DOI: 10.4049/jimmunol.139.7.2317. Membrane interleukin 1 induction on human endothelial cells and dermal fibroblasts.

Membrane interleukin 1 induction on human endothelial cells and dermal fibroblasts.

10.4049/jimmunol.139.7.2317

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Human endothelial cells and dermal fibroblasts both expressed a membrane-associated interleukin 1 (IL-1) activity when stimulated with either recombinant tumor necrosis factor (TNF) or recombinant lymphotoxin but stimulated endothelial cells expressed significantly more membrane IL-1 per cell than did fibroblasts. Lipopolysaccharide induced membrane IL-1 activity on endothelial cells but not fibroblasts. Interferon-gamma treatment of endothelial cells and fibroblasts had no direct effect on membrane IL-1 expression and little effect when used as a pretreatment for TNF or lipopolysaccharide stimulation. Endothelial cell membrane IL-1 activity was induced within 24 hr of culture with TNF or lipopolysaccharide, and increased up to 72 hr of incubation. Antibodies raised against human monocyte-derived IL-1 species neutralized the membrane IL-1 activity of TNF-stimulated endothelial cells. Both absorption studies and neutralization with specific sera indicated that endothelial cell membrane IL-1 is structurally related to IL-1 alpha. Endothelial cells expressed both IL-1 alpha and IL-1 beta mRNA in response to TNF, lymphotoxin, and recombinant IL-1 species, as detected by Northern blot analysis. These studies demonstrate that endothelial cells can be activated to express a cell-surface IL-1 activity which is structurally, as well as functionally, related to the secreted form of IL-1.

Bibliography

Kurt-Jones, E. A., Fiers, W., & Pober, J. S. (1987). Membrane interleukin 1 induction on human endothelial cells and dermal fibroblasts. The Journal of Immunology, 139(7), 2317â2324.

Authors 3

E A Kurt-Jones (first)
W Fiers (additional)
J S Pober (additional)

References 0 Referenced 125

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 31, 2022, 12:17 a.m.)
Deposited	5 months ago (March 31, 2025, 4:44 p.m.)
Indexed	3 months, 1 week ago (May 20, 2025, 9:21 a.m.)
Issued	37 years, 11 months ago (Oct. 1, 1987)
Published	37 years, 11 months ago (Oct. 1, 1987)
Published Online	37 years, 11 months ago (Oct. 1, 1987)
Published Print	37 years, 11 months ago (Oct. 1, 1987)

Funders 0

None

BibTeX

@article{Kurt_Jones_1987, title={Membrane interleukin 1 induction on human endothelial cells and dermal fibroblasts.}, volume={139}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.139.7.2317}, DOI={10.4049/jimmunol.139.7.2317}, number={7}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Kurt-Jones, E A and Fiers, W and Pober, J S}, year={1987}, month=oct, pages={2317–2324} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        5,
        20
      ]
    ],
    "date-time": "2025-05-20T13:21:06Z",
    "timestamp": 1747747266152,
    "version": "3.40.3"
  },
  "reference-count": 0,
  "publisher": "Oxford University Press (OUP)",
  "issue": "7",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1987,
            10,
            1
          ]
        ],
        "date-time": "1987-10-01T00:00:00Z",
        "timestamp": 560044800000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "https://academic.oup.com/pages/standard-publication-reuse-rights"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1987,
        10,
        1
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>Human endothelial cells and dermal fibroblasts both expressed a membrane-associated interleukin 1 (IL-1) activity when stimulated with either recombinant tumor necrosis factor (TNF) or recombinant lymphotoxin but stimulated endothelial cells expressed significantly more membrane IL-1 per cell than did fibroblasts. Lipopolysaccharide induced membrane IL-1 activity on endothelial cells but not fibroblasts. Interferon-gamma treatment of endothelial cells and fibroblasts had no direct effect on membrane IL-1 expression and little effect when used as a pretreatment for TNF or lipopolysaccharide stimulation. Endothelial cell membrane IL-1 activity was induced within 24 hr of culture with TNF or lipopolysaccharide, and increased up to 72 hr of incubation. Antibodies raised against human monocyte-derived IL-1 species neutralized the membrane IL-1 activity of TNF-stimulated endothelial cells. Both absorption studies and neutralization with specific sera indicated that endothelial cell membrane IL-1 is structurally related to IL-1 alpha. Endothelial cells expressed both IL-1 alpha and IL-1 beta mRNA in response to TNF, lymphotoxin, and recombinant IL-1 species, as detected by Northern blot analysis. These studies demonstrate that endothelial cells can be activated to express a cell-surface IL-1 activity which is structurally, as well as functionally, related to the secreted form of IL-1.</jats:p>",
  "DOI": "10.4049/jimmunol.139.7.2317",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2022,
        12,
        31
      ]
    ],
    "date-time": "2022-12-31T05:17:12Z",
    "timestamp": 1672463832000
  },
  "page": "2317-2324",
  "source": "Crossref",
  "is-referenced-by-count": 125,
  "title": "Membrane interleukin 1 induction on human endothelial cells and dermal fibroblasts.",
  "prefix": "10.1093",
  "volume": "139",
  "author": [
    {
      "given": "E A",
      "family": "Kurt-Jones",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Pathology, Brigham and Women\u2019s Hospital and Harvard Medical School , Boston, MA 02115"
        }
      ]
    },
    {
      "given": "W",
      "family": "Fiers",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "State University of Ghent , Ghent ,",
          "place": [
            "Belgium"
          ]
        }
      ]
    },
    {
      "given": "J S",
      "family": "Pober",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Pathology, Brigham and Women\u2019s Hospital and Harvard Medical School , Boston, MA 02115"
        }
      ]
    }
  ],
  "member": "286",
  "published-online": {
    "date-parts": [
      [
        1987,
        10,
        1
      ]
    ]
  },
  "container-title": "The Journal of Immunology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://academic.oup.com/jimmunol/article-pdf/139/7/2317/62405015/2317.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://academic.oup.com/jimmunol/article-pdf/139/7/2317/62405015/2317.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2025,
        3,
        31
      ]
    ],
    "date-time": "2025-03-31T20:44:28Z",
    "timestamp": 1743453868000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://academic.oup.com/jimmunol/article/139/7/2317/8075847"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1987,
        10,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "7",
    "published-print": {
      "date-parts": [
        [
          1987,
          10,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.4049/jimmunol.139.7.2317",
  "relation": {},
  "ISSN": [
    "0022-1767",
    "1550-6606"
  ],
  "subject": [],
  "published-other": {
    "date-parts": [
      [
        1987,
        10
      ]
    ]
  },
  "published": {
    "date-parts": [
      [
        1987,
        10,
        1
      ]
    ]
  }
}