DOI: 10.4049/jimmunol.160.4.1666. The Cytokine Profile Expressed by Human Dendritic Cells Is Dependent on Cell Subtype and Mode of Activation

The Cytokine Profile Expressed by Human Dendritic Cells Is Dependent on Cell Subtype and Mode of Activation
10.4049/jimmunol.160.4.1666
Crossref journal-article
Oxford University Press (OUP)
The Journal of Immunology (286)
Abstract
AbstractIn the present study, we have analyzed the pattern of cytokines expressed by two independent dendritic cell (DC) subpopulations generated in vitro from human cord blood CD34+ progenitors cultured with granulocyte-macrophage CSF and TNF-α. Molecularly, we confirmed the phenotypic differences discriminating the two subsets: E-cadherin mRNA was only detected in CD1a+-derived DC, whereas CD68 and factor XIIIa mRNAs were observed exclusively in CD14+-derived DC. Semiquantitative reverse-transcriptase PCR analysis revealed that both DC subpopulations spontaneously expressed IL-1α, IL-1β, IL-6, IL-7, IL-12 (p35 and p40), IL-15, IL-18, TNF-α, TGF-β, macrophage CSF, and granulocyte-macrophage CSF, but not IL-2, IL-3, IL-4, IL-5, IL-9, and IFN-γ transcripts. Both subpopulations were shown to secrete IL-12 after CD40 triggering. Interestingly, only the CD14+-derived DC secreted IL-10 after CD40 activation, strengthening the notion that the two DC subpopulations indeed represent two independent pathways of DC development. Furthermore, both DC subpopulations expressed IL-13 mRNA and protein following activation with PMA-ionomycin, but not with CD40 ligand, in contrast to IL-12 and IL-10, revealing the existence of different pathways for DC activation. Finally, we confirmed the expression of IL-7, IL-10, and IL-13 mRNA by CD4+CD11c+CD3− DC isolated ex vivo from tonsillar germinal centers. Thus, CD14+-derived DC expressing IL-10 and factor XIIIa seemed more closely related to germinal center dendritic cellsGCDC than to Langerhans cells.
Bibliography
de Saint-Vis, B., Fugier-Vivier, I., Massacrier, C., Gaillard, C., Vanbervliet, B., AiÌt-Yahia, S., Banchereau, J., Liu, Y.-J., Lebecque, S., & Caux, C. (1998). The Cytokine Profile Expressed by Human Dendritic Cells Is Dependent on Cell Subtype and Mode of Activation. The Journal of Immunology, 160(4), 1666â1676.
Authors 10
Blandine de Saint-Vis (first)
Isabelle Fugier-Vivier (additional)
Catherine Massacrier (additional)
Claude Gaillard (additional)
Béatrice Vanbervliet (additional)
Smina Aït-Yahia (additional)
Jacques Banchereau (additional)
Yong-Jun Liu (additional)
Serge Lebecque (additional)
Christophe Caux (additional)
References 74 Referenced 329
Dates
Type	When
Created	2 years, 8 months ago (Dec. 30, 2022, 1:14 p.m.)
Deposited	8 months ago (Jan. 2, 2025, 12:09 p.m.)
Indexed	1 month ago (Aug. 2, 2025, 12:30 a.m.)
Issued	27 years, 6 months ago (Feb. 15, 1998)
Published	27 years, 6 months ago (Feb. 15, 1998)
Published Print	27 years, 6 months ago (Feb. 15, 1998)
Funders 0
None
BibTeX
@article{de_Saint_Vis_1998, title={The Cytokine Profile Expressed by Human Dendritic Cells Is Dependent on Cell Subtype and Mode of Activation}, volume={160}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.160.4.1666}, DOI={10.4049/jimmunol.160.4.1666}, number={4}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={de Saint-Vis, Blandine and Fugier-Vivier, Isabelle and Massacrier, Catherine and Gaillard, Claude and Vanbervliet, Béatrice and Aït-Yahia, Smina and Banchereau, Jacques and Liu, Yong-Jun and Lebecque, Serge and Caux, Christophe}, year={1998}, month=feb, pages={1666–1676} }
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  "abstract": "<jats:title>Abstract</jats:title><jats:p>In the present study, we have analyzed the pattern of cytokines expressed by two independent dendritic cell (DC) subpopulations generated in vitro from human cord blood CD34+ progenitors cultured with granulocyte-macrophage CSF and TNF-\u03b1. Molecularly, we confirmed the phenotypic differences discriminating the two subsets: E-cadherin mRNA was only detected in CD1a+-derived DC, whereas CD68 and factor XIIIa mRNAs were observed exclusively in CD14+-derived DC. Semiquantitative reverse-transcriptase PCR analysis revealed that both DC subpopulations spontaneously expressed IL-1\u03b1, IL-1\u03b2, IL-6, IL-7, IL-12 (p35 and p40), IL-15, IL-18, TNF-\u03b1, TGF-\u03b2, macrophage CSF, and granulocyte-macrophage CSF, but not IL-2, IL-3, IL-4, IL-5, IL-9, and IFN-\u03b3 transcripts. Both subpopulations were shown to secrete IL-12 after CD40 triggering. Interestingly, only the CD14+-derived DC secreted IL-10 after CD40 activation, strengthening the notion that the two DC subpopulations indeed represent two independent pathways of DC development. Furthermore, both DC subpopulations expressed IL-13 mRNA and protein following activation with PMA-ionomycin, but not with CD40 ligand, in contrast to IL-12 and IL-10, revealing the existence of different pathways for DC activation. Finally, we confirmed the expression of IL-7, IL-10, and IL-13 mRNA by CD4+CD11c+CD3\u2212 DC isolated ex vivo from tonsillar germinal centers. Thus, CD14+-derived DC expressing IL-10 and factor XIIIa seemed more closely related to germinal center dendritic cellsGCDC than to Langerhans cells.</jats:p>",
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