DOI: 10.4049/jimmunol.140.12.4308. Phosphorylation of T cell membrane proteins by activators of protein kinase C.

Phosphorylation of T cell membrane proteins by activators of protein kinase C.

10.4049/jimmunol.140.12.4308

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Activation of the enzyme protein kinase C (PKC) plays an important role in T cell activation. We investigated the phosphorylation of CD2, CD3, CD4, CD5, CD7, CD8, CD28 (Tp44), CD43 (sialophorin, gp115), and LFA-1 after incubation of human PBMC with the (PKC) activator PMA. These proteins were chosen for their role in transmembrane signal transduction (CD2, CD3, CD5, CD28, CD43), cell-cell interaction and adhesion (CD2, CD4, CD8, and LFA-1), or involvement in immunodeficiency states (CD43, CD7). CD5, CD7, CD43, and the alpha-chain of LFA-1 were found to be constitutively phosphorylated. PMA induced rapid hyperphosphorylation of CD5, CD7, and CD43, but not of the LFA-1 alpha-chain, and induced the phosphorylation of CD3, CD4, CD8 and of the LFA-1 beta-chain. PMA did not cause the phosphorylation of CD2 and CD28. PMA-induced phosphorylation was partially inhibited by the PKC inhibitor 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine dihydrochloride. Finally, the T cell activator Con A, which binds to the CD3/TCR complex was shown to induce a profile of protein phosphorylation similar to that observed with PMA. We conclude that PKC-mediated phosphorylation of T cell Ag may represent an important regulatory mechanism that governs the process of T cell activation.

Bibliography

Chatila, T. A., & Geha, R. S. (1988). Phosphorylation of T cell membrane proteins by activators of protein kinase C. The Journal of Immunology, 140(12), 4308â4314.

Authors 2

T A Chatila (first)
R S Geha (additional)

References 0 Referenced 68

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 31, 2022, 12:50 a.m.)
Deposited	7 months, 4 weeks ago (Jan. 2, 2025, 1:31 p.m.)
Indexed	7 months, 4 weeks ago (Jan. 2, 2025, 2:10 p.m.)
Issued	37 years, 2 months ago (June 15, 1988)
Published	37 years, 2 months ago (June 15, 1988)
Published Print	37 years, 2 months ago (June 15, 1988)

Funders 0

None

BibTeX

@article{Chatila_1988, title={Phosphorylation of T cell membrane proteins by activators of protein kinase C.}, volume={140}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.140.12.4308}, DOI={10.4049/jimmunol.140.12.4308}, number={12}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Chatila, T A and Geha, R S}, year={1988}, month=jun, pages={4308–4314} }

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