DOI: 10.4049/jimmunol.139.1.239. Suppressed expression of surface Ia on macrophages by lipopolysaccharide: evidence for regulation at the level of accumulation of mRNA.

Suppressed expression of surface Ia on macrophages by lipopolysaccharide: evidence for regulation at the level of accumulation of mRNA.

10.4049/jimmunol.139.1.239

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract The surface expression of class II major histocompatibility molecules (immune associated or Ia antigens) is an acquired property of macrophages, essential to their ability to interact effectively with T lymphocytes. Surface expression of Ia is induced by stimulants such as interferon-gamma and is suppressed by agents such as lipopolysaccharide (LPS). Recent studies on several cultured cell lines indicate that interferon-gamma can heighten cellular levels of mRNA encoding Ia, and the level of such mRNA may represent an important regulatory focus for controlling expression of surface Ia. Murine peritoneal macrophages were treated with interferon-gamma and/or LPS and expression of Ia mRNA determined by Northern blot analysis with a probe specific for the murine beta-chain of I-A. mRNA specific for I-A beta was not detectable in explanted macrophages obtained from sites of sterile inflammation but was induced by treatment of purified recombinant interferon-gamma. This effect was dose dependent and was optimal by 24 hr after stimulation. Ia-specific mRNA preceded the surface expression of Ia as monitored by a radioimmunoassay using a monoclonal antibody specific for I-A beta. When a physiologic dose of LPS was added concomitantly with the interferon-gamma, the time course of induction if Ia-specific mRNA was not altered, but the amount of such mRNA detected was suppressed 40 to 80%. This effect was dependent on the dose of LPS, and the levels of mRNA correlated closely with subsequent surface expression of Ia. The ability of LPS to suppress both mRNA and cell surface Ia expression required that the suppressive agent be added within 12 hr of the inducing stimulus. This is the time frame during which accumulation of mRNA occurs. Thus the data demonstrates that accumulation of specific mRNA is a major regulatory focus governing expression of Ia both by interferon-gamma and LPS.

Bibliography

Koerner, T. J., Hamilton, T. A., & Adams, D. O. (1987). Suppressed expression of surface Ia on macrophages by lipopolysaccharide: evidence for regulation at the level of accumulation of mRNA. The Journal of Immunology, 139(1), 239â243.

Authors 3

T J Koerner (first)
T A Hamilton (additional)
D O Adams (additional)

References 0 Referenced 40

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 30, 2022, 11:10 p.m.)
Deposited	5 months ago (March 31, 2025, 4:42 p.m.)
Indexed	5 months ago (April 1, 2025, 12:13 a.m.)
Issued	38 years, 2 months ago (July 1, 1987)
Published	38 years, 2 months ago (July 1, 1987)
Published Online	38 years, 2 months ago (July 1, 1987)
Published Print	38 years, 2 months ago (July 1, 1987)

Funders 0

None

BibTeX

@article{Koerner_1987, title={Suppressed expression of surface Ia on macrophages by lipopolysaccharide: evidence for regulation at the level of accumulation of mRNA.}, volume={139}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.139.1.239}, DOI={10.4049/jimmunol.139.1.239}, number={1}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Koerner, T J and Hamilton, T A and Adams, D O}, year={1987}, month=jul, pages={239–243} }

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