DOI: 10.4049/jimmunol.143.10.3258. Comparison of the immunosuppressive properties of milk growth factor and transforming growth factors beta 1 and beta 2.

Comparison of the immunosuppressive properties of milk growth factor and transforming growth factors beta 1 and beta 2.

10.4049/jimmunol.143.10.3258

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract The effects of the newly isolated bovine milk growth factor (MGF) which shows N-terminal homology to transforming growth factor beta 2 were compared with the effects of porcine transforming growth factor beta 1 and beta 2 (pTGF-beta 1 and -beta 2) on human T lymphocyte activation. Freshly isolated human PBMC were stimulated with either PHA, anti-CD3 + phorbol-12,13-dibutyrate (PDBu), or with a combination of ionomycin + PDBu. MGF, pTGF-beta 1, and pTGF-beta 2 decreased mitogen-induced [3H]thymidine incorporation by 30 to 75% in a dose-dependent manner. The maximum degree of inhibition was obtained at 1 ng/ml (40 pM) and could not be increased by increasing the concentration of teh transforming growth factor 10-fold. Stimulation of fresh T cells with the recall Ag tetanus toxoid was also inhibited (85%) by MGF at pM concentrations as was the proliferation of a human T cell clone specific for purified protein derivative. The effects of MGF and pTGF-beta 1 on anti-CD3-mediated increase of intracellular Ca2+ (Cai2+) was investigated by using the Fura-2 method. Neither MGF nor pTGF-beta 1 inhibited this increase in Cai2+ induced by a mitogenic concentration of anti-CD3 antibody. In order to determine whether TGF-beta preferentially inhibited the CD4+ or CD8+ subpopulation of human T cells, a limiting dilution analysis system, which allows every T cell to proliferate, was used. pTGF-beta 1 at a concentration of 5 ng/ml decreased the frequency of proliferating T cell precursors of both the CD4+ and CD8+ subsets to a similar extent. Furthermore, MGF, pTGF-beta 1, and pTGF-beta 2 also decreased IL-2 mediated [3H]thymidine incorporation into human PBL Con A blasts and the IL-4-mediated [3H]thymidine incorporation of purified T lymphocytes costimulated with PDBu by 70%. In conclusion, bovine MGF exerts suppressive effects on human T cells stimulated with Ag, mitogens, or interleukins, and the degree of T cell suppression is similar (or identical) to those of pTGF-beta 1 or -beta 2.

Bibliography

Stoeck, M., Ruegg, C., Miescher, S., Carrel, S., Cox, D., Von Fliedner, V., & Alkan, S. (1989). Comparison of the immunosuppressive properties of milk growth factor and transforming growth factors beta 1 and beta 2. The Journal of Immunology, 143(10), 3258â3265.

Authors 7

M Stoeck (first)
C Ruegg (additional)
S Miescher (additional)
S Carrel (additional)
D Cox (additional)
V Von Fliedner (additional)
S Alkan (additional)

References 0 Referenced 47

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 30, 2022, 11:21 p.m.)
Deposited	8 months ago (Jan. 2, 2025, 12:30 p.m.)
Indexed	1 month ago (July 30, 2025, 10:18 a.m.)
Issued	35 years, 9 months ago (Nov. 15, 1989)
Published	35 years, 9 months ago (Nov. 15, 1989)
Published Print	35 years, 9 months ago (Nov. 15, 1989)

Funders 0

None

BibTeX

@article{Stoeck_1989, title={Comparison of the immunosuppressive properties of milk growth factor and transforming growth factors beta 1 and beta 2.}, volume={143}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.143.10.3258}, DOI={10.4049/jimmunol.143.10.3258}, number={10}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Stoeck, M and Ruegg, C and Miescher, S and Carrel, S and Cox, D and Von Fliedner, V and Alkan, S}, year={1989}, month=nov, pages={3258–3265} }

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