DOI: 10.4049/jimmunol.152.9.4712. Target organ-specific up-regulation of the MRC OX-40 marker and selective production of Th1 lymphokine mRNA by encephalitogenic T helper cells isolated from the spinal cord of rats with experimental autoimmune encephalomyelitis.

Target organ-specific up-regulation of the MRC OX-40 marker and selective production of Th1 lymphokine mRNA by encephalitogenic T helper cells isolated from the spinal cord of rats with experimental autoimmune encephalomyelitis.

10.4049/jimmunol.152.9.4712

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Lewis x Buffalo F1 rat lymphocytes express both forms of the allelic marker RT7.1 (Lewis) and RT7.2 (Buffalo). We generated myelin basic protein (MBP)-specific encephalitogenic F1 T helper cell lines and adoptively transferred them into naive irradiated Lewis recipients, which enabled us to detect and isolate donor T cells (with RT7.2) within the recipients. The spinal cord and cerebrospinal fluid (CSF) were highly enriched for the donor T cells compared with the blood and spleen. The donor cell number peaked on the first day of disease in the spinal cord and CSF and decreased as the disease progressed. A high percentage of the donor T cells isolated from the spinal cord were positive for the T helper cell activation marker OX-40, whereas a (lower) percentage of CSF donor cells expressed OX-40. Donor cells isolated from blood or spleen were negative for OX-40 expression. In contrast, the IL-2 receptor (CD25) was positive on all the transferred T cells in all tissue sites examined. Cell-sorting experiments showed that the MBP-specific donor cells were enriched for IFN-gamma, IL-2, TNF-alpha, and IL-3 mRNA when compared with the host-recruited spinal cord cells, whereas similar amounts of IL-10 mRNA were produced by both populations. Lymphokine mRNA production was also enriched in donor T cells isolated from the spinal cord compared with donor T cells isolated from the spleen. The spinal cord donor cells produced higher levels of IL-2, IFN-gamma, and IL-3 mRNA, whereas similar amounts of IL-10 and TNF-alpha mRNA were produced from donor cells isolated from the spleen and the spinal cord. Our data suggest that the amount/percentage, activation state, and enhanced lymphokine production at the site of inflammation are all important factors in determining the autoimmune potential of Ag-specific effector T helper cells.

Bibliography

Weinberg, A. D., Wallin, J. J., Jones, R. E., Sullivan, T. J., Bourdette, D. N., Vandenbark, A. A., & Offner, H. (1994). Target organ-specific up-regulation of the MRC OX-40 marker and selective production of Th1 lymphokine mRNA by encephalitogenic T helper cells isolated from the spinal cord of rats with experimental autoimmune encephalomyelitis. The Journal of Immunology, 152(9), 4712â4721.

Authors 7

A D Weinberg (first)
J J Wallin (additional)
R E Jones (additional)
T J Sullivan (additional)
D N Bourdette (additional)
A A Vandenbark (additional)
H Offner (additional)

References 0 Referenced 79

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 31, 2022, 5:59 a.m.)
Deposited	5 months ago (March 31, 2025, 12:43 p.m.)
Indexed	2 months, 2 weeks ago (June 21, 2025, 12:30 a.m.)
Issued	31 years, 4 months ago (May 1, 1994)
Published	31 years, 4 months ago (May 1, 1994)
Published Online	31 years, 4 months ago (May 1, 1994)
Published Print	31 years, 4 months ago (May 1, 1994)

Funders 0

None

BibTeX

@article{Weinberg_1994, title={Target organ-specific up-regulation of the MRC OX-40 marker and selective production of Th1 lymphokine mRNA by encephalitogenic T helper cells isolated from the spinal cord of rats with experimental autoimmune encephalomyelitis.}, volume={152}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.152.9.4712}, DOI={10.4049/jimmunol.152.9.4712}, number={9}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Weinberg, A D and Wallin, J J and Jones, R E and Sullivan, T J and Bourdette, D N and Vandenbark, A A and Offner, H}, year={1994}, month=may, pages={4712–4721} }

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