DOI: 10.4049/jimmunol.155.1.259. Genetic immunization against herpes simplex virus. Protection is mediated by CD4+ T lymphocytes

Genetic immunization against herpes simplex virus. Protection is mediated by CD4+ T lymphocytes

10.4049/jimmunol.155.1.259

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Plasmid DNA encoding proteins represent a convenient novel approach to vaccination. We have investigated this “genetic immunization” approach as a means to protect against herpes simplex virus (HSV) infection using a mouse zosteriform model that mimics several aspects of reactivated HSV infection of humans. After i.m. immunization with plasmid DNA-encoding glycoprotein B (gB), (pc-gB), 80% of BALB/c mice were completely protected and lesions were delayed in the remaining animals. Upon pc-gB vaccination, the animals developed both gB- and HSV-specific IgG Ab response and the isotype examination revealed a predominance of IgG2a. These mice also have low levels (1/16) of HSV-neutralizing Abs. Immune splenocytes obtained from pc-gB-immunized mice, when restimulated in vitro with HSV resulted in production of type 1 cytokines. Evidence for CD(8+)-mediated cytotoxic T lymphocyte response was equivocal. Protection could be adoptively transferred to nude mice recipients by CD4+ T cells from pc-gB-immunized mice but not by CD8+ T cells. Our results demonstrate that genetic immunization is a potent means of inducing protection against HSV and that the mechanism of immunity responsible for clearing virus from cutaneous sites is principally by CD4+ T cells. It is likely that these cells are Th1 cells because type 1 cytokines were the major cytokines detected upon in vitro Ag stimulation.

Bibliography

Manickan, E., Rouse, R. J., Yu, Z., Wire, W. S., & Rouse, B. T. (1995). Genetic immunization against herpes simplex virus. Protection is mediated by CD4+ T lymphocytes. The Journal of Immunology, 155(1), 259â265.

Authors 5

E Manickan (first)
R J Rouse (additional)
Z Yu (additional)
W S Wire (additional)
B T Rouse (additional)

References 0 Referenced 239

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 31, 2022, 6:53 a.m.)
Deposited	5 months ago (March 29, 2025, 11:33 p.m.)
Indexed	1 month ago (July 30, 2025, 11:35 a.m.)
Issued	30 years, 2 months ago (July 1, 1995)
Published	30 years, 2 months ago (July 1, 1995)
Published Online	30 years, 2 months ago (July 1, 1995)
Published Print	30 years, 2 months ago (July 1, 1995)

Funders 0

None

BibTeX

@article{Manickan_1995, title={Genetic immunization against herpes simplex virus. Protection is mediated by CD4+ T lymphocytes}, volume={155}, ISSN={0022-1767}, url={http://dx.doi.org/10.4049/jimmunol.155.1.259}, DOI={10.4049/jimmunol.155.1.259}, number={1}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Manickan, E and Rouse, R J and Yu, Z and Wire, W S and Rouse, B T}, year={1995}, month=jul, pages={259–265} }

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