DOI: 10.4049/jimmunol.160.1.7. Cutting Edge: p21-Activated Kinase (PAK) Is Required for Fas-Induced JNK Activation in Jurkat Cells

Cutting Edge: p21-Activated Kinase (PAK) Is Required for Fas-Induced JNK Activation in Jurkat Cells

10.4049/jimmunol.160.1.7

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract The process of apoptosis is a critical component of normal immune system development and homeostasis, and in many cells this involves signaling through the c-Jun amino terminal kinase (JNK) pathway. In Jurkat T cells, Fas-induced JNK activity is dependent upon activation of the caspase cascades known to be central components of the apoptotic program. We show in Jurkat cell lines expressing a dominant negative PAK construct that PAK signaling is necessary for JNK activation in response to Fas receptor cross-linking. Inhibition of JNK activation induced by Fas does not impair cell death as assessed by DNA fragmentation. However, expression of the catalytically active C terminus of PAK2, which is generated through caspase action during Fas-mediated apoptosis, induces Jurkat cell apoptosis. We conclude that PAK activity resulting from caspase-mediated cleavage is a necessary component of JNK activation induced by Fas receptor signaling and that PAK2 can contribute to the induction of cell death.

Bibliography

Rudel, T., Zenke, F. T., Chuang, T.-H., & Bokoch, G. M. (1998). Cutting Edge: p21-Activated Kinase (PAK) Is Required for Fas-Induced JNK Activation in Jurkat Cells. The Journal of Immunology, 160(1), 7â11.

Authors 4

Thomas Rudel (first)
Frank T Zenke (additional)
Tsung-Hsien Chuang (additional)
Gary M Bokoch (additional)

References 47 Referenced 79

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Dates

Type	When
Created	2 years, 8 months ago (Jan. 1, 2023, 7:29 p.m.)
Deposited	5 months ago (March 30, 2025, 10:09 p.m.)
Indexed	2 months, 3 weeks ago (June 10, 2025, 3:05 p.m.)
Issued	27 years, 8 months ago (Jan. 1, 1998)
Published	27 years, 8 months ago (Jan. 1, 1998)
Published Online	27 years, 8 months ago (Jan. 1, 1998)
Published Print	27 years, 8 months ago (Jan. 1, 1998)

Funders 0

None

BibTeX

@article{Rudel_1998, title={Cutting Edge: p21-Activated Kinase (PAK) Is Required for Fas-Induced JNK Activation in Jurkat Cells}, volume={160}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.160.1.7}, DOI={10.4049/jimmunol.160.1.7}, number={1}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Rudel, Thomas and Zenke, Frank T and Chuang, Tsung-Hsien and Bokoch, Gary M}, year={1998}, month=jan, pages={7–11} }

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