DOI: 10.4049/jimmunol.155.3.1032. Antigen-dependent clonal expansion of a trace population of antigen-specific CD4+ T cells in vivo is dependent on CD28 costimulation and inhibited by CTLA-4.

Antigen-dependent clonal expansion of a trace population of antigen-specific CD4+ T cells in vivo is dependent on CD28 costimulation and inhibited by CTLA-4.

10.4049/jimmunol.155.3.1032

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract The importance of CD28 costimulation to a primary T cell response in vivo was assessed in an adoptive transfer system where a small population of peptide-specific CD4+ TCR transgenic T cells can be physically tracked. Ag-dependent clonal expansion of the transgenic T cells in draining lymph nodes was blocked by cyclosporin A and required a CD28 signal that was completely inhibited by CTLA-4-Ig or a combination of anti-B7-1 and anti-B7-2 mAbs, but not by either Ab alone. In vivo treatment with the combination of anti-B7-1 and anti-B7-2 mAbs also blocked conversion of the Ag-specific T cells to the activated phenotype. In contrast, anti-CTLA-4 Fab greatly enhanced the in vivo clonal expansion of the Ag-specific T cells. These results suggest that Ag-driven proliferation and phenotype conversion of naive CD4+ T cells is dependent on CD28-derived signals and is inhibited by CTLA-4.

Bibliography

Kearney, E. R., Walunas, T. L., Karr, R. W., Morton, P. A., Loh, D. Y., Bluestone, J. A., & Jenkins, M. K. (1995). Antigen-dependent clonal expansion of a trace population of antigen-specific CD4+ T cells in vivo is dependent on CD28 costimulation and inhibited by CTLA-4. The Journal of Immunology, 155(3), 1032â1036.

Authors 7

E R Kearney (first)
T L Walunas (additional)
R W Karr (additional)
P A Morton (additional)
D Y Loh (additional)
J A Bluestone (additional)
M K Jenkins (additional)

References 0 Referenced 253

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 31, 2022, 6:48 a.m.)
Deposited	7 months, 4 weeks ago (Jan. 2, 2025, 11:55 a.m.)
Indexed	2 months, 4 weeks ago (June 2, 2025, 11:50 a.m.)
Issued	30 years, 1 month ago (Aug. 1, 1995)
Published	30 years, 1 month ago (Aug. 1, 1995)
Published Print	30 years, 1 month ago (Aug. 1, 1995)

Funders 0

None

BibTeX

@article{Kearney_1995, title={Antigen-dependent clonal expansion of a trace population of antigen-specific CD4+ T cells in vivo is dependent on CD28 costimulation and inhibited by CTLA-4.}, volume={155}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.155.3.1032}, DOI={10.4049/jimmunol.155.3.1032}, number={3}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Kearney, E R and Walunas, T L and Karr, R W and Morton, P A and Loh, D Y and Bluestone, J A and Jenkins, M K}, year={1995}, month=aug, pages={1032–1036} }

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