    },
    {
      "key": "2022123021115934100_R8",
      "doi-asserted-by": "crossref",
      "unstructured": "Morse, M.-C., G. Bleau, V. M. Dabhi, F. Hetu, E. A. Drobetsky, K. F. Lindahl, C. Perreault. 1996. The COI mitochondrial gene encodes a minor histocompatibility antigen presented by H2\u2013M3. J. Immunol.\u2008156: 3301",
      "DOI": "10.4049/jimmunol.156.9.3301"
    },
    {
      "key": "2022123021115934100_R9",
      "doi-asserted-by": "crossref",
      "unstructured": "Scott, D. M., I. E. Ehrmann, P. S. Ellis, C. E. Bishop, A. I. Agulnik, E. Simpson, M. J. Mitchell. 1995. Identification of a mouse male-specific transplantation antigen, H-Y. Nature\u2008376: 695",
      "DOI": "10.1038/376695a0"
    },
    {
      "key": "2022123021115934100_R10",
      "doi-asserted-by": "crossref",
      "unstructured": "Wang, W., L. R. Meadows, J. M. M. den Haan, N. E. Sherman, Y. Chen, E. Blokland, J. Shabanowitz, A. I. Agulnik, R. C. Hendrickson, C. E. Bishop, D. F. Hunt, E. Goulmy, V. H. Engelhard. 1995. Human H-Y: a male-specific histocompatibility antigen derived from the SMCY protein. Science\u2008269: 1588",
      "DOI": "10.1126/science.7667640"
    },
    {
      "key": "2022123021115934100_R11",
      "doi-asserted-by": "crossref",
      "unstructured": "Greenfield, A., D. Scott, D. Pennisi, I. Ehrmann, P. Ellis, L. Cooper, E. Simpson, P. Koopman. 1996. An H-YDb epitope is encoded by a novel mouse Y chromosome gene. Nat. Genet.\u200814: 474",
      "DOI": "10.1038/ng1296-474"
    },
    {
      "key": "2022123021115934100_R12",
      "doi-asserted-by": "crossref",
      "unstructured": "den Haan, J. M. M., R. E. Bontrop, J. Pool, N. Sherman, E. Blokland, V. H. Engelhard, D. F. Hunt, E. Goulmy. 1996. Conservation of minor histocompatibility antigens between human and non-human primates. Eur. J. Immunol.\u200826: 2680",
      "DOI": "10.1002/eji.1830261120"
    },
    {
      "key": "2022123021115934100_R13",
      "doi-asserted-by": "crossref",
      "unstructured": "Nevala, W. K., P. J. Wettstein. 1996. The preferential cytolytic T lymphocyte response to immunodominant minor histocompatibility antigen peptides. Transplantation\u200862: 283",
      "DOI": "10.1097/00007890-199607270-00022"
    },
    {
      "key": "2022123021115934100_R14",
      "doi-asserted-by": "crossref",
      "unstructured": "Wolpert, E., L. Franksson, K. Karre. 1995. Dominant and cryptic antigens in the MHC class I restricted T cell response across a complex minor histocompatibility barrier: analysis and mapping by elution of cellular peptides. Int. Immunol.\u20087: 919",
      "DOI": "10.1093/intimm/7.6.919"
    },
    {
      "key": "2022123021115934100_R15",
      "doi-asserted-by": "crossref",
      "unstructured": "Wettstein, P. J., D. W. Bailey. 1982. Immunodominance in the immune response to \u201cmultiple\u201d histocompatibility antigens. Immunogenetics\u200816: 47",
      "DOI": "10.1007/BF00364441"
    },
    {
      "key": "2022123021115934100_R16",
      "doi-asserted-by": "crossref",
      "unstructured": "Wettstein, P. J.. 1986. Immunodominance in the T-cell response to multiple non-H-2 histocompatibility antigens. Immunogenetics\u200824: 24",
      "DOI": "10.1007/BF00372294"
    },
    {
      "key": "2022123021115934100_R17",
      "doi-asserted-by": "crossref",
      "unstructured": "Wettstein, P. J.. 1986. Immunodominance in the T cell response to multiple non-H-2 histocompatibility antigens. III. Single histocompatibility antigens dominate the male antigen. J. Immunol.\u2008137: 2073",
      "DOI": "10.1007/BF00372294"
    },
    {
      "key": "2022123021115934100_R18",
      "doi-asserted-by": "crossref",
      "unstructured": "Bevan, M. J.. 1975. Interaction antigens detected by cytotoxic T cells with the major histocompatibility complex as modifier. Nature\u2008256: 419",
      "DOI": "10.1038/256419a0"
    },
    {
      "key": "2022123021115934100_R19",
      "doi-asserted-by": "crossref",
      "unstructured": "von Boehmer, H., P. Kisielow. 1990. Self-nonself discrimination by T cells. Science\u2008248: 1369",
      "DOI": "10.1126/science.1972594"
    },
    {
      "key": "2022123021115934100_R20",
      "doi-asserted-by": "crossref",
      "unstructured": "Goulmy, E.. 1996. Human minor histocompatibility antigens. Curr. Opin. Immunol.\u20088: 75",
      "DOI": "10.1016/S0952-7915(96)80108-7"
    },
    {
      "key": "2022123021115934100_R21",
      "doi-asserted-by": "crossref",
      "unstructured": "Auchincloss, H., H. Sultan. 1996. Antigen processing and presentation in transplantation. Curr. Opin. Immunol.\u20088: 681",
      "DOI": "10.1016/S0952-7915(96)80086-0"
    },
    {
      "key": "2022123021115934100_R22",
      "doi-asserted-by": "crossref",
      "unstructured": "Karttunen, J., S. Sanderson, N. Shastri. 1992. Detection of rare antigen presenting cells by the lacZ T-cell activation assay suggests an expression cloning strategy for T-cell antigens. Proc. Natl. Acad. Sci. USA\u200889: 6020",
      "DOI": "10.1073/pnas.89.13.6020"
    },
    {
      "key": "2022123021115934100_R23",
      "doi-asserted-by": "crossref",
      "unstructured": "Malarkannan, S., M. Afkarian, N. Shastri. 1995. A rare cryptic translation product is presented by Kb MHC class I molecule to alloreactive T-cells. J. Exp. Med.\u2008182: 1739",
      "DOI": "10.1084/jem.182.6.1739"
    },
    {
      "key": "2022123021115934100_R24",
      "doi-asserted-by": "crossref",
      "unstructured": "Shastri, N.. 1996. Needles in haystacks: identifying specific peptide antigens for T-cells. Curr. Opin. Immunol.\u20088: 271",
      "DOI": "10.1016/S0952-7915(96)80067-7"
    },
    {
      "key": "2022123021115934100_R25",
      "doi-asserted-by": "crossref",
      "unstructured": "Mendoza, L., P. Paz, A. R. Zuberi, G. Christianson, D. C. Roopenian, N. Shastri. 1997. Minors held by majors: the H13 minor histocompatibility locus defined as a peptide/MHC class I complex. Immunity\u20087: 461",
      "DOI": "10.1016/S1074-7613(00)80368-4"
    },
    {
      "key": "2022123021115934100_R26",
      "doi-asserted-by": "crossref",
      "unstructured": "Cerottini, J. C., H. D. Engers, H. R. Macdonald, T. Brunner. 1974. Generation of cytotoxic T lymphocytes in vitro. I. Response of normal and immune mouse spleen cells in mixed leukocyte cultures. J. Exp. Med.\u2008140: 703",
      "DOI": "10.1084/jem.140.3.703"
    },
    {
      "key": "2022123021115934100_R27",
      "doi-asserted-by": "crossref",
      "unstructured": "Shastri, N., B. Malissen, L. Hood. 1985. Ia-transfected L-cell fibroblasts present a lysozyme peptide but not the native protein to lysozyme-specific T cells. Proc. Natl. Acad. Sci. USA\u200882: 5885",
      "DOI": "10.1073/pnas.82.17.5885"
    },
    {
      "key": "2022123021115934100_R28",
      "doi-asserted-by": "crossref",
      "unstructured": "Roopenian, D. C., M. B. Widmer, C. G. Orosz, F. H. Bach. 1983. Response against single minor histocompatibility antigens. I. Functional and immunogenetic analysis of cloned cytolytic T cells. J. Immunol.\u2008131: 2135",
      "DOI": "10.4049/jimmunol.131.5.2135"
    },
    {
      "key": "2022123021115934100_R29",
      "doi-asserted-by": "crossref",
      "unstructured": "Roopenian, D. C., A. P. Davis. 1989. Responses against antigens encoded by the H-3 histocompatibility locus: antigens stimulating class I MHC- and class II MHC-restricted T cells are encoded by separate genes. Immunogenetics\u200830: 335",
      "DOI": "10.1007/BF02425273"
    },
    {
      "key": "2022123021115934100_R30",
      "doi-asserted-by": "crossref",
      "unstructured": "Sanderson, S., N. Shastri. 1994. LacZ inducible peptide/MHC specific T-hybrids. Int. Immunol.\u20086: 369",
      "DOI": "10.1093/intimm/6.3.369"
    },
    {
      "key": "2022123021115934100_R31",
      "doi-asserted-by": "crossref",
      "unstructured": "Karttunen, J., N. Shastri. 1991. Measurement of ligand induced activation in single viable T-cells using the lacZ reporter gene. Proc. Natl. Acad. Sci. USA\u200888: 3972",
      "DOI": "10.1073/pnas.88.9.3972"
    },
    {
      "key": "2022123021115934100_R32",
      "doi-asserted-by": "crossref",
      "unstructured": "Van Bleek, G. M., S. G. Nathenson. 1990. Isolation of an endogenously processed immunodominant viral peptide from the class I H-2Kb molecule. Nature\u2008348: 213",
      "DOI": "10.1038/348213a0"
    },
    {
      "key": "2022123021115934100_R33",
      "doi-asserted-by": "crossref",
      "unstructured": "Malarkannan, S., F. Gonzalez, V. Nguyen, G. Adair, N. Shastri. 1996. Alloreactive CD8+ T-cells can recognize unusual, rare and unique processed peptide/MHC complexes. J. Immunol.\u2008157: 4464",
      "DOI": "10.4049/jimmunol.157.10.4464"
    },
    {
      "key": "2022123021115934100_R34",
      "unstructured": "Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, K. Struhl. 1994. Current Protocols in Molecular Biology John Wiley & Sons, Inc, New York."
    },
    {
      "key": "2022123021115934100_R35",
      "doi-asserted-by": "crossref",
      "unstructured": "Shastri, N., F. Gonzalez. 1993. Endogenous generation and presentation of the OVA peptide/Kb complex to T-cells. J. Immunol.\u2008150: 2724",
      "DOI": "10.4049/jimmunol.150.7.2724"
    },
    {
      "key": "2022123021115934100_R36",
      "doi-asserted-by": "crossref",
      "unstructured": "Malarkannan, S., T. Serwold, V. Nguyen, L. A. Sherman, N. Shastri. 1996. The mouse mammary tumor virus env gene is the source of a CD8+ T-cell-stimulating peptide presented by a major histocompatibility complex class I molecule in a murine thymoma. Proc. Natl. Acad. Sci. USA\u200893: 13991",
      "DOI": "10.1073/pnas.93.24.13991"
    },
    {
      "key": "2022123021115934100_R37",
      "doi-asserted-by": "crossref",
      "unstructured": "Dietrich, W., H. Katz, S. E. Lincoln, H. S. Shin, J. Friedman, N. C. Dracopoli, E. S. Lander. 1992. A genetic map of the mouse suitable for typing intraspecific crosses. Genetics\u2008131: 423",
      "DOI": "10.1093/genetics/131.2.423"
    },
    {
      "key": "2022123021115934100_R38",
      "doi-asserted-by": "crossref",
      "unstructured": "Rowe, L. B., J. H. Nadeau, R. Turner, W. N. Frankel, V. A. Letts, J. T. Eppig, M. S. H. Ko, S. J. Thurston, E. H. Birkenmeier. 1994. Maps from two interspecific backcross DNA panels available as a community genetic mapping resource. Mamm. Genome\u20085: 253",
      "DOI": "10.1007/BF00389540"
    },
    {
      "key": "2022123021115934100_R39",
      "doi-asserted-by": "crossref",
      "unstructured": "Porgador, A., J. W. Yewdell, Y. Deng, J. R. Bennink, R. N. Germain. 1997. Localization, quantitation, and in situ detection of specific peptide-MHC class I complexes using a monoclonal antibody. Immunity\u20086: 715",
      "DOI": "10.1016/S1074-7613(00)80447-1"
    },
    {
      "key": "2022123021115934100_R40",
      "doi-asserted-by": "crossref",
      "unstructured": "Rotzschke, O., K. Falk, H.-J. Wallny, S. Faath, H.-G. Rammensee. 1990. Characterization of naturally occurring minor histocompatibility peptides including H-4 and H-Y. Science\u2008249: 283",
      "DOI": "10.1126/science.1695760"
    },
    {
      "key": "2022123021115934100_R41",
      "doi-asserted-by": "crossref",
      "unstructured": "Malarkannan, S., S. Goth, D. R. Buchholz, N. Shastri. 1995. The role of MHC class I molecules in the generation of endogenous peptide/MHC complexes. J. Immunol.\u2008154: 585",
      "DOI": "10.4049/jimmunol.154.2.585"
    },
    {
      "key": "2022123021115934100_R42",
      "doi-asserted-by": "crossref",
      "unstructured": "Falk, K., O. Rotzschke, S. Stevanovic, G. Jung, H.-G. Rammensee. 1991. Allele-specific motifs revealed by sequencing of self-peptides eluted from MHC molecules. Nature\u2008351: 290",
      "DOI": "10.1038/351290a0"
    },
    {
      "key": "2022123021115934100_R43",
      "doi-asserted-by": "crossref",
      "unstructured": "Bluestone, J. A., S. Jameson, S. Miller, R. I. Dick. 1992. Peptide-induced conformational changes in class I heavy chains alter major histocompatibility complex recognition. J. Exp. Med.\u2008176: 1757",
      "DOI": "10.1084/jem.176.6.1757"
    },
    {
      "key": "2022123021115934100_R44",
      "doi-asserted-by": "crossref",
      "unstructured": "Sherman, L. A., S. Chattopadhyay, J. A. Biggs, R. F. Dick, J. A. Bluestone. 1993. Alloantibodies can discriminate class I major histocompatibility complex molecules associated with various endogenous peptides. Proc. Natl. Acad. Sci. USA\u200890: 6949",
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      "DOI": "10.1172/JCI118832"
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      "DOI": "10.1128/jvi.71.7.5244-5250.1997"
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      "DOI": "10.1126/science.1546328"
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