DOI: 10.4049/jimmunol.140.5.1393. A monoclonal antibody (NKI-L16) directed against a unique epitope on the alpha-chain of human leukocyte function-associated antigen 1 induces homotypic cell-cell interactions.

A monoclonal antibody (NKI-L16) directed against a unique epitope on the alpha-chain of human leukocyte function-associated antigen 1 induces homotypic cell-cell interactions.

10.4049/jimmunol.140.5.1393

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract In the present study a unique antibody (NKI-L16) reacting with the alpha-chain of the human leukocyte function-associated Ag-1 (LFA-1) is described, which stimulates homotypic cell-cell interactions in a manner very similar to 12-O-tetradecanoyl-phorbol-13-acetate (TPA), in contrast to other anti-LFA-1 mAb which inhibit cell aggregation. The induction of aggregate formation of EBV-transformed B cells (JY) and CTL clones by TPA or NKI-L16 is not accompanied by an increase in the expression of LFA-1. Nevertheless, this cluster formation is LFA-1 dependent, inasmuch as anti-LFA-1 antibodies, other than NKI-L16, completely abrogate aggregation. Simultaneous addition of NKI-L16 and TPA did not result in a further increase of the speed of cluster formation, suggesting that a similar pathway is activated. Immunoprecipitation and enzyme digestion studies revealed that NKI-L16 recognizes a unique epitope on the alpha-chain of LFA-1, most likely situated close to the transmembrane segment of the molecule. It is hypothesized that NKI-L16 or TPA can cause the LFA-1 molecule to convert from an inactive to an active configuration, thereby permitting binding of LFA-1 to its natural ligand.

Bibliography

Keizer, G. D., Visser, W., Vliem, M., & Figdor, C. G. (1988). A monoclonal antibody (NKI-L16) directed against a unique epitope on the alpha-chain of human leukocyte function-associated antigen 1 induces homotypic cell-cell interactions. The Journal of Immunology, 140(5), 1393â1400.

Authors 4

G D Keizer (first)
W Visser (additional)
M Vliem (additional)
C G Figdor (additional)

References 0 Referenced 163

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 31, 2022, 1:35 a.m.)
Deposited	8 months ago (Jan. 2, 2025, 1:04 p.m.)
Indexed	2 days, 13 hours ago (Sept. 3, 2025, 5:59 a.m.)
Issued	37 years, 6 months ago (March 1, 1988)
Published	37 years, 6 months ago (March 1, 1988)
Published Print	37 years, 6 months ago (March 1, 1988)

Funders 0

None

BibTeX

@article{Keizer_1988, title={A monoclonal antibody (NKI-L16) directed against a unique epitope on the alpha-chain of human leukocyte function-associated antigen 1 induces homotypic cell-cell interactions.}, volume={140}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.140.5.1393}, DOI={10.4049/jimmunol.140.5.1393}, number={5}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Keizer, G D and Visser, W and Vliem, M and Figdor, C G}, year={1988}, month=mar, pages={1393–1400} }

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