DOI: 10.4049/jimmunol.146.7.2088. Human CD8+ T lymphocytes can be divided into CD45RA+ and CD45RO+ cells with different requirements for activation and differentiation

Human CD8+ T lymphocytes can be divided into CD45RA+ and CD45RO+ cells with different requirements for activation and differentiation

10.4049/jimmunol.146.7.2088

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract The functional distinction between CD45RA+ and CD45RO+ cells within the human CD4+ T cell subset is well established. This study was undertaken to investigate whether a similar division can be made within the CD8+ T cell population. A quantitative comparison was made of the requirements for activation and differentiation of CD8+CD45RA+ and CD8+CD45RO+ cells. Stimulation of T lymphocytes with anti-CD3 mAb immobilized at high-density induced strong proliferation and CTL activity in both CD45RA+ and CD45RO+ cells. Suboptimal TCR/CD3 triggering, in contrast, induced substantially higher levels of proliferation and CTL activity in CD8+CD45RO+ cells compared with their CD45RA+ counterparts. Lymphokine secretion (i.e., Il-2 and TNF-alpha) was under any condition more readily induced in CD8+CD45RO+ cells. Markedly, proliferation of both CD8+CD45RA+ and CD8+CD45RO+ T cells initiated by anti-CD3 mAb immobilized at high densities was not inhibited by addition of anti-CD25 mAb, in contrast to proliferation induced by suboptimal anti-CD3 mAb concentrations. These findings show that a functional division between CD45RA+ and CD45RO+ T cells with distinct requirements for activation and differentiation may also be made in the CD8+ subset.

Bibliography

de Jong, R., Brouwer, M., Miedema, F., & van Lier, R. A. (1991). Human CD8+ T lymphocytes can be divided into CD45RA+ and CD45RO+ cells with different requirements for activation and differentiation. The Journal of Immunology, 146(7), 2088â2094.

Authors 4

R de Jong (first)
M Brouwer (additional)
F Miedema (additional)
R A van Lier (additional)

References 0 Referenced 72

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 31, 2022, 3:58 a.m.)
Deposited	5 months, 1 week ago (March 29, 2025, 5:58 p.m.)
Indexed	3 months ago (June 1, 2025, 8 a.m.)
Issued	34 years, 5 months ago (April 1, 1991)
Published	34 years, 5 months ago (April 1, 1991)
Published Online	34 years, 5 months ago (April 1, 1991)
Published Print	34 years, 5 months ago (April 1, 1991)

Funders 0

None

BibTeX

@article{de_Jong_1991, title={Human CD8+ T lymphocytes can be divided into CD45RA+ and CD45RO+ cells with different requirements for activation and differentiation}, volume={146}, ISSN={0022-1767}, url={http://dx.doi.org/10.4049/jimmunol.146.7.2088}, DOI={10.4049/jimmunol.146.7.2088}, number={7}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={de Jong, R and Brouwer, M and Miedema, F and van Lier, R A}, year={1991}, month=apr, pages={2088–2094} }

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