DOI: 10.4049/jimmunol.139.7.2414. Biologic-response-modifier-induced indoleamine 2,3-dioxygenase activity in human peripheral blood mononuclear cell cultures.

Biologic-response-modifier-induced indoleamine 2,3-dioxygenase activity in human peripheral blood mononuclear cell cultures.

10.4049/jimmunol.139.7.2414

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Degradation of tryptophan to kynurenine, catalyzed by indoleamine 2,3-dioxygenase (IDO), has been augmented in human epithelial cell lines treated with human interferon-gamma (HuIFN-gamma). Several human biologic response modifiers, including HuIFN-gamma, HuIFN-beta, HuIFN-alpha, interleukin 2 (HuIL-2), and tumor necrosis factor alpha, have now been assessed for their ability to enhance tryptophan degradation in human peripheral blood mononuclear cell (PMC) cultures. PMC were isolated from normal donors, cultivated in RPMI 1640 medium containing [3H]tryptophan, and treated with individual biologic response modifiers. At various intervals, culture supernatants were removed, fractionated by reversed-phase high performance liquid chromatography, and radioactivity in resultant fractions was determined. Significantly increased amounts of tryptophan catabolites were observed after treatment with HuIFN-gamma, HuIFN-beta, HuIFN-alpha, and HuIL-2, but not human tumor necrosis factor alpha. Often, greater than 30% of available tryptophan was degraded by treated PMC cultures. Although antibodies to HuIFN-alpha, HuIFN-beta, and HuIFN-gamma specifically neutralized the induction of IDO activity in PMC by their respective HuIFN, only anti-HuIFN-gamma antibody also neutralized HuIL-2-induced IDO activity. Furthermore, T24 bladder carcinoma cells, in which IDO was induced by HuIFN-gamma but not by the other biologic response modifiers, were induced to degrade tryptophan by supernatants of HuIL-2-stimulated PMC cultures, but not by HuIFN-beta-stimulated PMC culture supernatants. Thus, whereas HuIL-2 indirectly induced IDO in PMC cultures by stimulating production of HuIFN-gamma, all cases of interferons appeared to induce IDO directly in PMC cultures.

Bibliography

Carlin, J. M., Borden, E. C., Sondel, P. M., & Byrne, G. I. (1987). Biologic-response-modifier-induced indoleamine 2,3-dioxygenase activity in human peripheral blood mononuclear cell cultures. The Journal of Immunology, 139(7), 2414â2418.

Authors 4

J M Carlin (first)
E C Borden (additional)
P M Sondel (additional)
G I Byrne (additional)

References 0 Referenced 105

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 31, 2022, 12:16 a.m.)
Deposited	5 months ago (March 31, 2025, 4:44 p.m.)
Indexed	1 week, 3 days ago (Aug. 23, 2025, 1:25 a.m.)
Issued	37 years, 11 months ago (Oct. 1, 1987)
Published	37 years, 11 months ago (Oct. 1, 1987)
Published Online	37 years, 11 months ago (Oct. 1, 1987)
Published Print	37 years, 11 months ago (Oct. 1, 1987)

Funders 0

None

BibTeX

@article{Carlin_1987, title={Biologic-response-modifier-induced indoleamine 2,3-dioxygenase activity in human peripheral blood mononuclear cell cultures.}, volume={139}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.139.7.2414}, DOI={10.4049/jimmunol.139.7.2414}, number={7}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Carlin, J M and Borden, E C and Sondel, P M and Byrne, G I}, year={1987}, month=oct, pages={2414–2418} }

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