DOI: 10.4049/jimmunol.155.11.5268. Mouse VH7183 recombination signal sequences mediate recombination more frequently than those of VHJ558

Mouse VH7183 recombination signal sequences mediate recombination more frequently than those of VHJ558

10.4049/jimmunol.155.11.5268

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Mouse VH gene segments are conventionally classified into 13 families on the basis of sequence similarity. The 7183 family lies close to the 3’ end of the locus and is preferentially used in BALB/c mice; J558, the largest family, lies close to the 5’ end of the VH stretch and is preferentially used in C57BL/6 mice. To investigate whether differential effectiveness of the RSSs in the two families might contribute to the overusage of 7183 in the primary repertoire of BALB/c, we constructed recombination substrates in which the recombination signal sequences (RSSs) of VH segments 7183 and J558 compete with each other for a single RSS, DFL16.1, after transfection into two transformed cell lines derived from C57BL/6 and two cell lines from BALB/c mice. In both strains, the 7183 RSS was found to be preferentially used (83%). Thus, the 7183 RSS mediates recombination more frequently than does that of J558, and this preference must thereby influence the primary repertoire, but the strain difference cannot be accounted for by a difference in the RSSs.

Bibliography

Connor, A. M., Fanning, L. J., Celler, J. W., Hicks, L. K., Ramsden, D. A., & Wu, G. E. (1995). Mouse VH7183 recombination signal sequences mediate recombination more frequently than those of VHJ558. The Journal of Immunology, 155(11), 5268â5272.

Authors 6

A M Connor (first)
L J Fanning (additional)
J W Celler (additional)
L K Hicks (additional)
D A Ramsden (additional)
G E Wu (additional)

References 0 Referenced 36

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 31, 2022, 7:18 a.m.)
Deposited	5 months, 1 week ago (March 29, 2025, 8:47 p.m.)
Indexed	5 months, 1 week ago (March 30, 2025, 11:07 a.m.)
Issued	29 years, 9 months ago (Dec. 1, 1995)
Published	29 years, 9 months ago (Dec. 1, 1995)
Published Online	29 years, 9 months ago (Dec. 1, 1995)
Published Print	29 years, 9 months ago (Dec. 1, 1995)

Funders 0

None

BibTeX

@article{Connor_1995, title={Mouse VH7183 recombination signal sequences mediate recombination more frequently than those of VHJ558}, volume={155}, ISSN={0022-1767}, url={http://dx.doi.org/10.4049/jimmunol.155.11.5268}, DOI={10.4049/jimmunol.155.11.5268}, number={11}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Connor, A M and Fanning, L J and Celler, J W and Hicks, L K and Ramsden, D A and Wu, G E}, year={1995}, month=dec, pages={5268–5272} }

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