DOI: 10.4049/jimmunol.155.7.3433. IL-1 beta converting enzyme (ICE) is not required for apoptosis induced by lymphokine deprivation in an IL-2-dependent T cell line.

IL-1 beta converting enzyme (ICE) is not required for apoptosis induced by lymphokine deprivation in an IL-2-dependent T cell line.

10.4049/jimmunol.155.7.3433

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Clonal T cells undergo programmed cell death (PCD) or apoptosis when cultured without the appropriate cytokines. The cysteine protease, IL-1 beta converting enzyme (ICE), is implicated in apoptosis based on its structural similarity to the PCD gene, ced-3, in Caenorhabditis elegans and the induction of PCD in fibroblasts transfected with recombinant ICE. We show that the murine IL-2-dependent CTLL T cell line expresses ICE but not IL-1 beta. Interestingly, ICE mRNA and protein levels increase during apoptosis. Yet inhibition of ICE enzymatic activity (&gt; 90%) with either of two cell-permeable ICE inhibitors does not abrogate or delay apoptosis following IL-2 deprivation, as measured by DNA fragmentation and viability. Our results suggest that ICE is not required for apoptosis in lymphokine-deprived T cells.

Bibliography

Vasilakos, J. P., Ghayur, T., Carroll, R. T., Giegel, D. A., Saunders, J. M., Quintal, L., Keane, K. M., & Shivers, B. D. (1995). IL-1 beta converting enzyme (ICE) is not required for apoptosis induced by lymphokine deprivation in an IL-2-dependent T cell line. The Journal of Immunology, 155(7), 3433â3442.

Authors 8

J P Vasilakos (first)
T Ghayur (additional)
R T Carroll (additional)
D A Giegel (additional)
J M Saunders (additional)
L Quintal (additional)
K M Keane (additional)
B D Shivers (additional)

References 0 Referenced 27

None

Dates

Type	When
Created	2 years, 7 months ago (Dec. 31, 2022, 7:25 a.m.)
Deposited	7 months, 3 weeks ago (Jan. 2, 2025, 12:54 p.m.)
Indexed	3 months, 1 week ago (May 18, 2025, 12:04 p.m.)
Issued	29 years, 10 months ago (Oct. 1, 1995)
Published	29 years, 10 months ago (Oct. 1, 1995)
Published Print	29 years, 10 months ago (Oct. 1, 1995)

Funders 0

None

BibTeX

@article{Vasilakos_1995, title={IL-1 beta converting enzyme (ICE) is not required for apoptosis induced by lymphokine deprivation in an IL-2-dependent T cell line.}, volume={155}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.155.7.3433}, DOI={10.4049/jimmunol.155.7.3433}, number={7}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Vasilakos, J P and Ghayur, T and Carroll, R T and Giegel, D A and Saunders, J M and Quintal, L and Keane, K M and Shivers, B D}, year={1995}, month=oct, pages={3433–3442} }

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