DOI: 10.4049/jimmunol.161.2.585. TCR Transgenic Mice in Which Usage of Transgenic α- and β-Chains Is Highly Dependent on the Level of Selecting Ligand

TCR Transgenic Mice in Which Usage of Transgenic α- and β-Chains Is Highly Dependent on the Level of Selecting Ligand

10.4049/jimmunol.161.2.585

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

AbstractWe have produced a TCR transgenic mouse that uses a TCR derived from a Th1 clone that is specific for residues 64 to 76 of the d allele of murine hemoglobin presented by I-Ek. Examination of these TCR transgenic mice on an H-2k/k background that expressed the nonstimulatory s allele of murine hemoglobin revealed that these mice express many endogenous TCR chains from both α and β loci. We found that this transgenic TCR is also very inefficient at mediating β selection, thereby showing a direct linkage between β selection and allelic exclusion of TCR β. We have also examined these mice on MHC backgrounds that have reduced levels of I-Ek and found that positive selection of cells with high levels of the transgenic TCR depends greatly on the ligand density. Decreasing the selecting ligand density is a means of reducing the number of available selecting niches, and the data reveal that the 3.L2 TCR is used sparingly for positive selection under conditions where the number of niches becomes limiting. The results, therefore, show a way that T cells may get to the periphery with two self-restricted TCRs: one that efficiently mediates positive selection, and another that is inefficient at positive selection with the available niches.

Bibliography

Kersh, G. J., Donermeyer, D. L., Frederick, K. E., White, J. M., Hsu, B. L., & Allen, P. M. (1998). TCR Transgenic Mice in Which Usage of Transgenic Î±- and Î²-Chains Is Highly Dependent on the Level of Selecting Ligand. The Journal of Immunology, 161(2), 585â593.

Authors 6

Gilbert J. Kersh (first)
David L. Donermeyer (additional)
Katherine E. Frederick (additional)
J. Michael White (additional)
Benjamin L. Hsu (additional)
Paul M. Allen (additional)

References 36 Referenced 47

Dates

Type	When
Created	2 years, 8 months ago (Dec. 31, 2022, 9:20 a.m.)
Deposited	8 months ago (Jan. 2, 2025, noon)
Indexed	1 month ago (Aug. 3, 2025, 12:09 a.m.)
Issued	27 years, 1 month ago (July 15, 1998)
Published	27 years, 1 month ago (July 15, 1998)
Published Print	27 years, 1 month ago (July 15, 1998)

Funders 0

None

BibTeX

@article{Kersh_1998, title={TCR Transgenic Mice in Which Usage of Transgenic α- and β-Chains Is Highly Dependent on the Level of Selecting Ligand}, volume={161}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.161.2.585}, DOI={10.4049/jimmunol.161.2.585}, number={2}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Kersh, Gilbert J. and Donermeyer, David L. and Frederick, Katherine E. and White, J. Michael and Hsu, Benjamin L. and Allen, Paul M.}, year={1998}, month=jul, pages={585–593} }

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