DOI: 10.4049/jimmunol.161.12.7031. Molecular and Immunologic Characterization of a Highly Cross-Reactive Two EF-Hand Calcium-Binding Alder Pollen Allergen, Aln g 4: Structural Basis for Calcium-Modulated IgE Recognition

Molecular and Immunologic Characterization of a Highly Cross-Reactive Two EF-Hand Calcium-Binding Alder Pollen Allergen, Aln g 4: Structural Basis for Calcium-Modulated IgE Recognition

10.4049/jimmunol.161.12.7031

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

AbstractSerum IgE was used to isolate a cDNA coding for a 9.4-kDa two EF-hand calcium-binding allergen, Aln g 4, from a λgt11 expression cDNA library constructed from alder (Alnus glutinosa) pollen. rAln g 4 was overexpressed in Escherichia coliand purified to homogeneity. It reacted with serum IgE from 18% of pollen-allergic patients (n = 122); shared IgE epitopes with homologous allergens present in tree, grass, and weed pollens; and thus belongs to a family of highly cross-reactive pollen allergens. Exposure of two E. coli-expressed rAln g 4 fragments comprising amino acids 1–41 and 42–85 to patients’ IgE Abs, as well as to a rabbit antiserum raised against purified rAln g 4, indicated that most of the B cell epitopes reside in the N-terminal portion of the protein. IgE recognition of Aln g 4 was strongly modulated by the presence or absence of calcium. Circular dichroism analysis of rAln g 4 revealed that the protein consisted mostly of α helical secondary structure and possessed a remarkable thermal stability and refolding capacity, a property that was greatly reduced after calcium depletion. Circular dichroism analysis of the calcium-bound and apo form of rAln g 4 indicated that calcium-induced modulation of IgE binding could be due to changes in the protein conformation. Purified rAln g 4 elicited dose-dependent basophil histamine release and immediate type skin reactions in sensitized patients. It may hence be useful for allergy diagnosis and for specific immunotherapy.

Bibliography

Hayek, B., Vangelista, L., Pastore, A., Sperr, W. R., Valent, P., Vrtala, S., Niederberger, V., Twardosz, A., Kraft, D., & Valenta, R. (1998). Molecular and Immunologic Characterization of a Highly Cross-Reactive Two EF-Hand Calcium-Binding Alder Pollen Allergen, Aln g 4: Structural Basis for Calcium-Modulated IgE Recognition. The Journal of Immunology, 161(12), 7031â7039.

Authors 10

Brigitte Hayek (first)
Luca Vangelista (additional)
Annalisa Pastore (additional)
Wolfgang R. Sperr (additional)
Peter Valent (additional)
Susanne Vrtala (additional)
Verena Niederberger (additional)
Anna Twardosz (additional)
Dietrich Kraft (additional)
Rudolf Valenta (additional)

References 56 Referenced 58

Dates

Type	When
Created	2 years, 8 months ago (Dec. 30, 2022, 12:40 p.m.)
Deposited	7 months, 4 weeks ago (Jan. 2, 2025, 1:09 p.m.)
Indexed	3 months, 2 weeks ago (May 17, 2025, 2:47 a.m.)
Issued	26 years, 8 months ago (Dec. 15, 1998)
Published	26 years, 8 months ago (Dec. 15, 1998)
Published Print	26 years, 8 months ago (Dec. 15, 1998)

Funders 0

None

BibTeX

@article{Hayek_1998, title={Molecular and Immunologic Characterization of a Highly Cross-Reactive Two EF-Hand Calcium-Binding Alder Pollen Allergen, Aln g 4: Structural Basis for Calcium-Modulated IgE Recognition}, volume={161}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.161.12.7031}, DOI={10.4049/jimmunol.161.12.7031}, number={12}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Hayek, Brigitte and Vangelista, Luca and Pastore, Annalisa and Sperr, Wolfgang R. and Valent, Peter and Vrtala, Susanne and Niederberger, Verena and Twardosz, Anna and Kraft, Dietrich and Valenta, Rudolf}, year={1998}, month=dec, pages={7031–7039} }

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  "abstract": "<jats:title>Abstract</jats:title><jats:p>Serum IgE was used to isolate a cDNA coding for a 9.4-kDa two EF-hand calcium-binding allergen, Aln g 4, from a \u03bbgt11 expression cDNA library constructed from alder (Alnus glutinosa) pollen. rAln g 4 was overexpressed in Escherichia coliand purified to homogeneity. It reacted with serum IgE from 18% of pollen-allergic patients (n = 122); shared IgE epitopes with homologous allergens present in tree, grass, and weed pollens; and thus belongs to a family of highly cross-reactive pollen allergens. Exposure of two E. coli-expressed rAln g 4 fragments comprising amino acids 1\u201341 and 42\u201385 to patients\u2019 IgE Abs, as well as to a rabbit antiserum raised against purified rAln g 4, indicated that most of the B cell epitopes reside in the N-terminal portion of the protein. IgE recognition of Aln g 4 was strongly modulated by the presence or absence of calcium. Circular dichroism analysis of rAln g 4 revealed that the protein consisted mostly of \u03b1 helical secondary structure and possessed a remarkable thermal stability and refolding capacity, a property that was greatly reduced after calcium depletion. Circular dichroism analysis of the calcium-bound and apo form of rAln g 4 indicated that calcium-induced modulation of IgE binding could be due to changes in the protein conformation. Purified rAln g 4 elicited dose-dependent basophil histamine release and immediate type skin reactions in sensitized patients. It may hence be useful for allergy diagnosis and for specific immunotherapy.</jats:p>",
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