DOI: 10.4049/jimmunol.160.5.2388. Contribution of Cells at the Site of DNA Vaccination to the Generation of Antigen-Specific Immunity and Memory

Contribution of Cells at the Site of DNA Vaccination to the Generation of Antigen-Specific Immunity and Memory

10.4049/jimmunol.160.5.2388

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Gene gun-mediated DNA vaccination stimulates an immune response characterized by the activation of IgG-secreting B cells and IFN-γ-secreting T cells. To monitor the contribution of cells at the site of vaccination to this process, transfected skin was periodically removed and grafted onto naive recipients. Immediate removal of vaccinated skin abrogated the development of an immune response. Low-level IgG production was stimulated when the vaccination site was left in place for ≥5 h, with the strength of this response increasing the longer the site remained intact (for up to 2 wk). Measurable primary T cell responses were observed in animals whose vaccination site remained in place for ≥1 day. Skin grafts transferred 0 to 24 h postvaccination stimulated a primary immune response in naive recipients. Memory B and T cells were generated in animals whose site of vaccination remained intact for 5 to 12 h. Skin transferred within 12 h of vaccination triggered memory B and T cell development in graft recipients, while the removal of skin &gt;12 h postvaccination did not reduce memory in vaccinated mice. These findings suggest that 1) primary immunity is induced by cells that migrate rapidly from the site of immunization, 2) nonmigratory cells influence the magnitude of this primary response, and 3) migratory cells alone are responsible for the induction of immunologic memory.

Bibliography

Klinman, D. M., Sechler, J. M. G., Conover, J., Gu, M., & Rosenberg, A. S. (1998). Contribution of Cells at the Site of DNA Vaccination to the Generation of Antigen-Specific Immunity and Memory. The Journal of Immunology, 160(5), 2388â2392.

Authors 5

Dennis M. Klinman (first)
Joan M. G. Sechler (additional)
Jacqueline Conover (additional)
Mili Gu (additional)
Amy S. Rosenberg (additional)

References 34 Referenced 87

Dates

Type	When
Created	2 years, 7 months ago (Dec. 31, 2022, 8:18 p.m.)
Deposited	7 months, 3 weeks ago (Jan. 2, 2025, 12:37 p.m.)
Indexed	4 months, 3 weeks ago (March 31, 2025, 9:04 a.m.)
Issued	27 years, 5 months ago (March 1, 1998)
Published	27 years, 5 months ago (March 1, 1998)
Published Print	27 years, 5 months ago (March 1, 1998)

Funders 0

None

BibTeX

@article{Klinman_1998, title={Contribution of Cells at the Site of DNA Vaccination to the Generation of Antigen-Specific Immunity and Memory}, volume={160}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.160.5.2388}, DOI={10.4049/jimmunol.160.5.2388}, number={5}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Klinman, Dennis M. and Sechler, Joan M. G. and Conover, Jacqueline and Gu, Mili and Rosenberg, Amy S.}, year={1998}, month=mar, pages={2388–2392} }

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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>Gene gun-mediated DNA vaccination stimulates an immune response characterized by the activation of IgG-secreting B cells and IFN-\u03b3-secreting T cells. To monitor the contribution of cells at the site of vaccination to this process, transfected skin was periodically removed and grafted onto naive recipients. Immediate removal of vaccinated skin abrogated the development of an immune response. Low-level IgG production was stimulated when the vaccination site was left in place for \u22655 h, with the strength of this response increasing the longer the site remained intact (for up to 2 wk). Measurable primary T cell responses were observed in animals whose vaccination site remained in place for \u22651 day. Skin grafts transferred 0 to 24 h postvaccination stimulated a primary immune response in naive recipients. Memory B and T cells were generated in animals whose site of vaccination remained intact for 5 to 12 h. Skin transferred within 12 h of vaccination triggered memory B and T cell development in graft recipients, while the removal of skin &amp;gt;12 h postvaccination did not reduce memory in vaccinated mice. These findings suggest that 1) primary immunity is induced by cells that migrate rapidly from the site of immunization, 2) nonmigratory cells influence the magnitude of this primary response, and 3) migratory cells alone are responsible for the induction of immunologic memory.</jats:p>",
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