DOI: 10.4049/jimmunol.153.12.5525. Novel allele-specific, post-translational reduction in HLA class I surface expression in a mutant human B cell line.

Novel allele-specific, post-translational reduction in HLA class I surface expression in a mutant human B cell line.

10.4049/jimmunol.153.12.5525

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Human B cell line .220 has a novel defect in HLA class I cell surface expression. Mutant .220 was derived from .184TGr, from which both copies of HLA-A and -B are deleted, and has less surface HLA-C than .184TGr. Transfer of class I genes into .220 revealed allele-specific reductions in surface expression: HLA-A1 and -B8 were 1 to 21% of normal; HLA-A11, -A24, and -B5 were moderately reduced; and HLA-A2, -A3, and -B7 were reduced little, if at all. Class I mRNA in .220(A1) and .220(B8) transferents is normal in size and at least normal in quantity. Surface expression of class I molecules was restored by fusing .220 transferents with mutant .174, which lacks the TAP-1 and -2 genes needed for transport of class I-binding peptides. Fusion of .220(A1) cells with beta 2-microglobulin-deficient Daudi cells also fully restored surface expression of class I molecules encoded by both parental cells, indicating beta 2-microglobulin is functional in .220. Pulse-chase experiments showed transgene-encoded HLA-A1 and -B8 alpha-chains are made in apparently normal amounts and associate with beta 2-microglobulin in .220. However, post-translational processing of the HLA-A1 and -B8 molecules is retarded in or before the Golgi apparatus, and immunoprecipitable HLA-A1 molecules disappear after their synthesis. The effects of these abnormalities on surface expression of class I molecules were reversed by incubating .220(A1) and .220(B8) cells at 21 degrees C, which greatly increased the amounts of cell surface HLA-A1 and -B8.

Bibliography

Greenwood, R., Shimizu, Y., Sekhon, G. S., & DeMars, R. (1994). Novel allele-specific, post-translational reduction in HLA class I surface expression in a mutant human B cell line. The Journal of Immunology, 153(12), 5525â5536.

Authors 4

R Greenwood (first)
Y Shimizu (additional)
G S Sekhon (additional)
R DeMars (additional)

References 0 Referenced 113

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 31, 2022, 6:02 a.m.)
Deposited	8 months ago (Jan. 2, 2025, 11:32 a.m.)
Indexed	2 months, 1 week ago (June 27, 2025, 4:04 p.m.)
Issued	30 years, 8 months ago (Dec. 15, 1994)
Published	30 years, 8 months ago (Dec. 15, 1994)
Published Print	30 years, 8 months ago (Dec. 15, 1994)

Funders 0

None

BibTeX

@article{Greenwood_1994, title={Novel allele-specific, post-translational reduction in HLA class I surface expression in a mutant human B cell line.}, volume={153}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.153.12.5525}, DOI={10.4049/jimmunol.153.12.5525}, number={12}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Greenwood, R and Shimizu, Y and Sekhon, G S and DeMars, R}, year={1994}, month=dec, pages={5525–5536} }

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